Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	900,009	A→G	S21S (TCA→TCG)	unknown →	Cell division protein FtsL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	900,009	0	A	G	100.0%	173.3 / NA	61	S21S (TCA→TCG)	unknown	Cell division protein FtsL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/26);  total (35/26)

AAGCCAAGGCGAAGTCATTCCGAAAGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCAAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCAAC  >  1/899779‑900233                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  aaGCCAAGGCGAAGTCATTCCGAAAGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCtt                                                                                                                                                                                                               >  1:694355/1‑251 (MQ=255)               tCATTCCGAAAGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTg                                                                                                                                                                                                 <  1:353055/251‑1 (MQ=255)                  ttCCGAAAGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCa                                                                                                                                                                                              >  1:871713/1‑251 (MQ=255)                        aaGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTg                                                                                                                                                                                        <  1:6802/251‑1 (MQ=255)                        aaGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTg                                                                                                                                                                                        <  1:719264/251‑1 (MQ=255)                        aaGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTg                                                                                                                                                                                        <  1:246257/251‑1 (MQ=255)                                ccGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCgg                                                                                                                                                                                 >  1:239708/1‑250 (MQ=255)                                   ttACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGcac                                                                                                                                                                              <  1:495095/250‑1 (MQ=255)                                   ttACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGcac                                                                                                                                                                              <  1:758200/250‑1 (MQ=255)                                    tACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGGGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAg                                                                                                                                                                            <  1:431835/251‑1 (MQ=255)                                          aaGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGa                                                                                                                                                                      <  1:663241/251‑1 (MQ=255)                                          aaGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGa                                                                                                                                                                      <  1:337626/251‑1 (MQ=255)                                            gATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGa                                                                                                                                                                      >  1:98705/1‑249 (MQ=255)                                            gATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑tt                                                                                                                                                                   >  1:894048/1‑251 (MQ=255)                                            gATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑tt                                                                                                                                                                   >  1:657221/1‑251 (MQ=255)                                             aTCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑tt                                                                                                                                                                   <  1:267773/250‑1 (MQ=255)                                             aTCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑tt                                                                                                                                                                   <  1:652406/250‑1 (MQ=255)                                             aTCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑t                                                                                                                                                                    <  1:10811/249‑1 (MQ=255)                                                aaaTTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTAccc                                                                                                                                                               <  1:13674/251‑1 (MQ=255)                                                          cAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTAc                                                                                                                                                                                          >  1:517987/1‑215 (MQ=255)                                                                  ggTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGcac                                                                                                                                                                              <  1:707423/219‑1 (MQ=255)                                                                  ggTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGCGTCtgg                                                                                                                                             >  1:365641/1‑251 (MQ=255)                                                                   ctcggagatgtggtataagagacagaTGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCtggt                                                                                                                                            <  1:840417/226‑1 (MQ=255)                                                                                 gTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTAcc                                                                                                                                                                >  1:826981/1‑217 (MQ=255)                                                                                       cGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTgg                                                                                                                        >  1:320349/1‑251 (MQ=255)                                                                                                 gAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAgtgt                                                                                                                                                                         >  1:8125/1‑193 (MQ=255)                                                                                                    tGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCAc                                                                                                             <  1:350881/249‑1 (MQ=255)                                                                                                            agaTTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGcac                                                                                                                                                                              >  1:139755/1‑177 (MQ=255)                                                                                                              aTTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAg                                                                                                   <  1:384026/249‑1 (MQ=255)                                                                                                                      aaaCCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGaa                                                                                           <  1:846101/249‑1 (MQ=255)                                                                                                                              gagCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTtttaaatt                                                                                 >  1:368034/1‑251 (MQ=255)                                                                                                                               agCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTtttaaattt                                                                                >  1:432533/1‑251 (MQ=255)                                                                                                                               agCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTtttaaattt                                                                                >  1:230458/1‑251 (MQ=255)                                                                                                                                  cAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTtttaaatttaaa                                                                             >  1:668736/1‑251 (MQ=255)                                                                                                                                       gTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCtggtgg                                                                                                                                          >  1:321209/1‑185 (MQ=255)                                                                                                                                         gCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTg                                                                                                                                                                                        >  1:743354/1‑138 (MQ=255)                                                                                                                                            gtgtTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAagag                                                                   >  1:319019/1‑251 (MQ=255)                                                                                                                                            gtgtTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAagag                                                                   >  1:808309/1‑251 (MQ=255)                                                                                                                                                      ttGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGATTTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACaa                                                          >  1:807382/1‑251 (MQ=255)                                                                                                                                                          ggAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATc                                                                                                                                                                                                                 <  1:286481/96‑1 (MQ=255)                                                                                                                                                          ggAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGa                                                                                                                                                                      >  1:35427/1‑139 (MQ=255)                                                                                                                                                               gaATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTAtttt                                                                                                                                                                                                       <  1:245746/101‑1 (MQ=255)                                                                                                                                                                aaTAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTAccc                                                                                                                                                               >  1:814789/1‑139 (MQ=255)                                                                                                                                                                                              taCCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAgg                 <  1:702557/251‑1 (MQ=255)                                                                                                                                                                                               aCCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGc                <  1:209138/251‑1 (MQ=255)                                                                                                                                                                                                 ccGTTACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCAt              >  1:854363/1‑251 (MQ=255)                                                                                                                                                                                                    ttACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAg                                                                                                   <  1:180929/163‑1 (MQ=255)                                                                                                                                                                                                    ttACAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCa           >  1:401109/1‑251 (MQ=255)                                                                                                                                                                                                      aCAAGACATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATc                                                                                                                                                                                                                 <  1:472450/52‑1 (MQ=255)                                                                                                                                                                                                           aCATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGc                                                                                                                                                                                >  1:840820/1‑80 (MQ=255)                                                                                                                                                                                                           aCATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCa    >  1:299156/1‑251 (MQ=255)                                                                                                                                                                                                           aCATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCa    >  1:155294/1‑251 (MQ=255)                                                                                                                                                                                                            cATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCaa   >  1:817832/1‑251 (MQ=255)                                                                                                                                                                                                            cATTATTATGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCaa   >  1:643657/1‑251 (MQ=255)                                                                                                                                                                                                              ttattaTGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGAcaca                                                           >  1:388424/1‑193 (MQ=255)                                                                                                                                                                                                              ttattaTGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAg          <  1:776466/242‑1 (MQ=255)                                                                                                                                                                                                              ttattaTGGAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCAAc  >  1:798065/1‑250 (MQ=255)                                                                                                                                                                                                                attatGGAAGACTTATTTACGTCGAGTAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAgg                 >  1:113152/1‑233 (MQ=255)                                                                                                                                                                                                                      gAAGACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAg          <  1:109016/234‑1 (MQ=255)                                                                                                                                                                                                                         gACTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCAc                                                                                                             >  1:220383/1‑132 (MQ=255)                                                                                                                                                                                                                          aCTTATTTACGTCGAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGc                >  1:329988/1‑224 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                  AAGCCAAGGCGAAGTCATTCCGAAAGGGCTTCCGTTACGCGAAGATCAAATTCAGCGCAATCGGAGGTTAAAAGTGATCGGTAAAGCGATTATGCCGAGTGAAGCCGAGATTGTACAAAACCCAAGAGCCAGAAGTGCCGTGTTGCGCATTGCGGAGAGAATAAACTGATGTTGGAAAATAACGATAGATACCCGTTACAAGACATTATTATGGAAGACTTATTTACGTCAAATAAGTTTGTCTTTATCTTGTTATTTTTGATTGTCATTACTGCGCTCGGCACAGTGTGGA‑TTACCCATAAAACGCGGAGTCTGGTGGCGGAAAAAGGCGATTTGGTTCTTCAGCACCAAGCATTAGAAAACGAATTTTTAAATTTAAAATTGGAAGAGGCGACACAAAGCGATAACACTCGAATTGAAGCAATTGCCAAACAATTAGGCATGCAGCGTGCAAC  >  1/899779‑900233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑