Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	150,529	A→G	intergenic (+54/‑1)	unknown → / → unknown	Phage protein (ACLAME 1194)/Phage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	150,529	0	A	G	100.0%	58.3 / NA	24	intergenic (+54/‑1)	unknown/unknown	Phage protein (ACLAME 1194)/Phage protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/12);  total (12/12)

ATACCGGATGGTGCAGAATATTTGTACTATTTGGGGTCGGATCTAAACCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAAATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCTTGAGCTATGC  >  1/150293‑150601                                                                                                                                                                                                                                             |                                                                         aTACCGGATGGTGCAGAATATTTGTACTATTTGGGGTCGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATAttt                                                            >  1:755179/1‑251 (MQ=37)           gTGCAGAATATTTGTACTATTTGGGGTCGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTgg                                                  >  1:488604/1‑251 (MQ=37)                  atatTTGTACTATTTGGGGTCGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGtt                                           >  1:243246/1‑251 (MQ=37)                   tatTTGTACTATTTGGGGTCGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGtt                                           >  1:28989/1‑250 (MQ=37)                   tatTTGTACTATTTGGGGTCGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGtt                                           >  1:635794/1‑250 (MQ=37)                        gTACTATTTGGGGTCGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTtata                                                        >  1:385155/1‑232 (MQ=255)                                      cGGATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAAc                       <  1:267404/251‑1 (MQ=37)                                        gATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAat                                                                >  1:90260/1‑208 (MQ=255)                                        gATCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATc                                                      <  1:32459/218‑1 (MQ=255)                                         aTCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACgg                     <  1:154325/250‑1 (MQ=25)                                         aTCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACgg                     <  1:861604/250‑1 (MQ=25)                                         aTCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACgg                     <  1:604989/250‑1 (MQ=25)                                         aTCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACgg                     <  1:527903/250‑1 (MQ=25)                                         aTCTAAGCCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACgg                     <  1:239978/250‑1 (MQ=25)                                              agccAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGtata               >  1:642602/3‑251 (MQ=21)                                                ccAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATc             <  1:589677/251‑1 (MQ=21)                                                ccAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATc             <  1:425696/251‑1 (MQ=21)                                                ccAGAAGCTGCTGGTAAAAAGCGTAAGAGACAAAGAGCATTTCCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATc             <  1:16559/251‑1 (MQ=21)                                                                          gagCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCt            <  1:109119/226‑1 (MQ=14)                                                                            gCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCtt           >  1:802243/1‑225 (MQ=14)                                                                                  gagCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCTTGAGc       >  1:622021/1‑223 (MQ=14)                                                                                    gCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACgg                     >  1:847225/1‑207 (MQ=14)                                                                                    gCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCTTGAGCTATGc  >  1:634350/1‑226 (MQ=14)                                                                                       ttACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAGATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCTTGAGCTATGc  <  1:675397/223‑1 (MQ=14)                                                                                                                                                                                                                                             |                                                                         ATACCGGATGGTGCAGAATATTTGTACTATTTGGGGTCGGATCTAAACCAGAAGCTGCTGGTAAAAAGCGTAAGAGCCAAAGAGCATTACCACTCGGCACTAGAGCATGGCAGATATTACAAATCAAAAGACGACGTAATCCAAGTTATTGAGATGTTAACTGGTAAACGGTATCAAGAGTAACAACAGACCGCGAAAGTGCGGTTTTTTATTTTTATCAAAACAGGAGAAATTCAAATGTTAAAATATTTTATATCTTGGTTATGTTCTTTTATCTATCCACTTAACGGTGTATATCTTGAGCTATGC  >  1/150293‑150601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑