Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	886,983	C→G	P226P (CCC→CCG)	unknown →	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	886,983	0	C	G	100.0%	84.6 / NA	30	P226P (CCC→CCG)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/11);  total (19/11)

TCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTC  >  1/886736‑887223                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 tCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGttt                                                                                                                                                                                                                                               <  1:891812/251‑1 (MQ=255)                 gTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGaa                                                                                                                                                                                                                                >  1:77978/1‑250 (MQ=255)                  tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                                                                                                                                                                                               <  1:177452/250‑1 (MQ=255)                  tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                                                                                                                                                                                               <  1:860768/250‑1 (MQ=255)                     cTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcg                                                                                                                                                                                                                           >  1:579153/1‑251 (MQ=255)                       ttCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgt                                                                                                                                                                                                                          >  1:832531/1‑249 (MQ=255)                            aactacatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaa                                                                                                                                                                                                                     <  1:536029/242‑7 (MQ=255)                                acatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaagattg                                                                                                                                                                                                                >  1:529655/5‑240 (MQ=255)                                 catcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTgg                                                                                                                                                                                                               >  1:536541/4‑251 (MQ=255)                                           ccTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATccg                                                                                                                                                                                                     >  1:450363/1‑250 (MQ=255)                                                gAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTAtat                                                                                                                                                                                                                                           >  1:418508/1‑206 (MQ=255)                                                 aaCAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCaa                                                                                                                                                                                               <  1:14588/251‑1 (MQ=255)                                                       cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                                                                                                                                                                         >  1:897422/1‑251 (MQ=255)                                                       cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                                                                                                                                                                                         >  1:272634/1‑251 (MQ=255)                                                                     cAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTAtt                                                                                                                                                                           <  1:834540/251‑1 (MQ=255)                                                                          ggcgacatccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTgg                                                                                                                                                                      >  1:68449/17‑251 (MQ=255)                                                                                 tccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgc                                                                                                                                                               <  1:278547/242‑1 (MQ=255)                                                                                  ccggtgcagagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAgcg                                                                                                                                                              >  1:443875/9‑251 (MQ=255)                                                                                          gagaTGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCgatgat                                                                                                                                                      >  1:694667/1‑251 (MQ=255)                                                                                                                                                                                                    tgAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTc                                            <  1:234747/250‑1 (MQ=255)                                                                                                                                                                                                     gAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCa                                           >  1:856567/1‑251 (MQ=255)                                                                                                                                                                                                                cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                 <  1:348820/250‑10 (MQ=255)                                                                                                                                                                                                                cGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCAtagcgaacac                                 <  1:803534/250‑10 (MQ=255)                                                                                                                                                                                                                  gTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccga                              >  1:265432/1‑251 (MQ=255)                                                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGTTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                             >  1:872472/5‑250 (MQ=255)                                                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                             >  1:777083/5‑250 (MQ=255)                                                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                             >  1:196735/5‑250 (MQ=255)                                                                                                                                                                                                                    caataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgac                             >  1:408353/5‑250 (MQ=255)                                                                                                                                                                                                                      ataTTTTCAATGAATTTGCCGCTGCGGAAATCCCGGTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGCTACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACaccgaccg                           >  1:879924/3‑250 (MQ=255)                                                                                                                                                                                                                                              cggAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTATTTGTGGATGAAGCGGCGATGATCCCGTTGGATACGCTGTTTGCTTTAACTGCCGTCTTTAAACGCATTGTGCTGACCACGACCATTCACAGTTACGAGGGTACGGGCAGGGGCTTCTTGTTAAAATTCATAGCGAACACCGACCGCACttttgttcagcgccgtcaatag  <  1:401924/249‑20 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 TCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATTTGTGGACGAAGCGGCGATGATTCCGTTGGATACGCTGTTTGATTTAACGACTGCCTTTAAACGTATTGTGCTGACGACCACCATTCACAGCTACGAAGGCACGGGCAGGGGCTTCTTGTTAAAATTCATGACGAAAACCGACCGCACTTTGCGGCATTTTGAATTGTTC  >  1/886736‑887223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑