Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	150,050	G→A	G78R (GGA→AGA)	unknown →	Phage protein (ACLAME 1194)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	150,050	0	G	A	100.0%	64.7 / NA	28	G78R (GGA→AGA)	unknown	Phage protein (ACLAME 1194)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/13);  total (15/13)

GGACAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCC  >  1/149809‑150282                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         ggaaaaTAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAg                                                                                                                                                                                                                                 >  1:155340/5‑251 (MQ=255)               tgaatgaaCAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTtgtactggaa                                                                                                                                                                                                                   <  1:83093/251‑8 (MQ=255)               tgaatgaaCAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTtgtactggaa                                                                                                                                                                                                                   <  1:401627/251‑8 (MQ=255)                         aGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACa                                                                                                                                                                                                         >  1:138690/1‑251 (MQ=255)                                                                                                                                 ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                  <  1:656074/247‑1 (MQ=11)                                                                                                                                 ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                  <  1:45851/247‑1 (MQ=11)                                                                                                                                   cttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGAt                                                                                                       >  1:65448/2‑243 (MQ=11)                                                                                                                                                              ttGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGAttt                                                                    >  1:49810/1‑251 (MQ=255)                                                                                                                                                                gCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTag                                                                          <  1:634013/243‑1 (MQ=255)                                                                                                                                                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTagag                                                                                                                         <  1:663032/186‑1 (MQ=11)                                                                                                                                                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                                        >  1:372580/1‑235 (MQ=11)                                                                                                                                                                          gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCgg                                                        >  1:68208/1‑251 (MQ=255)                                                                                                                                                                               ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAgaga                                                                                                                        >  1:23072/1‑182 (MQ=11)                                                                                                                                                                               ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCAtgt                                                   >  1:617054/1‑251 (MQ=255)                                                                                                                                                                                     aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                                             >  1:862477/1‑251 (MQ=255)                                                                                                                                                                                     aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                                             >  1:813220/1‑251 (MQ=255)                                                                                                                                                                                          gtgtTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAAcc                                         >  1:23451/1‑250 (MQ=255)                                                                                                                                                                                              tgtCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                                  <  1:679958/189‑1 (MQ=11)                                                                                                                                                                                                tctcGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGcc                                   <  1:473612/250‑1 (MQ=255)                                                                                                                                                                                                           cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACgg                                                                                   <  1:565227/191‑1 (MQ=11)                                                                                                                                                                                                           cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGcc                       <  1:104007/251‑1 (MQ=255)                                                                                                                                                                                                             aGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                                >  1:373634/1‑208 (MQ=11)                                                                                                                                                                                                                  aGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGt                >  1:153904/1‑251 (MQ=255)                                                                                                                                                                                                                           cgTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCt       <  1:853795/250‑1 (MQ=255)                                                                                                                                                                                                                            gTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                                        <  1:232723/185‑1 (MQ=11)                                                                                                                                                                                                                            gTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTa      >  1:604737/1‑251 (MQ=255)                                                                                                                                                                                                                                gATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                                >  1:31273/1‑189 (MQ=11)                                                                                                                                                                                                                                 aTTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGcc  <  1:765734/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         GGACAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGTGAATTGCCTAAGCC  >  1/149809‑150282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑