| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 889,247 | C→T | T117T (ACC→ACT) | unknown → | Transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 889,247 | 0 | C | T | 98.7% | 208.5 / ‑4.8 | 75 | T117T (ACC→ACT) | unknown | Transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (33/41); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
CGCGCAGTTTGACGGTCTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGCGAAAGAATTAACGCAACTGACGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACCTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGA > 1/889004‑889474 | cgcgCAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGc < 1:96925/251‑1 (MQ=255)cgcgCAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGc < 1:625003/251‑1 (MQ=255)cgcgCAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGc < 1:237532/251‑1 (MQ=255)cgcgCAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGc < 1:141273/251‑1 (MQ=255)cgcgCAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGc < 1:139884/251‑1 (MQ=255) cAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACg > 1:601373/1‑251 (MQ=255) cAGTTTGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACg > 1:254216/1‑251 (MQ=255) ttGATGGTTTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGtt < 1:164593/251‑1 (MQ=255) ggtttAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGcc > 1:701136/5‑250 (MQ=255) ggtttAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGcc > 1:42624/5‑250 (MQ=255) gtttAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGccc > 1:463466/4‑250 (MQ=255) gtttAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCccg > 1:661224/4‑251 (MQ=255) gtttAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCccg > 1:470996/4‑251 (MQ=255) gtttAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTAAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCccg > 1:450179/4‑251 (MQ=255) ccGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGt > 1:740596/1‑249 (MQ=255) gtggtgACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAATGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTAtt < 1:436793/250‑1 (MQ=255) gtgACCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTgc < 1:285476/250‑1 (MQ=255) aCCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTgcagca > 1:596105/1‑251 (MQ=255) aCCAATGACACCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTgcagc < 1:633776/250‑1 (MQ=255) acacCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGt < 1:581635/250‑1 (MQ=255) acacCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGt < 1:198065/250‑1 (MQ=255) acacCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGt < 1:151299/250‑1 (MQ=255) acacCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTa < 1:220330/251‑1 (MQ=255) cacCCGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAt > 1:355818/1‑251 (MQ=255) cccGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttg > 1:717776/1‑251 (MQ=255) ccGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:832728/251‑1 (MQ=255) ccGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:111340/251‑1 (MQ=255) ccGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:850166/251‑1 (MQ=255) ccGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:348723/251‑1 (MQ=255) ccGAAGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:17860/251‑1 (MQ=255) aaGTGCGGTGCGTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGGCTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgttg < 1:541702/250‑1 (MQ=255) gtgcggtgcgTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGAtt > 1:378112/1‑251 (MQ=255) gtgcggtgcgTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGAtt > 1:85694/1‑251 (MQ=255) cggtgcgTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATtaat < 1:473061/251‑1 (MQ=255) ggtgcgTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTgc < 1:282417/227‑1 (MQ=255) tgcgTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATtaataa < 1:650386/250‑1 (MQ=255) cgTTTGGAAAAAGAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAgg < 1:207399/251‑1 (MQ=255) gAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGt > 1:725181/1‑251 (MQ=255) gAATTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGt > 1:836198/1‑251 (MQ=255) aaTTAAAGCAACTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGt < 1:631229/250‑1 (MQ=255) aaCTGGCGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAAtct < 1:465051/251‑1 (MQ=255) ttCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:830520/206‑1 (MQ=255) ttattGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGa > 1:277842/1‑251 (MQ=255) gATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTgcag > 1:629631/1‑159 (MQ=255) gggAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCg > 1:18087/1‑187 (MQ=255) ggAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTa < 1:665448/250‑1 (MQ=255) aCCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGa < 1:234515/251‑1 (MQ=255) aCCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGa > 1:34864/1‑251 (MQ=255) aCCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTG‑CCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGaa > 1:452732/1‑251 (MQ=255) cccTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTGTTGGaa < 1:424178/251‑1 (MQ=255) ccTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAAc < 1:871290/251‑1 (MQ=255) gCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTAttgt < 1:496464/158‑1 (MQ=255) gCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATg > 1:532448/1‑251 (MQ=255) gTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGaa < 1:265387/179‑1 (MQ=255) gTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcg > 1:44288/1‑251 (MQ=255) catcaAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcggtg < 1:827429/243‑1 (MQ=255) atatTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATgcggtgcgcggtg < 1:218237/243‑1 (MQ=255) tattatTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGc < 1:550759/186‑1 (MQ=255) tattatTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAAc > 1:680547/1‑218 (MQ=255) tattatTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACCTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTgg > 1:869456/1‑251 (MQ=255) ccGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGtt > 1:25659/1‑250 (MQ=255) cgcTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGAt > 1:865375/1‑251 (MQ=255) cgcTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGAt > 1:824628/1‑251 (MQ=255) cTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATgg > 1:732360/1‑251 (MQ=255) tGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTCCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGa < 1:217955/251‑1 (MQ=255) tttGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCgg < 1:577446/251‑1 (MQ=255) gTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGcc < 1:307390/143‑1 (MQ=255) agttagtttaGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCa > 1:315661/10‑175 (MQ=255) ttGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCg > 1:285857/1‑251 (MQ=255) tGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGGAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGc < 1:204782/251‑1 (MQ=255) tGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGc < 1:765932/251‑1 (MQ=255) aGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTAt > 1:322180/1‑171 (MQ=255) aaCAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGAttttttt > 1:559771/1‑251 (MQ=255) cAAGGCATTTTCATTTTACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTtga < 1:125823/251‑1 (MQ=255) attttcattttACCTGTCGCCACTTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTGCAATTGGAGAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTtgatgatga > 1:93556/1‑251 (MQ=255) | CGCGCAGTTTGACGGTCTAACCGTGGTGGTGACCAATGACACCCGAAGTGCGGTGCGTTTGGCGAAAGAATTAACGCAACTGACGCCTTCCGAATTATTGCAAAACGTGACTTTTTTCCCCGATTGGGAAACCCTGCCTTATGATAGTTTTTCGCCTCATCAAGATATTATTTCCGCGCGCTTGAGCGCTTTGTTTCATTTGCAACAGCGCAAACAAGGCATTTTCATTTTACCTGTCGCCACCTTAATGCAACGGGTTTGCCCGCCGTCTTATTTGCAGCATAACGTATTGTTGATTAATAAAGGCGATCGTTTTGTCATCGAATCTCTTCGCCTACAATTGGAAAAAGCGGGTTATCGTGCGGTGGAGCAGGTATTGGAACATGGCGAATATGCGGTGCGCGGTGCGTTGTTGGATTTGTTCCCGATGGGAAGTGCGGTGCCTTTTCGCTTAGATTTTTTTGATGATGA > 1/889004‑889474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |