Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,318,504	Δ1 bp	intergenic (+179374/+74807)	unknown → / ← unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,318,504	0	C	.	100.0%	106.3 / NA	26	intergenic (+179374/+74807)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/14);  total (12/14)

AGGTGTAAAAGGGGTTAGTTATGTGTAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTTCTTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGCCATAGCGTTCGGAATTACGGGATTTTACCGGTTTG  >  1/1318408‑1318745                                                                                                 |                                                                                                                                                                                                                                                  cttttgttttggcaatgttgccgtatAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCAt                                                                                        <  1:202826/226‑1 (MQ=255)                     ccgtatAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGgtg                                                                    <  1:464790/246‑1 (MQ=255)                       gtatAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGgtgt                                                                   >  1:649545/4‑250 (MQ=255)                        tatAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTcg                                                                 >  1:318322/3‑251 (MQ=255)                         atAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAg                                                                                                                                                                                                                     >  1:230483/2‑102 (MQ=255)                                  aGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTcacc                                                                                                                                                                 <  1:661117/145‑1 (MQ=255)                                   gCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGt                                                                                                                                   <  1:759998/174‑1 (MQ=255)                                   gCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAAt                                                                                             <  1:205287/212‑1 (MQ=255)                                   gCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTAtt                                                      >  1:246097/1‑251 (MQ=255)                                   gCCTAAAACATCGTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTGAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATaa                                                                                                                                      <  1:555805/171‑1 (MQ=255)                                    ccTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTAtt                                                      >  1:69073/1‑250 (MQ=255)                                     cTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTcgccg                                                              >  1:462443/1‑241 (MQ=255)                                        aaaCATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCAt                                                                                        <  1:57366/212‑1 (MQ=255)                                             tCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGAc                                                                                                                      >  1:394454/1‑177 (MQ=255)                                             tCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCAccc                                                                            >  1:692574/1‑219 (MQ=255)                                                  aaTCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGttt                                         >  1:158956/1‑249 (MQ=255)                                                     cAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGcc                                    <  1:835405/251‑1 (MQ=255)                                                       aCGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCggtcgtgaagg                                  <  1:165430/251‑11 (MQ=255)                                                          gACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGCCATAg                                <  1:119734/250‑1 (MQ=255)                                                              gCTGAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAAc                                                                                                                                           <  1:704460/139‑1 (MQ=255)                                                                 gAAATTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTcgccg                                                              <  1:178366/213‑1 (MQ=255)                                                                   aaTTAAAACCCAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAAGAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGCCATAGCGTTCGGAAt                      <  1:226603/251‑1 (MQ=255)                                                                           cccAGACGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCgg                                                                                                                                                                                           >  1:18605/1‑78 (MQ=255)                                                                                 cGGTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGCCATAg                                >  1:750057/1‑227 (MQ=255)                                                                                   gTTGGTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGt                                                                                                                                   <  1:49615/126‑1 (MQ=255)                                                                                       gTATGGCCTT‑TTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCATGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGCCATAGCGTTCGGAATTACGGGATTTTACCGGCTTg  >  1:177251/1‑251 (MQ=255)                                                                                                 |                                                                                                                                                                                                                                                  AGGTGTAAAAGGGGTTAGTTATGTGTAAACGGAAGCCTAAAACATCTTTAATCAACGGACCGCTGAAATTAAAACCCAGACGGTTGGTATGGCCTTCTTTGCTGTCTTCCGGTTGCTCGGTAAATAGATTTAAACTGCCGTGATATTCTGCGGTTGGTGCTTTGGTTTTAATGTTCACCACCCCGCCCATGGCGCCTGAACCATAACGTGCTGCTGCCGGACCTCGCAAGACTTCAATGGACTCAATAGCATCTGCCGGCACCCAGTTGGTGTCGCCGCGGGTATTCCGCATGCCGTTTCTGCCATAGCGTTCGGAATTACGGGATTTTACCGGTTTG  >  1/1318408‑1318745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑