Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,835,662	+T	noncoding (2/76 nt)	unknown ←	tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,835,662	1	.	T	100.0%	110.5 / NA	38	noncoding (2/76 nt)	unknown	tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/23);  total (15/23)

TGGATTAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACC‑CGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTGCGGTCAGTTTTTTTATCATTTTTCTACTAATTAGGCGAATTGATACCAACAAAAAACCGGGCTTATACCCGGT  >  1/1835418‑1835859                                                                                                                                                                                                                                                      |                                                                                                                                                                                                      tGGATTAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTa                                                                                                                                                                                                  >  1:113715/1‑251 (MQ=255) tGGATTAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTa                                                                                                                                                                                                  >  1:877597/1‑251 (MQ=255)    aTTAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACa                                                                                                                                                                                               <  1:339758/251‑1 (MQ=255)     ttAAGAGTCCACTGCTCTACCAGTCTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACAt                                                                                                                                                                                              <  1:596784/251‑1 (MQ=255)     ttAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACAt                                                                                                                                                                                              <  1:189520/251‑1 (MQ=255)      tAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATc                                                                                                                                                                                             <  1:140569/251‑1 (MQ=255)            tCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaacaa                                                                                                                                                                                       <  1:807452/251‑1 (MQ=255)            accgatgcgctACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTAGAACCTTCGACCAACGGATTGAAAGTCCGCTGCTCTAGCGAATGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaacaa                                                                                                                                                                                       <  1:394087/242‑1 (MQ=37)                  gCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaac                                                                                                                                                                                         <  1:826187/243‑1 (MQ=255)                     ctACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCtt                                                                                                                                                                              >  1:717230/1‑251 (MQ=255)                           gACTAGGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaac                                                                                                                                                                                         <  1:478279/234‑1 (MQ=255)                            tcTAAGCTAACGACCCATTATTCGGGTATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGAATGAGCTAACGGCCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATAc                                                                                                                                                                       <  1:765213/250‑1 (MQ=255)                              tAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTg                                                                                                                                                                     <  1:567791/251‑1 (MQ=255)                                               attCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATc                                                                                                                                                    <  1:352256/251‑1 (MQ=255)                                                            aTTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaac                                                                                                                                                                                         <  1:852394/201‑1 (MQ=255)                                                                    aTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaacaa                                                                                                                                                                                       <  1:670779/195‑1 (MQ=255)                                                                    aTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaacaa                                                                                                                                                                                       <  1:493235/195‑1 (MQ=255)                                                                    aTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTataat                                                                                                                                                                         >  1:178716/1‑209 (MQ=255)                                                                    aTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGttttttt                                                                                                                               >  1:156858/1‑251 (MQ=255)                                                                      ggtggGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTg                                                                                                                                                                     <  1:467516/211‑1 (MQ=255)                                                                                      ttCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATAc                                                                                                             <  1:192689/251‑1 (MQ=255)                                                                                            ccTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCaa                                                                                                       >  1:278899/1‑251 (MQ=255)                                                                                                   ccAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTTACGAGCCATTATTTGGAATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATg                                                                                                <  1:193131/251‑1 (MQ=255)                                                                                                                      ctgctCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGAtt                                                                                                                                                                  <  1:153270/166‑1 (MQ=255)                                                                                                                        gctCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGa                                                                                                                                                                    <  1:112347/162‑1 (MQ=255)                                                                                                                        gctCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTg                                                                           >  1:684230/1‑251 (MQ=255)                                                                                                                         ctctACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTg                                                                           <  1:572564/250‑1 (MQ=255)                                                                                                                                    gAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATAc                                                                                                                                                                       >  1:318152/1‑147 (MQ=255)                                                                                                                                             aCCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATc                                                                                                                                                                                             <  1:593966/116‑1 (MQ=255)                                                                                                                                             aCCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATAc                                                                                                                                                                       >  1:730661/1‑138 (MQ=255)                                                                                                                                             aCCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATAc                                                                                                                                                                       <  1:83085/138‑1 (MQ=255)                                                                                                                                                    attTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACAt                                                                                                                                                                                              >  1:204700/1‑108 (MQ=255)                                                                                                                                                        gggATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAg                                                                                                     >  1:287286/1‑193 (MQ=255)                                                                                                                                                                                 gTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTaac                                                                                                                                                                                         >  1:836756/1‑84 (MQ=255)                                                                                                                                                                                 gTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTcaa                                                                                        <  1:70970/181‑1 (MQ=255)                                                                                                                                                                                 gTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTGCGGTCAGTTTTTTTATCATTTTTCTACTAATTAGGCGAATTGATACCAACaaaaaa                   >  1:638625/1‑250 (MQ=255)                                                                                                                                                                                 gTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTGCGGTCAGTTTTTTTATCATTTTTCTACTAATTAGGCGAATTGATACCAACaaaaaa                   >  1:316571/1‑250 (MQ=255)                                                                                                                                                                                                                               ctctACCGGCTGAGCTAACGACCTCGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTGCGGTCAGTTTTTTTATCATTTTTCTACTAATTAGGCGAATTGATACCAACAAAAAACCGGGCTTATACCCGGt  >  1:782680/1‑221 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                      TGGATTAAGAGTCCACTGCTCTACCAGACTAAGCTAACGACCCATTATTCGGATATGTTATTTAAAAATGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGACTGAGCTAACGACCCATTATTTGGGATATATTGTTTAAAAGTGGTGGGTCGTGAAGGATTCGAACCTTCGACCAACGGATTAAAAGTCCGCTGCTCTACCGGCTGAGCTAACGACC‑CGTTAACATCTAACAACGAGGGCTTATAATACTGATTTCAGTTTAATAATCAATAAAAAATTTTGTTTTTTTATTGATTTGTTTGAATACCATCAAAGATATGTATTTCAAACAAAATAAAGTGCGGTCAGTTTTTTTATCATTTTTCTACTAATTAGGCGAATTGATACCAACAAAAAACCGGGCTTATACCCGGT  >  1/1835418‑1835859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑