Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 889,538 T→C I214I (ATT→ATC)  unknown → Transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1889,5380TC98.8% 250.0 / ‑4.7 84I214I (ATT→ATC) unknownTranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (48/35);  total (48/36)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTACTACCGCCGAG  >  1/889294‑889769
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       
tgttgATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTa                                                                                                                                                                                                                                   <  1:514247/251‑1 (MQ=255)
       taataaAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCgg                                                                                                                                                                                                                            >  1:782436/1‑251 (MQ=255)
                                gAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGAt                                                                                                                                                                                                        <  1:56571/246‑1 (MQ=255)
                                 aaTCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCAc                                                                                                                                                                                                   <  1:510705/250‑1 (MQ=255)
                                 aaTCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCAc                                                                                                                                                                                                   <  1:99935/250‑1 (MQ=255)
                                    ctctTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGt                                                                                                                                                                                               >  1:353081/1‑251 (MQ=255)
                                     tctTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTa                                                                                                                                                                                              <  1:199070/251‑1 (MQ=255)
                                           cctgcAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAt                                                                                                                                                                                        <  1:10788/247‑1 (MQ=255)
                                               cAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAttttt                                                                                                                                                                                    >  1:383390/1‑251 (MQ=255)
                                               cAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGCGCGCGGCGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAttttt                                                                                                                                                                                    >  1:635903/1‑251 (MQ=255)
                                                aaTTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTc                                                                                                                                                                                   <  1:621709/251‑1 (MQ=255)
                                                       gaaaGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGc                                                                                                                                                                             >  1:393552/2‑250 (MQ=255)
                                                            cGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAAttt                                                                                                                                                                       <  1:2754/251‑1 (MQ=255)
                                                             gggTTATCGTGCGGTGGAGCAGGCATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCAc                                                                                                                                                                                                   >  1:845840/1‑222 (MQ=255)
                                                                  aTCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGc                                                                                                                                                                                                     >  1:504158/1‑215 (MQ=255)
                                                                     gTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGaa                                                                                                                                                              >  1:130430/1‑251 (MQ=255)
                                                                          gTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCgg                                                                                                                                                         >  1:107371/1‑251 (MQ=255)
                                                                          gTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCgg                                                                                                                                                         >  1:219412/1‑251 (MQ=255)
                                                                            ggAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAACCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAt                                                                                                                                                                                        >  1:211541/1‑218 (MQ=255)
                                                                              aGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGa                                                                                                                                                      <  1:756290/250‑1 (MQ=255)
                                                                                   gTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAt                                                                                                                                                                                        >  1:790778/1‑211 (MQ=255)
                                                                                   gTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGa                                                                                                                                                      <  1:523510/245‑1 (MQ=255)
                                                                                   gTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCg                                                                                                                                                >  1:140463/1‑251 (MQ=255)
                                                                                   gTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCg                                                                                                                                                >  1:258389/1‑251 (MQ=255)
                                                                                    tATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGa                                                                                                                                               >  1:366442/1‑251 (MQ=255)
                                                                                      ttGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGAATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACg                                                                                                                                             >  1:726125/1‑251 (MQ=255)
                                                                                           aCATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATc                                                                                                                                        <  1:478510/251‑1 (MQ=255)
                                                                                                 cGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAac                                                                                                                                  <  1:203694/251‑1 (MQ=255)
                                                                                                   aaTATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAaca                                                                                                                                 >  1:637918/1‑250 (MQ=255)
                                                                                                   aaTATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAaca                                                                                                                                 >  1:81705/1‑250 (MQ=255)
                                                                                                   aaTATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAaca                                                                                                                                 >  1:784355/1‑250 (MQ=255)
                                                                                                   aaTATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAac                                                                                                                                  >  1:81991/1‑249 (MQ=255)
                                                                                                    atatGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAAcaca                                                                                                                               >  1:576603/1‑251 (MQ=255)
                                                                                                      atGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACAtt                                                                                                                             >  1:818647/1‑251 (MQ=255)
                                                                                                           gtgcgcggtgcgTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATc                                                                                                                         <  1:248233/250‑1 (MQ=255)
                                                                                                                  gtgcgTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGt                                                                                                                                                                                               <  1:559276/173‑1 (MQ=255)
                                                                                                                  gtgcgTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGt                                                                                                                  >  1:87728/1‑250 (MQ=255)
                                                                                                                       ttgttgGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAAc                                                                                                                      >  1:638059/1‑241 (MQ=255)
                                                                                                                       ttgttgGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTGTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCaa                                                                                                            <  1:438929/251‑1 (MQ=255)
                                                                                                                         gttgGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAAc                                                                                                                      <  1:686204/239‑1 (MQ=255)
                                                                                                                         gttgGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAg                                                                                                          >  1:318182/1‑251 (MQ=255)
                                                                                                                              aTTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTCGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAAttt                                                                                                                                                                                                                                    <  1:610668/124‑1 (MQ=255)
                                                                                                                                 tGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACgctg                                                                                                  <  1:2577/251‑1 (MQ=255)
                                                                                                                                  gTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAt                                                                                                                                                                                        >  1:314652/1‑164 (MQ=255)
                                                                                                                                        gATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCgg                                                                                           >  1:70923/1‑251 (MQ=255)
                                                                                                                                           gggAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAAtt                                                                                                                                                  >  1:375036/1‑193 (MQ=255)
                                                                                                                                           gggAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACgctggctg                                                                                              <  1:869633/245‑1 (MQ=255)
                                                                                                                                             gAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAt                                                                                                                                                                                        <  1:84768/153‑1 (MQ=255)
                                                                                                                                                 tGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAat                                                                                  <  1:135345/251‑1 (MQ=255)
                                                                                                                                                   cGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAata                                                                                 <  1:479393/250‑1 (MQ=255)
                                                                                                                                                     gTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAAc                                                                                                                      >  1:315710/1‑211 (MQ=255)
                                                                                                                                                     gTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCa                                                                                                       >  1:666784/1‑226 (MQ=255)
                                                                                                                                                     gTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATCTCCGACGGATAGCAc                                                                                                                                                                                                   <  1:680823/134‑1 (MQ=255)
                                                                                                                                                      tGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTgg                                                                             <  1:600370/251‑1 (MQ=255)
                                                                                                                                                      tGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTgg                                                                             <  1:138228/251‑1 (MQ=255)
                                                                                                                                                      tGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTgg                                                                             <  1:277956/251‑1 (MQ=255)
                                                                                                                                                       gCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGAc                                                                                                                                              <  1:57759/185‑1 (MQ=255)
                                                                                                                                                        ccTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGc                                                                            <  1:727816/250‑1 (MQ=255)
                                                                                                                                                        ccTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCa                                                                           <  1:766632/251‑1 (MQ=255)
                                                                                                                                                                   aGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGc                                                                                                              >  1:234803/1‑205 (MQ=255)
                                                                                                                                                                    gATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGaa                                                                                                                                                                                                                    <  1:709393/102‑1 (MQ=255)
                                                                                                                                                                                    aaaTTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAAc                                                <  1:743871/250‑1 (MQ=255)
                                                                                                                                                                                    aaaTTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAAc                                                <  1:369879/250‑1 (MQ=255)
                                                                                                                                                                                      aTTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCtt                                             >  1:589782/1‑251 (MQ=255)
                                                                                                                                                                                             ccATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCacc                                                                                                                      >  1:439194/1‑169 (MQ=255)
                                                                                                                                                                                             ccATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGc                                                                            >  1:509586/1‑213 (MQ=255)
                                                                                                                                                                                               aTTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAatta                                    <  1:702961/251‑1 (MQ=255)
                                                                                                                                                                                               aTTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAatta                                    >  1:492664/1‑251 (MQ=255)
                                                                                                                                                                                                      cTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTg                                                             >  1:232679/1‑219 (MQ=255)
                                                                                                                                                                                                      cTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGc                                                   >  1:550503/1‑229 (MQ=255)
                                                                                                                                                                                                      cTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCg                             >  1:856609/1‑251 (MQ=255)
                                                                                                                                                                                                              gtgGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATa                      >  1:133375/1‑250 (MQ=255)
                                                                                                                                                                                                               tgGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATAc                     <  1:113836/250‑1 (MQ=255)
                                                                                                                                                                                                                   cacCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAAc                                                                                                                      >  1:19815/1‑149 (MQ=255)
                                                                                                                                                                                                                     cccAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAata                                                                                 >  1:770496/1‑184 (MQ=255)
                                                                                                                                                                                                                     cccAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGttt              >  1:676177/1‑251 (MQ=255)
                                                                                                                                                                                                                       cAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCa                                                                                                                        >  1:822911/1‑143 (MQ=255)
                                                                                                                                                                                                                         gCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtatta          >  1:844545/1‑248 (MQ=255)
                                                                                                                                                                                                                         gCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtatta          >  1:505609/1‑248 (MQ=255)
                                                                                                                                                                                                                         gCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtatta          >  1:770730/1‑248 (MQ=255)
                                                                                                                                                                                                                          cGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtattac         <  1:536819/251‑1 (MQ=255)
                                                                                                                                                                                                                          cGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtatta          >  1:99145/1‑247 (MQ=255)
                                                                                                                                                                                                                          cGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTtatt           <  1:525320/249‑3 (MQ=255)
                                                                                                                                                                                                                                  gCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTATTACCGCCGAg  >  1:46354/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       
TGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTCCGTAGCCAATTTCGGGCGGAATTCGGGGAAATTCGACGGGATCCGGAACACATTTATCAACAGGTCAGCAAAGGCACGCTGGCTGCGGGCATTGAATATTGGCAGCCGCTGTTTTTTGAGCAAATGGCAACCTTGTTCAATTATTTTCCGGTAAATACGCTGTTTACTACCGCCGAG  >  1/889294‑889769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑