Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	150,070	C→G	L84L (CTC→CTG)	unknown →	Phage protein (ACLAME 1194)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	150,070	0	C	G	100.0%	66.6 / NA	29	L84L (CTC→CTG)	unknown	Phage protein (ACLAME 1194)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/15);  total (14/15)

AGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATA  >  1/149833‑150312                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   aGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACa                                                                                                                                                                                                                                       >  1:138690/1‑251 (MQ=255)                                                                                                         ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                                                <  1:45851/247‑1 (MQ=11)                                                                                                         ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                                                <  1:656074/247‑1 (MQ=11)                                                                                                           cttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGAt                                                                                                                                     >  1:65448/2‑243 (MQ=11)                                                                                                                                      ttGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGAttt                                                                                                  >  1:49810/1‑251 (MQ=255)                                                                                                                                        gCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTag                                                                                                        <  1:634013/243‑1 (MQ=255)                                                                                                                                                  gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTagag                                                                                                                                                       <  1:663032/186‑1 (MQ=11)                                                                                                                                                  gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                                                                      >  1:372580/1‑235 (MQ=11)                                                                                                                                                  gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCgg                                                                                      >  1:68208/1‑251 (MQ=255)                                                                                                                                                       ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAgaga                                                                                                                                                      >  1:23072/1‑182 (MQ=11)                                                                                                                                                       ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCAtgt                                                                                 >  1:617054/1‑251 (MQ=255)                                                                                                                                                             aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                                                                           >  1:813220/1‑251 (MQ=255)                                                                                                                                                             aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                                                                           >  1:862477/1‑251 (MQ=255)                                                                                                                                                                  gtgtTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAAcc                                                                       >  1:23451/1‑250 (MQ=255)                                                                                                                                                                      tgtCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                                                <  1:679958/189‑1 (MQ=11)                                                                                                                                                                        tctcGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGcc                                                                 <  1:473612/250‑1 (MQ=255)                                                                                                                                                                                   cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACgg                                                                                                                 <  1:565227/191‑1 (MQ=11)                                                                                                                                                                                   cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGcc                                                     <  1:104007/251‑1 (MQ=255)                                                                                                                                                                                     aGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                                                              >  1:373634/1‑208 (MQ=11)                                                                                                                                                                                          aGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGt                                              >  1:153904/1‑251 (MQ=255)                                                                                                                                                                                                   cgTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCt                                     <  1:853795/250‑1 (MQ=255)                                                                                                                                                                                                    gTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                                                                      <  1:232723/185‑1 (MQ=11)                                                                                                                                                                                                    gTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTa                                    >  1:604737/1‑251 (MQ=255)                                                                                                                                                                                                        gATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                                                              >  1:31273/1‑189 (MQ=11)                                                                                                                                                                                                         aTTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGcc                                <  1:765734/250‑1 (MQ=255)                                                                                                                                                                                                                            aGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGAt             <  1:598807/250‑1 (MQ=16)                                                                                                                                                                                                                            aGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGAt             <  1:755772/250‑1 (MQ=16)                                                                                                                                                                                                                                 aaGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGc        <  1:102854/250‑1 (MQ=16)                                                                                                                                                                                                                                      tttGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAata  <  1:372098/251‑1 (MQ=16)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   AGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCCAATCTACGAGATACCGGATGGTGCAGAATA  >  1/149833‑150312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑