Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	887,981	C→G	T559S (ACC→AGC)	unknown →	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	887,981	0	C	G	100.0%	196.4 / NA	68	T559S (ACC→AGC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (30/38);  total (30/38)

GATTTCCTTTCCGTCAGTTTCGGTTACACCCCCGAGCTGCTGGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAGGCAAAGATGCAGGCAGCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGG  >  1/887746‑888201                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             gATTTTCTTTCCGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGt                                                                                                                                                                                                                <  1:28006/250‑1 (MQ=255)          tccGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGa                                                                                                                                                                                                      <  1:25636/251‑1 (MQ=255)            cGTCAGTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGaag                                                                                                                                                                                                    <  1:8584/251‑2 (MQ=255)                 gTTTCGGTTACACCCCCGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGa                                                                                                                                                                                                >  1:622352/1‑250 (MQ=255)                             cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGaagc                                                                                                                                                                                                   <  1:571006/235‑3 (MQ=255)                             cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggccaggtttg                                                                                                                                                                                   >  1:855603/1‑238 (MQ=255)                             cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                                                                   >  1:38965/1‑238 (MQ=255)                             cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                                                                   >  1:497213/1‑238 (MQ=255)                             cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                                                                   >  1:766329/1‑238 (MQ=255)                             cccccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttg                                                                                                                                                                                   >  1:271732/1‑238 (MQ=255)                               cccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGaccggcaaggtttgg                                                                                                                                                                                  >  1:748746/1‑236 (MQ=255)                                ccGAACTGCTCGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCg                                                                                                                                                                                <  1:41675/251‑1 (MQ=255)                                                   gCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATttcttc                                                                                                                                                              <  1:383121/250‑1 (MQ=255)                                                      aaaGTGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCtac                                                                                                                                                          <  1:35151/251‑3 (MQ=255)                                                          tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                                                        <  1:639961/249‑1 (MQ=255)                                                          tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                                                        <  1:672984/249‑1 (MQ=255)                                                          tGCGGTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCCAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCg                                                                                                                                                        <  1:429372/249‑1 (MQ=255)                                                              gTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCg                                                                                                                                                  >  1:550499/1‑251 (MQ=255)                                                              gTTTTATTTTGGTGCATTTCAGCGAAAGCAAAGATGCGGGCAGCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCg                                                                                                                                                  >  1:139986/1‑251 (MQ=255)                                                                                                tgcgggcagCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaag                                                                                                                <  1:88031/247‑2 (MQ=255)                                                                                                  cgggcagCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaagta                                                                                                              <  1:458958/249‑4 (MQ=255)                                                                                                        gCGGCTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCggg                                                                                                        >  1:323976/1‑251 (MQ=255)                                                                                                            cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaac                                                                                                    <  1:205980/251‑3 (MQ=255)                                                                                                            cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                     >  1:23021/1‑249 (MQ=255)                                                                                                            cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                     >  1:548670/1‑249 (MQ=255)                                                                                                            cTGTTATTCCGTGGTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaa                                                                                                     >  1:858142/1‑249 (MQ=255)                                                                                                                  ttCCGTGGTGGCGCTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGAtt                                                                                              >  1:728512/1‑251 (MQ=255)                                                                                                                      gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                                                           >  1:457608/4‑250 (MQ=255)                                                                                                                      gtggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTc                                                                                           >  1:214156/4‑250 (MQ=255)                                                                                                                       tggTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCt                                                                                         <  1:616559/249‑1 (MQ=255)                                                                                                                         gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGc                                                                                                           <  1:752862/231‑1 (MQ=255)                                                                                                                         gTGGCGTTGTGTCCGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCttt                                                                                       >  1:332777/1‑251 (MQ=255)                                                                                                                                  tgtccGCTAACGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGc                                                                              <  1:410682/248‑1 (MQ=255)                                                                                                                                         taaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGc                                                                       <  1:664681/249‑1 (MQ=255)                                                                                                                                          aaCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCa                                                                      <  1:551660/250‑1 (MQ=255)                                                                                                                                           aCGGAGCACGGGCGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaa                                                                     <  1:475604/251‑1 (MQ=255)                                                                                                                                                gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                 >  1:46631/8‑246 (MQ=255)                                                                                                                                                gcacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgt                                                                 >  1:253026/8‑246 (MQ=255)                                                                                                                                                 cacgggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcgtcc                                                               <  1:890464/245‑7 (MQ=255)                                                                                                                                                     ggcGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgat                                                           >  1:673041/3‑251 (MQ=255)                                                                                                                                                       cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCTGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                          <  1:441831/250‑1 (MQ=255)                                                                                                                                                       cGGGCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                          <  1:750548/250‑1 (MQ=255)                                                                                                                                                          gCGTTGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACa                                                       <  1:806194/250‑1 (MQ=255)                                                                                                                                                             ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatga                                                         <  1:537666/245‑1 (MQ=255)                                                                                                                                                             ttGGTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCa                                                   >  1:220851/1‑251 (MQ=255)                                                                                                                                                               ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                 >  1:896113/1‑250 (MQ=255)                                                                                                                                                               ggTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaa                                                 >  1:216956/1‑250 (MQ=255)                                                                                                                                                                gTGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGaaagta                                                                                                              <  1:788098/189‑4 (MQ=255)                                                                                                                                                                 tGCAACGGGCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAATGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCaaaat                                               <  1:442306/251‑4 (MQ=255)                                                                                                                                                                      cgggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                          <  1:298862/249‑1 (MQ=255)                                                                                                                                                                       gggCGGAACAACAATTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                  >  1:463500/2‑223 (MQ=255)                                                                                                                                                                            gaacaacaaTTTAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCtacc                                                                                                                                                         <  1:740332/131‑4 (MQ=255)                                                                                                                                                                                     tttAAACGCAATTTGCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggttggcaaa                           >  1:739232/1‑243 (MQ=255)                                                                                                                                                                                                   gCCCCTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg             >  1:779751/1‑251 (MQ=255)                                                                                                                                                                                                    ccccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAg             <  1:270092/250‑1 (MQ=255)                                                                                                                                                                                                      ccTTTCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGt            >  1:242533/1‑249 (MQ=255)                                                                                                                                                                                                        tttCATTGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag        >  1:567562/1‑251 (MQ=255)                                                                                                                                                                                                          tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGaacaggat                                                                                               <  1:229641/159‑8 (MQ=255)                                                                                                                                                                                                          tcattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATaaaaa                                          <  1:593913/212‑1 (MQ=255)                                                                                                                                                                                                           cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCaagcg                                                                  <  1:745488/188‑4 (MQ=255)                                                                                                                                                                                                           cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGaaa     <  1:848411/249‑1 (MQ=255)                                                                                                                                                                                                           cattGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGCTGGCAAATTGTCGGTTGGAAGTGAAGaaa     >  1:701517/3‑251 (MQ=255)                                                                                                                                                                                                              tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg  <  1:785990/251‑1 (MQ=255)                                                                                                                                                                                                              tGCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTGAAGAAAggg  <  1:679235/251‑1 (MQ=255)                                                                                                                                                                                                               gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACg                                      <  1:284703/214‑1 (MQ=255)                                                                                                                                                                                                               gCACCCGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTGGTTGGCAAATTGTCGGTTGGAAGTgaag        <  1:770914/244‑1 (MQ=255)                                                                                                                                                                                                                  cccGCTGGCGACACAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCTGATGACATTCAAAATAAAAAAACGTggt                                  <  1:58294/215‑2 (MQ=255)                                                                                                                                                                                                                               cAATTTGCCGGAAGCGAAACGGATTGGTCGCTCAATGAAGCCGACCGGCAAGGTTTGGCGGATTTTGCCGATTTCTTCCTACCGTTGGCGTTTGTCTTGCCCGCTATTCGGCGCTTGATGAAAGTAAGCGGGGAACAGGATTTTCCTTTATTGGCAGCCTATTGCAAGCGTCCtgatg                                                          >  1:268539/1‑178 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             GATTTCCTTTCCGTCAGTTTCGGTTACACCCCCGAGCTGCTGGCGTTTTGGCAAAAGTGCGGTTTTATTTTGGTGCATTTCAGCGAAGGCAAAGATGCAGGCAGCGGCTGTTATTCCGTAGTGGCATTGTGCCCGCTGACGGAGCGCGGACGGGCGTTGGTGCAACAGGCGGAGCAACAATTTAAACGTAATCTGCCCCTTTCGTTGCACCCGCTGGCGACGCAATTTGTCGGAACCGAAACGGATTGGTCGCTCAATGAAACCGATTGGCAATGCTTGGCGGATTTTGCCGATTTCTTCCTGCCGTTGGCGTTTGCCTTGCCCGCTATTCGGCGCTTGATGAAATTAAGCGGGGAGCGGGATTTTCCTTTATTGACAGCCTATTGCAAACGCCCTGATGACATTCAACATAAAAAAACGTGGCTGAAAAATTGTCGGTTGGAAGTGAAGAAAGGG  >  1/887746‑888201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑