Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	726,574	Δ1 bp	intergenic (‑3860/‑60617)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	726,573	0	T	.	100.0%	67.9 / NA	17	intergenic (‑3859/‑60618)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Leu‑GAG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/9);  total (8/9)

TTTAGCAGCTAAGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAA  >  1/726453‑726816                                                                                                                         |                                                                                                                                                                                                                                                    gtatttaacattgTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTc                                                                                                                                                                                                                                              <  1:757877/115‑1 (MQ=255) gtatttaacattgTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatgta                                                                                                                                                                                       <  1:382401/170‑1 (MQ=255)   atttaacattgTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGt                                                                                                                                                                                                                                               >  1:497720/11‑124 (MQ=255)           ttgTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGaat                                                                                                                                                                                                              >  1:568183/3‑149 (MQ=255)             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                                                         <  1:566836/168‑1 (MQ=255)             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGtt                                                                                                                                               <  1:199993/210‑1 (MQ=255)             gTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGa                                                                                                      >  1:225005/1‑251 (MQ=255)               cTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAggg                                                                                                                                          >  1:497732/1‑213 (MQ=255)                                                              gAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGt                                                                                                                                                                                                                                               <  1:408451/65‑1 (MQ=255)                                                                           gtgtCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                                                         >  1:693226/1‑106 (MQ=255)                                                                           gtgtCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtata                                                                                                                                                                                           <  1:666090/104‑1 (MQ=255)                                                                                     tttaATAAGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGaaa                                                                                                                                                                                                                                        <  1:498269/49‑1 (MQ=255)                                                                                       taataaGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGt                                                                                                                                                                                                                                               >  1:3041/1‑40 (MQ=255)                                                                                       taataaGCTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGATATcgcg                            >  1:354355/1‑251 (MQ=255)                                                                                              cTTGTGGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTg                                                                                                                                    >  1:151415/1‑140 (MQ=255)                                                                                                tgtgGAATTAAATAAACGACCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACtatgtatatg                                                                                                                                                                                         <  1:744965/85‑1 (MQ=255)                                                                                                                  aCCGGTA‑TATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGaa  <  1:232544/250‑1 (MQ=255)                                                                                                                         |                                                                                                                                                                                                                                                    TTTAGCAGCTAAGTCTTAATGCTCCTTTGTTGTTTATAGAAAATAAAATTCAAGAATCCTTGAGTTTGAACTTAGTGTCATTTATTTAATAAGCTTGTGGAATTAAATAAACGACCGGTATTATAGTCCTGAAAAATTAAAATGCAAGTTTTTTTAGAATAATAAATTCACTATGTATATGTACGTATTGTGATTGCGAAAACTTTTTAATTAACAGTATGTTAAGGGTTATTGTAAATGAGCTATTTCTACTTTCTCTATTGATTTTACAGTAAATTTACAACTTTTAACTTTAATTACAAAAACACTTAAATATGTGTGGATTTAGAGATATCGCGATTTATTTCTTTTTTCTTTGTAGGAA  >  1/726453‑726816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑