Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	888,048	G→A	L581L (CTG→CTA)	unknown →	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	888,048	0	G	A	100.0%	190.5 / NA	67	L581L (CTG→CTA)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/40);  total (27/40)

TGCGGTTTTATTTTGGTGCATTTCAGCGAAGGCAAAGATGCAGGCAGCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGT  >  1/887803‑888292                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                                                                                                                                                    <  1:639961/249‑1 (MQ=255) tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                                                                                                                                                    <  1:672984/249‑1 (MQ=255) tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCCAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                                                                                                                                                    <  1:429372/249‑1 (MQ=255)     gTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCg                                                                                                                                                                                                                                              >  1:139986/1‑251 (MQ=255)     gTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCg                                                                                                                                                                                                                                              >  1:550499/1‑251 (MQ=255)                                       tgcgggcagCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaag                                                                                                                                                                                                            <  1:88031/247‑2 (MQ=255)                                         cgggcagCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaagta                                                                                                                                                                                                          <  1:458958/249‑4 (MQ=255)                                               gCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCggg                                                                                                                                                                                                    >  1:323976/1‑251 (MQ=255)                                                   cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaac                                                                                                                                                                                                <  1:205980/251‑3 (MQ=255)                                                   cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                                                                                                                 >  1:23021/1‑249 (MQ=255)                                                   cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                                                                                                                 >  1:858142/1‑249 (MQ=255)                                                   cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                                                                                                                 >  1:548670/1‑249 (MQ=255)                                                         ttCCGTGGTGGCGCTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGAtt                                                                                                                                                                                          >  1:728512/1‑251 (MQ=255)                                                             gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                                                                                                                                                       >  1:457608/4‑250 (MQ=255)                                                             gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                                                                                                                                                       >  1:214156/4‑250 (MQ=255)                                                              tggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCt                                                                                                                                                                                     <  1:616559/249‑1 (MQ=255)                                                                gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGc                                                                                                                                                                                                       <  1:752862/231‑1 (MQ=255)                                                                gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCttt                                                                                                                                                                                   >  1:332777/1‑251 (MQ=255)                                                                         tgtccGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGc                                                                                                                                                                          <  1:410682/248‑1 (MQ=255)                                                                                taaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGc                                                                                                                                                                   <  1:664681/249‑1 (MQ=255)                                                                                 aaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCa                                                                                                                                                                  <  1:551660/250‑1 (MQ=255)                                                                                  aCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaa                                                                                                                                                                 <  1:475604/251‑1 (MQ=255)                                                                                       gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                                                                                                             >  1:253026/8‑246 (MQ=255)                                                                                       gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                                                                                                             >  1:46631/8‑246 (MQ=255)                                                                                        cacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgtcc                                                                                                                                                           <  1:890464/245‑7 (MQ=255)                                                                                            ggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgat                                                                                                                                                       >  1:673041/3‑251 (MQ=255)                                                                                              cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCTGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                      <  1:441831/250‑1 (MQ=255)                                                                                              cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                      <  1:750548/250‑1 (MQ=255)                                                                                                 gCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACa                                                                                                                                                   <  1:806194/250‑1 (MQ=255)                                                                                                    ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatga                                                                                                                                                     <  1:537666/245‑1 (MQ=255)                                                                                                    ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCa                                                                                                                                               >  1:220851/1‑251 (MQ=255)                                                                                                      ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                             >  1:896113/1‑250 (MQ=255)                                                                                                      ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                                                                                                             >  1:216956/1‑250 (MQ=255)                                                                                                       gTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaagta                                                                                                                                                                                                          <  1:788098/189‑4 (MQ=255)                                                                                                        tGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAATGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaaat                                                                                                                                           <  1:442306/251‑4 (MQ=255)                                                                                                             cgggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                      <  1:298862/249‑1 (MQ=255)                                                                                                              gggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                                                                                                              >  1:463500/2‑223 (MQ=255)                                                                                                                            tttAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggttggcaaa                                                                                                                       >  1:739232/1‑243 (MQ=255)                                                                                                                                          gCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                         >  1:779751/1‑251 (MQ=255)                                                                                                                                           ccccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg                                                                                                         <  1:270092/250‑1 (MQ=255)                                                                                                                                             ccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGt                                                                                                        >  1:242533/1‑249 (MQ=255)                                                                                                                                               tttCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                    >  1:567562/1‑251 (MQ=255)                                                                                                                                                 tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaacaggat                                                                                                                                                                                           <  1:229641/159‑8 (MQ=255)                                                                                                                                                 tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                                                                                                                      <  1:593913/212‑1 (MQ=255)                                                                                                                                                  cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                                                                                                              <  1:745488/188‑4 (MQ=255)                                                                                                                                                  cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                 <  1:848411/249‑1 (MQ=255)                                                                                                                                                  cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGaaa                                                                                                 >  1:701517/3‑251 (MQ=255)                                                                                                                                                     tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                              <  1:785990/251‑1 (MQ=255)                                                                                                                                                     tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg                                                                                              <  1:679235/251‑1 (MQ=255)                                                                                                                                                      gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                  <  1:284703/214‑1 (MQ=255)                                                                                                                                                      gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag                                                                                                    <  1:770914/244‑1 (MQ=255)                                                                                                                                                         cccGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggt                                                                                                                              <  1:58294/215‑2 (MQ=255)                                                                                                                                                                      cAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                                                                                                                      >  1:268539/1‑178 (MQ=255)                                                                                                                                                                                              tGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAAt                                                     <  1:332150/251‑1 (MQ=255)                                                                                                                                                                                                    cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaag                                                                                                                                                                <  1:629123/138‑2 (MQ=255)                                                                                                                                                                                                    cTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAAc                                                <  1:152514/250‑1 (MQ=255)                                                                                                                                                                                                      cAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACgg                                              >  1:516656/1‑250 (MQ=255)                                                                                                                                                                                                                 accggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAagg                                  <  1:26268/240‑1 (MQ=255)                                                                                                                                                                                                                  ccggcaaggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                                                                                                                  <  1:515173/144‑1 (MQ=255)                                                                                                                                                                                                                         ggtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTAATGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                          >  1:122563/4‑249 (MQ=255)                                                                                                                                                                                                                          gtttGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaat                          <  1:261971/248‑3 (MQ=255)                                                                                                                                                                                                                               gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCaaatctgac                     <  1:82166/250‑8 (MQ=255)                                                                                                                                                                                                                               gCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATgac                     <  1:272769/250‑3 (MQ=255)                                                                                                                                                                                                                                             tttCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTaaaaa      >  1:599641/1‑251 (MQ=255)                                                                                                                                                                                                                                               tcttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTgaag                <  1:809826/239‑1 (MQ=255)                                                                                                                                                                                                                                                cttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAgg   >  1:164548/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ttcCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCTTTCTTATGATAACAACCCAAAATAAAACGGCATCGACTAAGGAGGCAAATATGACTGAAGAAGCTAAAAAAggt  <  1:50446/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      TGCGGTTTTATTTTGGTGCATTTCAGCGAAGGCAAAGATGCAGGCAGCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGGTTGCAAAAAAATTTCGCCTTCTTATGATAACAACCCAGAATAAAACGGCA‑CGACTAAGGAGGCAACTATGGCTGAAGAAGCCAAAAAAGGT  >  1/887803‑888292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑