Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 150,027 A→C Q70P (CAG→CCG)  unknown → Phage protein (ACLAME 1194)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1150,0270AC100.0% 62.1 / NA 27Q70P (CAG→CCG) unknownPhage protein (ACLAME 1194)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/14);  total (13/14)

CTTTTTATTACTCAAAAAAGGACAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGT  >  1/149790‑150268
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 
cTTTTTATTTCCGAAAAAAGGAAAATAAAAAGTTGAATGAAAAAGAAAAAAACCCGTTTTTCAATGGCAATTACGGTATGAGGCAATCCGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCCCCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGAt                                                                                                                                                                                                                                      <  1:291177/251‑1 (MQ=14)
cTTTTTATTACTCAAAAAAGGAAAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGAt                                                                                                                                                                                                                                      <  1:75066/251‑1 (MQ=255)
                   ggaaaaTAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAg                                                                                                                                                                                                                   >  1:155340/5‑251 (MQ=255)
                                 tgaatgaaCAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTtgtactggaa                                                                                                                                                                                                     <  1:401627/251‑8 (MQ=255)
                                 tgaatgaaCAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTtgtactggaa                                                                                                                                                                                                     <  1:83093/251‑8 (MQ=255)
                                           aGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGAt                                                                                                                                                                                                                                            <  1:19921/202‑1 (MQ=255)
                                           aGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGAt                                                                                                                                                                                                                                      <  1:248483/208‑1 (MQ=255)
                                           aGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTCTTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACa                                                                                                                                                                                           >  1:138690/1‑251 (MQ=255)
                                                                                                                                                   ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                    <  1:45851/247‑1 (MQ=11)
                                                                                                                                                   ttcttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                    <  1:656074/247‑1 (MQ=11)
                                                                                                                                                     cttatCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGAt                                                                                         >  1:65448/2‑243 (MQ=11)
                                                                                                                                                                                ttGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGAttt                                                      >  1:49810/1‑251 (MQ=255)
                                                                                                                                                                                  gCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTag                                                            <  1:634013/243‑1 (MQ=255)
                                                                                                                                                                                            gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTagag                                                                                                           <  1:663032/186‑1 (MQ=11)
                                                                                                                                                                                            gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTagag                                                          >  1:372580/1‑235 (MQ=11)
                                                                                                                                                                                            gACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCgg                                          >  1:68208/1‑251 (MQ=255)
                                                                                                                                                                                                 ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAgaga                                                                                                          >  1:23072/1‑182 (MQ=11)
                                                                                                                                                                                                 ggACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCAtgt                                     >  1:617054/1‑251 (MQ=255)
                                                                                                                                                                                                       aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                               >  1:813220/1‑251 (MQ=255)
                                                                                                                                                                                                       aTCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCgag                               >  1:862477/1‑251 (MQ=255)
                                                                                                                                                                                                            gtgtTGTCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAAcc                           >  1:23451/1‑250 (MQ=255)
                                                                                                                                                                                                                tgtCTCGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATaa                                                                                    <  1:679958/189‑1 (MQ=11)
                                                                                                                                                                                                                  tctcGCTTTGGCAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGcc                     <  1:473612/250‑1 (MQ=255)
                                                                                                                                                                                                                             cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACgg                                                                     <  1:565227/191‑1 (MQ=11)
                                                                                                                                                                                                                             cAAGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGcc         <  1:104007/251‑1 (MQ=255)
                                                                                                                                                                                                                               aGACAAGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGAc                                                  >  1:373634/1‑208 (MQ=11)
                                                                                                                                                                                                                                    aGCCCGAACCGTTTGATTTAGATCGGGCGCTGAGAGGCGAAGCTTTTGTACTGGAAGGCGGAAACAAAGCTTATTTGCTTAAAAAATTAAAAACGTTTAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGt  >  1:153904/1‑251 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 
CTTTTTATTACTCAAAAAAGGACAATAAAATGTTGAATGAACAAGAAAAACACGCGTTTTTAAATGGCAATTACGGTATGACGCAATCGGGGGATAAGTGTAAACTGGTGTTAGTGGGCGAAACTCACCGAGATAATCCGTATTTGGTTATTATCACCAGAGGTGACGATTGGTCGTTGCAGTGGACTGACCAGGACCTATCATGTGTTGTCTCGCTTTGGCAAGACAAGCCCGAACAGTTTGATTTAGATCGGGCGCTGGGAGGCGAAGCTTTTGTGCTCGAAGGCGGAAACAAAGCTTTTTTGCTTAAAAAATTAAAAACGTTCAAATGTCATGATTTACTTGGTTATTATGAGTCCGAACCAGACTTAGAGATTGCCGCAGCATGGGATTATAAAGGTAATTTTGACGGTAGTCGTAGAGATTTAGACATTGTCGGCATGTGGCGAGAACCCAAGCCAATTAAGGCGCCTTTACGT  >  1/149790‑150268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑