| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 889,349 | A→G | E151E (GAA→GAG) | unknown → | Transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 889,349 | 0 | A | G | 98.7% | 224.1 / ‑4.9 | 76 | E151E (GAA→GAG) | unknown | Transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (40/35); total (40/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACCTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTC > 1/889130‑889592 | ggAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTa < 1:665448/250‑1 (MQ=255) aCCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGa > 1:34864/1‑251 (MQ=255) aCCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGa < 1:234515/251‑1 (MQ=255) aCCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTG‑CCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGaa > 1:452732/1‑251 (MQ=255) cccTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTGTTGGaa < 1:424178/251‑1 (MQ=255) ccTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAAc < 1:871290/251‑1 (MQ=255) gCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATg > 1:532448/1‑251 (MQ=255) gTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcg > 1:44288/1‑251 (MQ=255) catcaAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcggtg < 1:827429/243‑1 (MQ=255) atatTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcggtgcgcggtg < 1:218237/243‑1 (MQ=255) tattatTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGc < 1:550759/186‑1 (MQ=255) tattatTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAAc > 1:680547/1‑218 (MQ=255) tattatTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACCTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTgg > 1:869456/1‑251 (MQ=255) ccGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGtt > 1:25659/1‑250 (MQ=255) cgcTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGAt > 1:865375/1‑251 (MQ=255) cgcTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGAt > 1:824628/1‑251 (MQ=255) cTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATgg > 1:732360/1‑251 (MQ=255) tGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTCCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGa < 1:217955/251‑1 (MQ=255) tttGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCgg < 1:577446/251‑1 (MQ=255) agttagtttaGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCa > 1:315661/10‑175 (MQ=255) ttGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCg > 1:285857/1‑251 (MQ=255) tGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGGAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGc < 1:204782/251‑1 (MQ=255) tGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGc < 1:765932/251‑1 (MQ=255) aGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTAt > 1:322180/1‑171 (MQ=255) aaCAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGAttttttt > 1:559771/1‑251 (MQ=255) cAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTtga < 1:125823/251‑1 (MQ=255) attttcattttACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTtgatgatga > 1:93556/1‑251 (MQ=255) ctttAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcgg > 1:589601/3‑156 (MQ=255) ctttAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACtttt > 1:144622/3‑251 (MQ=255) ttAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGcc > 1:215621/1‑200 (MQ=255) aaTGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGAtg < 1:743653/251‑1 (MQ=255) cAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGa > 1:299986/1‑251 (MQ=255) ggTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGAtgt > 1:155599/1‑243 (MQ=255) gTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACAccc < 1:876656/250‑1 (MQ=255) tGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCg < 1:780807/251‑1 (MQ=255) ccgTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGtt < 1:60036/251‑1 (MQ=255) ccgTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGt < 1:417880/250‑1 (MQ=255) gTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGc > 1:259301/1‑184 (MQ=255) gTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGAtg > 1:399941/1‑230 (MQ=255) gTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTg > 1:890931/1‑250 (MQ=255) ttATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCg > 1:198270/1‑240 (MQ=255) ttATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAgg < 1:544779/251‑1 (MQ=255) aTTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGaa < 1:485102/251‑1 (MQ=255) ttGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGAtg > 1:452504/1‑223 (MQ=255) ttGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAAt < 1:302861/251‑1 (MQ=255) cagcaTAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAAc < 1:865984/106‑1 (MQ=255) gcaTAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAAc > 1:856171/1‑104 (MQ=255) aTAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATgg > 1:897676/1‑106 (MQ=255) aaCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGTTGTGGACACCCAGCGTACGTTGCAGGAAATCGACCaaa > 1:520072/1‑251 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcggt < 1:149472/115‑1 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCAtt < 1:55231/200‑1 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGac > 1:502523/1‑218 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGtt < 1:430448/232‑1 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGtt > 1:627333/1‑232 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAaat > 1:690585/1‑250 (MQ=255) aCGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAaat > 1:223066/1‑250 (MQ=255) gTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAaatcaa > 1:775325/1‑248 (MQ=255) aTTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGaa > 1:360726/1‑94 (MQ=255) ttgttgATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCgttgt > 1:608749/1‑124 (MQ=255) tgttgATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTa < 1:514247/251‑1 (MQ=255) taataaAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTAc < 1:119162/216‑1 (MQ=255) taataaAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCgg > 1:782436/1‑251 (MQ=255) ataaAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTAt < 1:675272/59‑1 (MQ=255) aTCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGc < 1:592686/135‑1 (MQ=255) cGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCATTTCGCTTAGATTTTTTTGATGATGAAATTGATTc < 1:893172/170‑1 (MQ=255) gTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGa < 1:831016/70‑1 (MQ=255) gAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGAt < 1:56571/246‑1 (MQ=255) aaTCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCAc < 1:510705/250‑1 (MQ=255) aaTCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCAc < 1:99935/250‑1 (MQ=255) ctctTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGt > 1:353081/1‑251 (MQ=255) tctTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTa < 1:199070/251‑1 (MQ=255) cctgcAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAt < 1:10788/247‑1 (MQ=255) cAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCgttgt > 1:270148/1‑76 (MQ=255) cAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAttttt > 1:383390/1‑251 (MQ=255) cAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGCGCGCGGCGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAAttttt > 1:635903/1‑251 (MQ=255) aaTTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATCAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTc < 1:621709/251‑1 (MQ=255) | GGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACCTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTT‑CCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGAAATTGATTCCATTCGCACTTTTGATGTGGACACCCAGCGTACGTTGCAGGAAATCGACCAAATTAATTTATTGCCGGCACATGAATTTCCGACGGATAGCACGGGTATTGAATTTTTC > 1/889130‑889592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |