Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 926,823 G→A S230S (TCG→TCA)  unknown → 7‑cyano‑7‑deazaguanine synthase (EC 6.3.4.20)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1926,8230GA100.0% 185.7 / NA 64S230S (TCG→TCA) unknown7‑cyano‑7‑deazaguanine synthase (EC 6.3.4.20)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/35);  total (29/35)

AACCTTGCCATGGCTTACCCTTTTGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCGAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATA  >  1/926578‑927068
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
aaCCTTGCCATGGCTTACCCTTTTGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGa                                                                                                                                                                                                                                                  >  1:677499/1‑251 (MQ=255)
       ccATGGCTTACCCTTTTGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACAt                                                                                                                                                                                                                                           <  1:88001/251‑1 (MQ=255)
           ggCTTACCCTTTTGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAggg                                                                                                                                                                                                                                                   <  1:208117/239‑1 (MQ=255)
           ggCTTACCCTTTTGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACa                                                                                                                                                                                                                                            >  1:293831/1‑246 (MQ=255)
                     tttGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGtt                                                                                                                                                                                                                              >  1:502004/1‑250 (MQ=255)
                                 actccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGt                                                                                                                                                                                                                 <  1:638129/248‑1 (MQ=255)
                                 acacCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGt                                                                                                                                                                                                                 <  1:880123/251‑1 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACAt                                                                                                                                                                                                                                           >  1:320178/1‑222 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACAt                                                                                                                                                                                                                                           <  1:295952/222‑1 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTaaaaa                                                                                                                                                                                                                                    <  1:145501/229‑1 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGttcaattc                                                                                                                                                                                                                        <  1:477633/241‑1 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTtcaattcaat                                                                                                                                                                                                                     >  1:771130/1‑244 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAatat                                                                                                                                                                                                                   >  1:93893/1‑246 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAatat                                                                                                                                                                                                                   >  1:38932/1‑246 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCAt                                                                                                                                                                                                              >  1:270884/1‑251 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCAt                                                                                                                                                                                                              >  1:760219/1‑251 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCAt                                                                                                                                                                                                              >  1:654745/1‑251 (MQ=255)
                                    ccGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCAt                                                                                                                                                                                                              >  1:16452/1‑251 (MQ=255)
                                       ttAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCAtgtg                                                                                                                                                                                                           <  1:173692/251‑1 (MQ=255)
                                         aaTGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGaa                                                                                                                                                                                                         >  1:259947/1‑251 (MQ=255)
                                                                       gcTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCAtgtg                                                                                                                                                                                                           >  1:802732/1‑219 (MQ=255)
                                                                        cTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTg                                                                                                                                                                           <  1:532460/250‑1 (MQ=255)
                                                                          ggCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGa                                                                                                                                                                                                          >  1:345701/1‑217 (MQ=255)
                                                                           gCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGttt                                                                                                                                                                        <  1:375132/250‑1 (MQ=255)
                                                                              gATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAAt                                                                                                                                                                    >  1:369427/1‑251 (MQ=255)
                                                                               aTCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAAtt                                                                                                                                                                   <  1:837003/251‑1 (MQ=255)
                                                                                  aaCTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGt                                                                                                                                                                >  1:352803/1‑251 (MQ=255)
                                                                                         cgcgTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGtttaattt                                                                                                                                                         <  1:100095/251‑1 (MQ=255)
                                                                                                gATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAggg                                                                                                                                                                                                                                                   <  1:243966/154‑1 (MQ=255)
                                                                                                gATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATg                                                                                                                                                   >  1:812988/1‑250 (MQ=255)
                                                                                                 aTTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGc                                                                                                                                                  <  1:88163/250‑1 (MQ=255)
                                                                                                    atatCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGt                                                                                                                                                                          <  1:446533/223‑1 (MQ=255)
                                                                                                       tCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTg                                                                                                                                           <  1:132182/251‑1 (MQ=255)
                                                                                                       tCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTg                                                                                                                                           <  1:108031/251‑1 (MQ=255)
                                                                                                           ccgGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGtt                                                                                                                                       <  1:299490/251‑1 (MQ=255)
                                                                                                           ccgGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGt                                                                                                                                        <  1:79510/250‑1 (MQ=255)
                                                                                                           ccgGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGt                                                                                                                                        <  1:545733/250‑1 (MQ=255)
                                                                                                                   cccACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTaaaa                                                                                                                                                                                                                                     <  1:239969/149‑1 (MQ=255)
                                                                                                                       cacTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTg                                                                                                                           >  1:378085/1‑251 (MQ=255)
                                                                                                                           tGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTaaaaa                                                                                                                                                                                                                                    >  1:204096/1‑142 (MQ=255)
                                                                                                                           tGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGttcaattc                                                                                                                                                                                                                        <  1:298014/154‑1 (MQ=255)
                                                                                                                           tGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAAt                                                                                                                                                                                                       >  1:714255/1‑171 (MQ=255)
                                                                                                                           tGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGc                                                                                                                                                  >  1:865188/1‑224 (MQ=255)
                                                                                                                           tGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTAt                                                                                                                                             <  1:877326/229‑1 (MQ=255)
                                                                                                                                     gtgtCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTg                                                                                                                                           >  1:106673/1‑221 (MQ=255)
                                                                                                                                     gtgtCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTa                                                                                                             >  1:896977/1‑251 (MQ=255)
                                                                                                                                      tgtCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTAc                                                                                                            <  1:326177/251‑1 (MQ=255)
                                                                                                                                        tCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTCTGAGAGTGAATATGTAcc                                                                                                           <  1:573585/250‑1 (MQ=255)
                                                                                                                                                 gTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAggg                                                                                                                                                                                                                                                   >  1:478468/1‑105 (MQ=255)
                                                                                                                                                 gTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTg                                                                                                                                           <  1:545243/209‑1 (MQ=255)
                                                                                                                                                       gcgAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATCGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGt                                                                                                                                        <  1:452028/206‑1 (MQ=255)
                                                                                                                                                                    ttGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAgt                                                                              <  1:304829/251‑1 (MQ=255)
                                                                                                                                                                    ttGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAgt                                                                              <  1:889504/251‑1 (MQ=255)
                                                                                                                                                                             gcgcGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACtt                                                                     >  1:201156/1‑251 (MQ=255)
                                                                                                                                                                                  aaCGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTAcc                                                                                                           <  1:526273/208‑1 (MQ=255)
                                                                                                                                                                                    cGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAatat                                                                                                                                                                                                                   >  1:872546/1‑102 (MQ=255)
                                                                                                                                                                                    cGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTAt                                                                                                                                             <  1:869290/172‑1 (MQ=255)
                                                                                                                                                                                               ggCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGtt                                                   >  1:701855/1‑251 (MQ=255)
                                                                                                                                                                                                       tGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGc                                                                 <  1:516676/229‑1 (MQ=255)
                                                                                                                                                                                                                       aaaaaTGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTc                           <  1:787711/251‑1 (MQ=255)
                                                                                                                                                                                                                            tGCCTGAGAAAACAACCGCACTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTaa                      <  1:502696/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aCTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCat   <  1:697341/251‑1 (MQ=255)
                                                                                                                                                                                                                                                cTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTc                                                  >  1:726485/1‑203 (MQ=255)
                                                                                                                                                                                                                                                cTTTCAAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCata  >  1:136827/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
AACCTTGCCATGGCTTACCCTTTTGAAATCCGCACACCGTTAATGTGGCTGGATAAAAAAGAAACCTGGGCGCTGGCGGATCAACTTGGCGCGTTTGATTATATCCGCCGGCACACCCACACTTGCTACAACGGTGTCGAAGGCGGTTGCGGCGAATGCCCGAGTTGCGTTTTGCGCGAACGTGGATTGAAGGCGTATTTGCCGGAACGTGAGGAAAAAATGCCTGAGAAAACAACCGCACTTTCGAGGGAGTAACATTTAAAAATTAGTTCAATTCAATATGTCATGTGAAATTAAACTTAAAAATTCACAGTTTCCCTTGTTTAAATTAGTTTAATTTAATATGCTGTATTGAGTTAAACTAATTTTGAGAGTGAATATGTACCTTTCCCGCCAAATCCAACCTTTAGTAAGTCGTCTACTTAAGCAATTTCCGGCAGTTCTTGTCACCGGTGCGAGACAGGTCGGTAAATCGACGTTGTTAAAGCATA  >  1/926578‑927068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑