Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	889,759	C→T	T288I (ACT→ATT)	unknown →	Transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	889,759	0	C	T	100.0%	205.9 / NA	69	T288I (ACT→ATT)	unknown	Transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/30);  total (39/30)

CGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTACTACCGCCGAGCAAAATCAAGACCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGTGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAT  >  1/889512‑890004                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtattac                                                                                                                                                                                                                                                    <  1:536819/251‑1 (MQ=255)         gCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAg                                                                                                                                                                                                                                             >  1:46354/1‑250 (MQ=255)                        aatcaattTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtattac                                                                                                                                                                                                                                                    >  1:489904/5‑228 (MQ=255)                        aatcaattTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtattac                                                                                                                                                                                                                                                    >  1:251601/5‑228 (MQ=255)                        aatcaattTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAg                                                                                                                                                                                                                             >  1:554248/5‑251 (MQ=255)                        aatcaattTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAg                                                                                                                                                                                                                             >  1:788486/5‑251 (MQ=255)                         atcaattTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAgg                                                                                                                                                                                                                            <  1:245689/248‑1 (MQ=255)                             attTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAg                                                                                                                                                                                                                        >  1:679804/1‑251 (MQ=255)                                         cacaTGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTacac                                                                                                                                                                                                             <  1:391504/250‑1 (MQ=255)                                         cacaTGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACAcc                                                                                                                                                                                                            <  1:566027/251‑1 (MQ=255)                                                   tCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCacaac                                                                                                                                                                                                  <  1:457468/251‑1 (MQ=255)                                                            tAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTacac                                                                                                                                                                                                             >  1:791186/1‑231 (MQ=255)                                                               cACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGaaa                                                                                                                                                                                      <  1:780242/251‑1 (MQ=255)                                                                   ggTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTacac                                                                                                                                                                                                             <  1:179449/224‑1 (MQ=255)                                                                         gAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAgccc                                                                                                                                                                                                                                <  1:496451/199‑4 (MQ=255)                                                                         gAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCacaac                                                                                                                                                                                                  <  1:880400/229‑1 (MQ=255)                                                                         gAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAg                                                                                                                                                                                                <  1:213304/231‑1 (MQ=255)                                                                         gAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAg                                                                                                                                                                                                >  1:691001/1‑231 (MQ=255)                                                                                     aGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATg                                                                                                                                                                  <  1:26194/249‑1 (MQ=255)                                                                                       ccAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGcc                                                                                                                                                              <  1:267099/251‑1 (MQ=255)                                                                                          aTTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCgt                                                                                                                                                           >  1:412985/1‑251 (MQ=255)                                                                                           tttCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCgtt                                                                                                                                                          >  1:232758/1‑251 (MQ=255)                                                                                                    gAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtattac                                                                                                                                                                                                                                                    <  1:320172/152‑1 (MQ=255)                                                                                                                        ggATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCa                                                                                                                             >  1:183042/1‑251 (MQ=255)                                                                                                                          aTCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCAtt                                                                                                                           >  1:370321/1‑251 (MQ=255)                                                                                                                                    caTTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGa                                                                                                                 >  1:706786/1‑251 (MQ=255)                                                                                                                                      tttATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAg                                                                                                                                                                                                <  1:240075/170‑1 (MQ=255)                                                                                                                                      tttATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCgttgtt                                                                                                                                                       >  1:622465/1‑211 (MQ=255)                                                                                                                                      tttATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAAt                                                                                                               >  1:317913/1‑251 (MQ=255)                                                                                                                                      tttATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAAt                                                                                                               >  1:546969/1‑251 (MQ=255)                                                                                                                                                ggTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACtt                                                                                                                                       <  1:894355/217‑1 (MQ=255)                                                                                                                                                   cAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtattac                                                                                                                                                                                                                                                    <  1:335968/105‑1 (MQ=255)                                                                                                                                                   cAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGaaa                                                                                                   >  1:714379/1‑250 (MQ=255)                                                                                                                                                   cAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGaaa                                                                                                   >  1:236026/1‑250 (MQ=255)                                                                                                                                                      cAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAAc                                                                                                  <  1:719508/248‑1 (MQ=255)                                                                                                                                                      cAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAg                                                                                                <  1:212377/250‑1 (MQ=255)                                                                                                                                                        aaGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTAt                                                                                             <  1:497313/251‑1 (MQ=255)                                                                                                                                                           gCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATcc                                                                                           <  1:37576/250‑1 (MQ=255)                                                                                                                                                                  ggctgCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATtg                                                                                   >  1:725690/1‑251 (MQ=255)                                                                                                                                                                   gctgCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATtgatga                                                                                                                      >  1:866171/1‑215 (MQ=255)                                                                                                                                                                   gctgCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATtg                                                                                   <  1:750674/250‑1 (MQ=255)                                                                                                                                                                         ggCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGccc                                                                                                                                                             >  1:459443/1‑170 (MQ=255)                                                                                                                                                                          gCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGt                                                                                               >  1:901495/1‑231 (MQ=255)                                                                                                                                                                          gCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTaaaa                                                                           >  1:594994/1‑251 (MQ=255)                                                                                                                                                                               gAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGa                                                                      >  1:632586/1‑251 (MQ=255)                                                                                                                                                                               gAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACC‑TGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTacac                                                                                                                                                                                                             <  1:459771/115‑1 (MQ=255)                                                                                                                                                                                 atatTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGaaa                                                                    >  1:234191/1‑251 (MQ=255)                                                                                                                                                                                      ggCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAg                                                                                                                    <  1:233758/198‑1 (MQ=255)                                                                                                                                                                                            cGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGgtcg                                                         <  1:522322/251‑1 (MQ=255)                                                                                                                                                                                                gTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCg                                                                                                                                                   >  1:684342/1‑157 (MQ=255)                                                                                                                                                                                                gTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGgtcgt                                                        >  1:505390/1‑248 (MQ=255)                                                                                                                                                                                                 ttttttGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTgc                                                    <  1:526903/251‑1 (MQ=255)                                                                                                                                                                                                 ttttttGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTgc                                                    <  1:61613/251‑1 (MQ=255)                                                                                                                                                                                                            aaTGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGc                                                                                        >  1:8395/1‑204 (MQ=255)                                                                                                                                                                                                              tGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAAt                                       <  1:649680/251‑1 (MQ=255)                                                                                                                                                                                                                             attattTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAAc                                                                                                  >  1:604405/1‑177 (MQ=255)                                                                                                                                                                                                                             attattTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATtg                                                                                   <  1:104828/192‑1 (MQ=255)                                                                                                                                                                                                                             attattTTCCGGTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCg                        >  1:183182/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAg                                                                                                                    >  1:146177/1‑148 (MQ=255)                                                                                                                                                                                                                                        gTAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGtt             >  1:153542/1‑251 (MQ=255)                                                                                                                                                                                                                                         tAAATACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGtt             <  1:436074/250‑1 (MQ=255)                                                                                                                                                                                                                                           aaTACGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGAc          <  1:198742/251‑1 (MQ=255)                                                                                                                                                                                                                                              aCGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAg                                                                          <  1:704890/184‑1 (MQ=255)                                                                                                                                                                                                                                              aCGCTGTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCAt       >  1:871373/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATtga                                                                                                                         >  1:185522/1‑132 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGgt                                                           >  1:285974/1‑194 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTc     >  1:272325/1‑248 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTc     <  1:358604/248‑1 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAt  >  1:8026/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAt  >  1:830780/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAt  >  1:586051/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAt  >  1:765487/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTTTATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAt  >  1:545798/1‑251 (MQ=255)                                                                                                                                                                                                                                                    tttATTACCGCCGAGCAAAATCAAGCCCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGCGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAt  <  1:137290/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTACTACCGCCGAGCAAAATCAAGACCAAGGCGAGCGTTTTTACACCGATGCACAACAGCGTTTTGAAAGTCGTCGCGTGGATCCTATGCGCCCGTTGTTACCGCCCGATAAACTTTGGTTGCGCATTGATGAAGTGAATCGTCACTTGAAACAGTATCCCGCTATTGTGCTAAAAGCGGAAAAAGTGCGGTCGTCTGCGCGGCAGAAAAATTTAGTGATTGCGTCGCTGCCACCGTTGACCATTCAAT  >  1/889512‑890004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑