Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	887,319	C→T	Y338Y (TAC→TAT)	unknown →	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	887,319	0	C	T	100.0%	206.4 / NA	69	Y338Y (TAC→TAT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/37);  total (32/37)

ATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTCAGCCCGTTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGATGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGACGCAACCGCGTTACGATGCCAACCTTGCCAAACAGATTCAGATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGAATCGGTTTATGGCTTGCTGACCTTGGCGCATTACCGAACCTCGCCGTTGGATTTACGTCGATTATTGGATGCGCCGCGACAACAATTTTATCTTGCGCAGACGCAGGAGGCCTTGTTGGGTTGTGTGTGGGCTGTGCCTGAGGGCGAATTTGGTGACGACGCATTAATT  >  1/887084‑887553                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           aTACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGc                                                                                                                                                                                                                              >  1:462804/1‑250 (MQ=255)    cGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGccgaa                                                                                                                                                                                                                          >  1:595812/1‑248 (MQ=255)    cGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGccgaa                                                                                                                                                                                                                          >  1:279059/1‑248 (MQ=255)                          cTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACaaag                                                                                                                                                                                                    >  1:801365/1‑251 (MQ=255)                                  gcaTTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGAtt                                                                                                                                                                                            >  1:429962/3‑251 (MQ=255)                                     ttGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGTACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                                                                                                                                                                                           <  1:447481/249‑1 (MQ=255)                                     ttGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                                                                                                                                                                                           <  1:535326/249‑1 (MQ=255)                                       gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc                                                                                                                                                                                        >  1:109686/1‑250 (MQ=255)                                       gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc                                                                                                                                                                                        >  1:713065/1‑250 (MQ=255)                                       gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc                                                                                                                                                                                        >  1:408326/1‑250 (MQ=255)                                       gtgCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGc                                                                                                                                                                                        >  1:328430/1‑250 (MQ=255)                                             accacgaccaTTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATc                                                                                                                                                                                  <  1:499629/244‑1 (MQ=255)                                                   accaTTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAaatcga                                                                                                                                                                           >  1:103846/1‑251 (MQ=255)                                                           cagttACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGttt                                                                                                                                                                   >  1:807094/5‑251 (MQ=255)                                                             gttACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTAt                                                                                                                                                                 >  1:212776/3‑251 (MQ=255)                                                                acgagggtaCGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGc                                                                                                                                                              <  1:833517/243‑1 (MQ=255)                                                                     ggtaCGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGGCGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTg                                                                                                                                                           >  1:443813/4‑249 (MQ=255)                                                                     ggtaCGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTg                                                                                                                                                           >  1:573906/4‑249 (MQ=255)                                                                      gtaCGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATc                                                                                                                                                                                  <  1:358157/223‑1 (MQ=255)                                                                       taCGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTg                                                                                                                                                        <  1:508527/249‑1 (MQ=255)                                                                        aCGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGAc                                                                                                                                                      <  1:291446/251‑1 (MQ=255)                                                                                gggCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGgc                                                                                                                                                <  1:810904/249‑1 (MQ=255)                                                                                        tGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACTTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGATTTATGGCTTGCTGACTTTGGCGCATTACCgc                                                                                                                                      <  1:386945/251‑2 (MQ=255)                                                                                        tGTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCg                                                                                                                                       >  1:480086/1‑250 (MQ=255)                                                                                         gTTAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCgca                                                                                                                                     >  1:326311/1‑249 (MQ=255)                                                                                          ttAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCgcac                                                                                                                                    <  1:140583/251‑4 (MQ=255)                                                                                          ttAAAATTCATAGCGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCgcac                                                                                                                                    <  1:75896/251‑4 (MQ=255)                                                                                                     agcGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGttt                                                                                                                                                                   <  1:90505/207‑1 (MQ=255)                                                                                                     agcGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTgg                                                                                                                         >  1:624709/3‑251 (MQ=255)                                                                                                     agcGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTgg                                                                                                                         >  1:151873/3‑251 (MQ=255)                                                                                                       cGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGAt                                                                                                                       <  1:12318/251‑1 (MQ=255)                                                                                                       cGAACACCGACCGCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGAt                                                                                                                       <  1:831458/251‑1 (MQ=255)                                                                                                             ccgaccgCACTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGt                                                                                                                 <  1:888508/251‑1 (MQ=255)                                                                                                                      cTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGc                                                                                                                                                              <  1:203440/197‑1 (MQ=255)                                                                                                                      cTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGc                                                                                                                                                              >  1:876514/1‑197 (MQ=255)                                                                                                                      cTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCgcac                                                                                                                                    >  1:514904/1‑220 (MQ=255)                                                                                                                      cTTTTCGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTg                                                                                                        >  1:823289/1‑251 (MQ=255)                                                                                                                        tttcGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGGGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGa                                                                                                      <  1:445058/248‑1 (MQ=255)                                                                                                                         ttcGGCATTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGAt                                                                                                     <  1:477740/249‑1 (MQ=255)                                                                                                                               aTTTTGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATgg                                                                                                                                                               <  1:881742/187‑1 (MQ=255)                                                                                                                                 tttGAATTGTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACcgcg                                                                                             >  1:437937/1‑251 (MQ=255)                                                                                                                                         gTTCAGCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGAt                                                                                                     >  1:535960/1‑235 (MQ=255)                                                                                                                                              gCCCGCTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTAt                                                                                >  1:229495/1‑251 (MQ=255)                                                                                                                                                   ctACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTg                                                                            >  1:11101/2‑250 (MQ=255)                                                                                                                                                               gCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                                                                                                                                                                                           <  1:874650/127‑1 (MQ=255)                                                                                                                                                               gCGGATGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTAt                                                                                >  1:284801/1‑234 (MQ=255)                                                                                                                                                                    tGACAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCgggaaacc                                                           >  1:428935/1‑246 (MQ=255)                                                                                                                                                                      aCAAACTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATgcacc                                                                                                <  1:327108/211‑4 (MQ=255)                                                                                                                                                                         aaCTGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCgggaaaccctgttg                                                     <  1:604455/251‑11 (MQ=255)                                                                                                                                                                            tGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTgggc                                                  <  1:527982/251‑2 (MQ=255)                                                                                                                                                                            tGGAGCGCTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTgggc                                                  <  1:367894/251‑2 (MQ=255)                                                                                                                                                                                   cTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCgcac                                                                                                                                    <  1:189234/162‑4 (MQ=255)                                                                                                                                                                                   cTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTgggc                                                  <  1:644225/244‑2 (MQ=255)                                                                                                                                                                                   cTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCtgtgtgt                                           <  1:39237/251‑1 (MQ=255)                                                                                                                                                                                   cTTTATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCtgtgtg                                            <  1:420895/250‑1 (MQ=255)                                                                                                                                                                                      tATTGACGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTggg                                        <  1:278758/251‑1 (MQ=255)                                                                                                                                                                                         tgacgaATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGcgg                                     <  1:607949/247‑3 (MQ=255)                                                                                                                                                                                          gacgaATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                                                                                                                                                                                           <  1:827134/97‑1 (MQ=255)                                                                                                                                                                                          gacgaATTGTTGTTGTGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTgggctg                                                >  1:52133/4‑236 (MQ=255)                                                                                                                                                                                                 tgttgttgtGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaa                             <  1:175357/251‑2 (MQ=255)                                                                                                                                                                                                  gttgttgtGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTc                                                                                                                                                                                           <  1:3000/92‑1 (MQ=255)                                                                                                                                                                                                   ttgttgtGTGATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaag                            <  1:619874/250‑3 (MQ=255)                                                                                                                                                                                                        gtgtgATTGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaaggaggat                      >  1:406502/1‑243 (MQ=255)                                                                                                                                                                                                              ttGCGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaag                            >  1:342096/1‑237 (MQ=255)                                                                                                                                                                                                                 cGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCTTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaaggaggatttgaggat              <  1:443662/250‑17 (MQ=255)                                                                                                                                                                                                                 cGAAGATCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaaggaggatttgaggat              <  1:674652/250‑17 (MQ=255)                                                                                                                                                                                                                      aTCGTCTGCCGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGaag                            >  1:823891/1‑229 (MQ=255)                                                                                                                                                                                                                            tgccGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAAtt  <  1:819051/248‑1 (MQ=255)                                                                                                                                                                                                                             gccGCAACCGTGTTATGACGCTAACCTTGCCGAACAGATTCAGATTTCTTACAAAGAACGGATTCCGCCTAATCAAATCGAATCGGTTTATGGCTTGCTGACTTTGGCGCATTACCGCACCTCGCCGTTGGATTTACGTCGATTATTGGATGCACCGCGACAACAATTTTATTTTGCACAAACGCGGGAAACCCTGTTGGGCTGTGTGTGGGCGGTGCCTGAAGGAGGATTTGAGGATGATGCATTAAtt  >  1:408787/3‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           ATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTCAGCCCGTTACGTTGGCAGGCGGATGACAAACTGGAGCGCTTTATTGATGAATTGTTGTTGTGTGATTGCGAAGATCGTCTGACGCAACCGCGTTACGATGCCAACCTTGCCAAACAGATTCAGATTTCTTACGAAGAACAGATTCCGCCTTATCAAATCGAATCGGTTTATGGCTTGCTGACCTTGGCGCATTACCGAACCTCGCCGTTGGATTTACGTCGATTATTGGATGCGCCGCGACAACAATTTTATCTTGCGCAGACGCAGGAGGCCTTGTTGGGTTGTGTGTGGGCTGTGCCTGAGGGCGAATTTGGTGACGACGCATTAATT  >  1/887084‑887553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑