Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	5,264	A→G	intergenic (+146/‑121)	unknown → / → unknown	Superoxide dismutase [Cu‑Zn] precursor (EC 1.15.1.1)/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	5,264	0	A	G	100.0%	93.4 / NA	35	intergenic (+146/‑121)	unknown/unknown	Superoxide dismutase [Cu‑Zn] precursor (EC 1.15.1.1)/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/16);  total (19/16)

CTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCCGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAG  >  1/5022‑5373                                                                                                                                                                                                                                                   |                                                                                                              cTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCCAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcat                                                                                                       >  1:514412/1‑251 (MQ=21)   cccTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcatca                                                                                                     >  1:555863/1‑251 (MQ=255)    ccTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAg                                                                                                    <  1:134948/251‑1 (MQ=255)           aTCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTtct                                                                                              >  1:846594/1‑250 (MQ=255)            tCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCg                                                                                            <  1:443709/251‑1 (MQ=255)                     gtggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAg                                                                                    <  1:105708/250‑1 (MQ=255)                       ggCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTtcatc                                                                                                      <  1:737475/230‑1 (MQ=18)                            tAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTAt                                                                            <  1:711632/251‑1 (MQ=255)                            tAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGCGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGCTTTCTCGTTCTCAAGTCATTTAt                                                                            >  1:566099/1‑251 (MQ=18)                                     cGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAAGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAg                                                                    >  1:438090/1‑250 (MQ=18)                                          aCCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCttt                                                               >  1:746415/1‑250 (MQ=255)                                               gCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGaa                                                         >  1:34443/1‑251 (MQ=255)                                                 cccACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACTCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAag                                                       <  1:445159/251‑1 (MQ=17)                                                   cACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAagag                                                     <  1:637682/251‑1 (MQ=255)                                                            gcggcggTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                             <  1:675978/250‑1 (MQ=255)                                                                     cgcgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAAt                                   <  1:661990/251‑1 (MQ=255)                                                                      gcgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAAt                                             <  1:303672/240‑1 (MQ=14)                                                                       cgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTaaaa                                    >  1:226559/1‑248 (MQ=255)                                                                       cgTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATc                                  >  1:624914/1‑250 (MQ=255)                                                                            ggCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTta                            >  1:683248/1‑251 (MQ=255)                                                                            ggCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTta                            >  1:755968/1‑251 (MQ=255)                                                                            ggCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTta                            >  1:870789/1‑251 (MQ=255)                                                                               gtgtGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATgg                                           >  1:613573/1‑233 (MQ=14)                                                                               gtgtGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                         >  1:429341/1‑251 (MQ=255)                                                                                   ggtgTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATaaa                      >  1:499357/1‑250 (MQ=255)                                                                                    gtgtAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTg                                                                          >  1:296642/1‑197 (MQ=14)                                                                                    gtgtAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTtatat                         >  1:194923/1‑246 (MQ=255)                                                                                          ttAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAAc              <  1:264823/251‑1 (MQ=255)                                                                                           tAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACg             <  1:189453/251‑1 (MQ=255)                                                                                            aaaTAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACg             >  1:377928/1‑250 (MQ=255)                                                                                              ataatCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGcc           >  1:4314/1‑250 (MQ=255)                                                                                                    cGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt    <  1:456693/251‑1 (MQ=255)                                                                                                    cGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAAt    <  1:768512/251‑1 (MQ=255)                                                                                                      cTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg  <  1:731158/251‑1 (MQ=255)                                                                                                      cTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCTGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTTAGAAATTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTGAACTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAg  <  1:327616/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                              CTCCCTCATGATCCACGCTGGTGGCGATAACCACTCCGATCACCCGGCCCCACTTGGCGGCGGCGGTCCGCGTATGGCGTGTGGTGTAATTAAATAATCCGCTTTCCGAAACAAAAAAGTGATCTGCACCCCAAAACCCGGACACCTAATTTGAGGTGCAGATTATGTCTTACTCTTATCAATTTCGTTAAAAAACTATCAAACAGGTCACCGAGCAGGATTTTGGTATCCGTGAGGTGGCTAAATTTCATCAGATTTCTCGTTCTCAAGTCATTTATTGGAAAAGCCTTTCGTGAAAGAGGGCTTAATGGCGTAAAATCCCCTTATATAAACCCTCAACGCCCTAAAATAG  >  1/5022‑5373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑