| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 593,429 | 3 bp→CGA | intergenic (+300/+115) | unknown → / ← unknown | hypothetical protein/UPF0758 protein YicR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 593,429 | 0 | T | C | 84.2% | 24.9 / ‑0.2 | 20 | intergenic (+300/+117) | unknown/unknown | hypothetical protein/UPF0758 protein YicR |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (5/11); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
| * | 1 | 593,430 | 0 | T | G | 85.0% | 34.0 / 0.1 | 20 | intergenic (+301/+116) | unknown/unknown | hypothetical protein/UPF0758 protein YicR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (6/11); minor base A (2/1); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01 | |||||||||||
| * | 1 | 593,431 | 0 | G | A | 95.0% | 44.5 / NA | 20 | intergenic (+302/+115) | unknown/unknown | hypothetical protein/UPF0758 protein YicR |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (7/12); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTT > 1/593213‑593563 ||| cTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTTTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAg > 1:793873/1‑251 (MQ=255) cTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt < 1:126024/251‑5 (MQ=255) cTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt < 1:622206/251‑5 (MQ=255) tgtTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta < 1:821071/251‑10 (MQ=255) tgtTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta < 1:783339/251‑10 (MQ=255) aGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTTTTTATGTTTATATGATGATTCTGCGTTAAATTTTAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAgtgt > 1:322279/1‑235 (MQ=255) caaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaattattc < 1:543917/251‑18 (MQ=255) aaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATAC‑CCATCGTTTTTGTTTATGTTTATATGATGATTTTGCGTTAAATTTTAGGGGGGTTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt > 1:158898/1‑233 (MQ=255) caaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaattattcatac < 1:859192/251‑22 (MQ=255) gctgAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaatta < 1:482745/232‑15 (MQ=255) gAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTTTGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt > 1:826309/1‑215 (MQ=255) tGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgtt > 1:775368/1‑196 (MQ=255) gCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta > 1:440256/1‑171 (MQ=255) aaaTAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt < 1:258670/156‑5 (MQ=255) aTAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaat < 1:284319/162‑13 (MQ=255) gctCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaat < 1:366774/149‑13 (MQ=255) aaaCGGCGGGTTAATACTCCGCCGTTTTTCTTTTCATGTACATGATGATTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaatgt < 1:82718/203‑12 (MQ=255) tcgtTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt < 1:72476/90‑5 (MQ=255) tgatgaTTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAacac > 1:770647/1‑149 (MQ=255) gctttAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaa > 1:450714/4‑140 (MQ=255) ||| CTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTT > 1/593213‑593563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |