| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 903,940 | A→G | V115V (GTA→GTG) | unknown → | UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase (EC 6.3.2.10) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 903,940 | 0 | A | G | 100.0% | 213.6 / NA | 71 | V115V (GTA→GTG) | unknown | UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase (EC 6.3.2.10) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/43); total (28/43) | |||||||||||
GCGGTCAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTAAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGTCATGGCGGCGCATTTGGAAGGTTTT > 1/903698‑904186 | gCGGTCAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAagag < 1:444364/249‑1 (MQ=255) cAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTg > 1:95087/1‑251 (MQ=255) aTAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAACTGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGc > 1:429114/1‑250 (MQ=255) gCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTgg < 1:318670/249‑1 (MQ=255) cGAAAATTTTGATGCGCACCATTATGTTGCCATAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGAt < 1:551083/250‑1 (MQ=255) aTTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTgac < 1:568931/251‑1 (MQ=255) cACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAg < 1:17105/224‑1 (MQ=255) aCCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattt < 1:148430/251‑1 (MQ=255) aCCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattt < 1:78520/251‑1 (MQ=255) ccATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAagag > 1:831827/1‑196 (MQ=255) ccATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattta > 1:831701/1‑251 (MQ=255) ccATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattta > 1:826713/1‑251 (MQ=255) ccATTATGTTGCCAAAGCTGCCGAACAAGGTCGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGt < 1:447319/201‑1 (MQ=255) ttGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGc < 1:261257/251‑1 (MQ=255) cTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaac < 1:205261/251‑1 (MQ=255) tGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaaca < 1:471499/251‑1 (MQ=255) gAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCAttt < 1:785541/190‑1 (MQ=255) gAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGacgacg > 1:648492/1‑220 (MQ=255) ggTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCgg > 1:102436/1‑251 (MQ=255) gTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAag < 1:666962/165‑1 (MQ=255) gtgtgGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGCGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTg > 1:327753/1‑251 (MQ=255) ccGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAgaga < 1:149301/142‑1 (MQ=255) ccGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaac < 1:110248/251‑1 (MQ=255) ccGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATCTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaac < 1:3258/251‑1 (MQ=255) gAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaaca > 1:189855/1‑251 (MQ=255) aaGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac < 1:116805/249‑1 (MQ=255) aaGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac < 1:407789/249‑1 (MQ=255) aaGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac < 1:870923/249‑1 (MQ=255) aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTc > 1:522395/1‑191 (MQ=255) aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTc > 1:433026/1‑191 (MQ=255) aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCgg < 1:597986/216‑1 (MQ=255) aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGt < 1:185744/238‑1 (MQ=255) aGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaacag > 1:427394/1‑250 (MQ=255) atatCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATaaa < 1:670237/251‑1 (MQ=255) atatCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATaaa < 1:303221/251‑1 (MQ=255) atCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAAtt < 1:412146/251‑1 (MQ=255) gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGc < 1:166471/137‑1 (MQ=255) gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaac > 1:435014/1‑190 (MQ=255) gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGa > 1:687272/1‑251 (MQ=255) gCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGa > 1:425906/1‑251 (MQ=255) tCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGaa > 1:512237/1‑251 (MQ=255) aGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGgcg > 1:171950/1‑251 (MQ=255) cAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCa > 1:418116/1‑251 (MQ=255) tcgtcTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATc < 1:395591/251‑1 (MQ=255) cTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCatat < 1:873422/251‑1 (MQ=255) ttGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATc < 1:668770/251‑1 (MQ=255) gCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGAt < 1:598966/95‑1 (MQ=255) ttGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGa < 1:825058/251‑1 (MQ=255) ggcTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacagaac < 1:891557/198‑1 (MQ=255) gCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAagag < 1:158076/78‑1 (MQ=255) gCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTaac > 1:773570/1‑150 (MQ=255) tGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGgcg < 1:807636/250‑1 (MQ=255) gAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacag > 1:859302/1‑175 (MQ=255) aaaaaaTTAACCCGAAAGTGGTGGCTATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCaa > 1:875085/1‑251 (MQ=255) tAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtg < 1:768766/251‑1 (MQ=255) aaCCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtgt < 1:316121/251‑1 (MQ=255) aaCCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtgt < 1:500068/251‑1 (MQ=255) aaCCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGAtgt < 1:848530/251‑1 (MQ=255) gtggtgGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGt > 1:500630/1‑251 (MQ=255) gtggtgGCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGt > 1:873094/1‑251 (MQ=255) tggtggCGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTc < 1:82227/251‑1 (MQ=255) cGATGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCaataa < 1:150403/250‑1 (MQ=255) aTGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGt < 1:487068/251‑1 (MQ=255) aTGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGt < 1:474562/251‑1 (MQ=255) tGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGc > 1:686230/1‑64 (MQ=255) tGACGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTac < 1:579573/206‑1 (MQ=255) cGGGATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGTCATg > 1:302451/1‑251 (MQ=255) gggATCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTaacag < 1:437246/143‑1 (MQ=255) aTCTTCCGGTAAAACCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGa > 1:891681/1‑189 (MQ=255) aaaaCCACGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTtttattt > 1:708359/1‑72 (MQ=255) cGGTGAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTTGCGCTGGTCAATAATGTCATGGCGGCGCATTTGGAAGGtttt > 1:487136/1‑251 (MQ=255) | GCGGTCAATAAAGGGTTATTTTTTGCCCTTAAAGGCGAAAATTTTGATGCGCACCATTATGTTGCCAAAGCTGCCGAACAAGGTTGTGTGGCGGCGGTGGTTGAACATCCGACAGAAGGCAATATCGCCCGGCTTATCGTTAAAGACAGTCGTCTTGCTTTGGGGCGGCTTGCCAAATGGCTGCGTGAAAAAATTAACCCGAAAGTGGTGGCGATGACGGGATCTTCCGGTAAAACCACGGTAAAAGAGATGGTTGCCTCCATTTTGCAACAAAGTGCGGTGGATTCTGACGACGTTTTATTTACTCAAGGCAATTTTAACAACGACATCGGCGTGCCGCTCACTTTGTTACGTTTAACAGAACAGCATAAATTCGCGGTGATCGAACTCGGCGCCAATCATATCGGCGAAATCGCGTACACGACTTCCTTGGCGCAACCTGATGTGGCGCTGGTCAATAATGTCATGGCGGCGCATTTGGAAGGTTTT > 1/903698‑904186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |