Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	144,633	A→G	S283G (AGT→GGT)	unknown →	Ribose ABC transporter, ATP‑binding protein RbsA (TC 3.A.1.2.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	144,633	0	A	G	100.0%	178.4 / NA	62	S283G (AGT→GGT)	unknown	Ribose ABC transporter, ATP‑binding protein RbsA (TC 3.A.1.2.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/27);  total (35/27)

TAATGTCACCGTGTTGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCAGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACCGCGTTAGATTATTTCGCCAAAAC  >  1/144416‑144874                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  tAATGTCACCGTGTTGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCg                                                                                                                                                                                                                  >  1:406387/1‑251 (MQ=255)     gTCACCGTGTTGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAAtt                                                                                                                                                                                                              >  1:583499/1‑251 (MQ=255)        aCCGTGTTGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGACTTAAt                                                                                                                                                                                                           >  1:324736/1‑251 (MQ=255)         ccGTGTTGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATg                                                                                                                                                                                                          <  1:105079/251‑1 (MQ=255)              ttGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGt                                                                                                                                                                                                     >  1:35245/1‑251 (MQ=255)                     aCGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGc                                                                                                                                                                                                   >  1:118870/1‑246 (MQ=255)                                                      aTTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACACTATCCGCGTCTGGAACGGGCACCCGGCGTAGCTG‑GCTTGACGGTAAACCATCTCAGTGGCAGCGGTGTACATGCTGTCTCCTTTGCATTGCGTAGCGGCGAGTTTCTCGGCATTTCCGGCTTAATGGGCACCGGTCGCGGCGAATTAATGAAAGTGCTTTACgg                                                                                                                                                                                            >  1:518831/1‑220 (MQ=255)                                                               aaaaTCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCaa                                                                                                                                                    >  1:472407/1‑251 (MQ=255)                                                                     gTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCg                                                                                                                                              >  1:769096/1‑251 (MQ=255)                                                                          aTCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGAtttt                                                                                                                                         >  1:507191/1‑251 (MQ=255)                                                                                atgatgGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCg                                                                                                                                   >  1:139769/1‑251 (MQ=255)                                                                                  gatgGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTa                                                                                                                                 >  1:842764/1‑251 (MQ=255)                                                                                   atgGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCAc                                                                                                                                                                                                                             >  1:847915/1‑158 (MQ=255)                                                                                           tcgtcgTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAgg                                                                                                                        <  1:711726/251‑1 (MQ=255)                                                                                              tcgtTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGaa                                                                                                                      <  1:28884/250‑1 (MQ=255)                                                                                              tcgtTGAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGaa                                                                                                                      <  1:527428/250‑1 (MQ=255)                                                                                               cgtTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACgg                                                                                                                                                                                            <  1:302383/179‑1 (MQ=255)                                                                                                gtTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAgg                                                                                                                    >  1:523295/1‑250 (MQ=255)                                                                                                      aagaaCAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGt                                                                                                             <  1:828138/251‑1 (MQ=255)                                                                                                        gaaCAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTaa                                                                                                           >  1:689506/1‑251 (MQ=255)                                                                                                           cAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACgg                                                                                                        >  1:108949/1‑251 (MQ=255)                                                                                                            aaTATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGc                                                                                                       <  1:801103/251‑1 (MQ=255)                                                                                                             atatCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCAc                                                                                                                                                                                                                             >  1:420936/1‑132 (MQ=255)                                                                                                              tatCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCAt                                                                                                     <  1:570492/251‑1 (MQ=255)                                                                                                                    cgTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACgg                                                                                                                                                                                            >  1:274511/1‑158 (MQ=255)                                                                                                                    cgTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGta                                                                                               >  1:161842/1‑251 (MQ=255)                                                                                                                      tCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGtat                                                                                              <  1:790733/250‑1 (MQ=255)                                                                                                                        tGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           <  1:343111/251‑1 (MQ=255)                                                                                                                        tGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           <  1:849069/251‑1 (MQ=255)                                                                                                                        tGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           <  1:437604/251‑1 (MQ=255)                                                                                                                                gcgcccGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAGAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAg                                                                                    <  1:290443/247‑1 (MQ=255)                                                                                                                                               gcgcTTGACGGTAAACCATCTCAGCGGCCGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGACTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTCATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGCGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGAGACg                                                                     >  1:855694/1‑251 (MQ=255)                                                                                                                                                  cTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATtg                                                                                                   >  1:452275/1‑218 (MQ=255)                                                                                                                                                      aCGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGtat                                                                                              >  1:139842/1‑219 (MQ=255)                                                                                                                                                      aCGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGAc                                                                                  <  1:850573/231‑1 (MQ=255)                                                                                                                                                         gTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGAtttt                                                           >  1:503562/1‑251 (MQ=255)                                                                                                                                                          tAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGAtttt                                                           <  1:558350/250‑1 (MQ=255)                                                                                                                                                                  ctcAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTc                                                  <  1:353283/251‑1 (MQ=255)                                                                                                                                                                            gcggcgTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGaa                                        >  1:307324/1‑251 (MQ=255)                                                                                                                                                                               gcgTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTaa                                                                                                           >  1:717380/1‑181 (MQ=255)                                                                                                                                                                               gcgTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           <  1:560078/197‑1 (MQ=255)                                                                                                                                                                               gcgTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           >  1:833055/1‑197 (MQ=255)                                                                                                                                                                               gcgTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAgg                                                        <  1:47731/232‑1 (MQ=255)                                                                                                                                                                               gcgTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAgg                                                        <  1:415790/232‑1 (MQ=255)                                                                                                                                                                                 gTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGt                                                                                                             <  1:686783/177‑1 (MQ=255)                                                                                                                                                                                  tACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAatat                                   <  1:263923/250‑1 (MQ=255)                                                                                                                                                                                       gatgTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           >  1:51354/1‑189 (MQ=255)                                                                                                                                                                                       gatgTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTcc                               >  1:335972/1‑249 (MQ=255)                                                                                                                                                                                       gatgTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTcc                               >  1:727388/1‑249 (MQ=255)                                                                                                                                                                                         tgTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAgg                                                                          >  1:366801/1‑204 (MQ=255)                                                                                                                                                                                              ccTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           <  1:64709/182‑1 (MQ=255)                                                                                                                                                                                              ccTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAgg                                                        >  1:229382/1‑217 (MQ=255)                                                                                                                                                                                              ccTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACcgcg                      >  1:286769/1‑251 (MQ=255)                                                                                                                                                                                              ccTTTGCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACcgcg                      >  1:353504/1‑251 (MQ=255)                                                                                                                                                                                                   gCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGaa                                                                                                                      >  1:228239/1‑150 (MQ=255)                                                                                                                                                                                                   gCATTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATAtt                                                                                           <  1:616310/177‑1 (MQ=255)                                                                                                                                                                                                     aTTGCGCAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACCGCGTTAGatt               <  1:332592/251‑1 (MQ=255)                                                                                                                                                                                                        gcgcAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGt                                                   >  1:129353/1‑212 (MQ=255)                                                                                                                                                                                                        gcgcAGCGGCGAGATTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACcg                        <  1:484769/239‑1 (MQ=255)                                                                                                                                                                                                                  gagaTTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGtata                                                                                             <  1:652006/160‑1 (MQ=255)                                                                                                                                                                                                                   agaTTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACCGCGTTAGATTATTTCGCCAAAAc  <  1:312942/250‑1 (MQ=255)                                                                                                                                                                                                                     aTTCTCGGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGAttt                                                            >  1:644521/1‑190 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  TAATGTCACCGTGTTGCGCGACGGTCAGTTTATCGGCGAAAGTGCGGTGGAAAATTTAGATGAAAATCGTCTTATCGAAATGATGGTGGGTCGTCGTTTAGAAGAACAATATCCGCGTCTGGAACGGGCACCCGGCGAAG‑TGCGCTTGACGGTAAACCATCTCAGCGGCAGCGGCGTACATGATGTCTCCTTTGCATTGCGCAGCGGCGAGATTCTCAGTATTTCCGGCTTAATGGGCACCGGTCGCAGCGAATTAATGAAAGTGCTTTACGGTGCGCTGCCGCGAGAATCCGGTGAAGTGCGGTTAAATCAACGGGCGATTTTGAATCGTACGCCACAGGAAGGCTTACGTAACGGCATTGTGTATATTTCCGAAGACCGCAAAGGCGACGGGCTGATTTTAGGTATGTCGGTCAAAGAAAATATGTCCCTTACCGCGTTAGATTATTTCGCCAAAAC  >  1/144416‑144874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑