Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	886,824	G→A	A173A (GCG→GCA)	unknown →	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	886,824	0	G	A	100.0%	166.0 / NA	58	A173A (GCG→GCA)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/33);  total (25/33)

GATTTGGCGGCACAACCCGACGGCGACAGCCTGCGTTGGAGCAGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCGGAAATACAGCTTCCCCATTTATCAACATTTGCCGTCTGCGCTCGTTTTCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATT  >  1/886588‑887054                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       gATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATc                                                                                                                                                                                                                            <  1:707442/249‑1 (MQ=255) gATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGt                                                                                                                                                                                                                          >  1:780503/1‑251 (MQ=255)               acctgatggtgACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAAt                                                                                                                                                                                                                             >  1:539034/11‑234 (MQ=255)                             gcttGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATc                                                                                                                                                                                               >  1:639360/4‑250 (MQ=255)                              cttGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATc                                                                                                                                                                                               <  1:241184/247‑1 (MQ=255)                              cttGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATc                                                                                                                                                                                               <  1:296060/247‑1 (MQ=255)                                 gCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAAt                                                                                                                                                                                                                             <  1:171207/216‑1 (MQ=255)                                  cGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCtt                                                                                                                                                                                          <  1:478547/250‑1 (MQ=255)                                     tGGAGCGGTGAAAAACACCCGATAAACACACCGCTCTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGc                                                                                                                                                                                      <  1:582660/251‑1 (MQ=255)                                          cggTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGAGTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCt                                                                                                                                                                                 <  1:718452/249‑1 (MQ=255)                                               aaaaaCACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTc                                                                                                                                                                            <  1:104312/251‑1 (MQ=255)                                                      cccGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCaag                                                                                                                                                                      >  1:518565/1‑249 (MQ=255)                                                         gATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTTAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAg                                                                                                                                                                   >  1:463983/1‑250 (MQ=255)                                                          aTAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAagcagc                                                                                                                                                                  <  1:669075/250‑1 (MQ=255)                                                            aaaCACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAAt                                                                                                                                                               >  1:738563/1‑251 (MQ=255)                                                                                 cTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTc                                                                                                                                          <  1:515335/251‑1 (MQ=255)                                                                                 cTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTc                                                                                                                                          <  1:204357/251‑1 (MQ=255)                                                                                      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     <  1:471226/251‑1 (MQ=255)                                                                                      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     <  1:578806/251‑1 (MQ=255)                                                                                      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     <  1:65902/251‑1 (MQ=255)                                                                                      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     <  1:660618/251‑1 (MQ=255)                                                                                      tGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     <  1:396196/251‑1 (MQ=255)                                                                                       gCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGa                                                                                                                                    >  1:324372/1‑251 (MQ=255)                                                                                                                   cccATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     >  1:782676/1‑222 (MQ=255)                                                                                                                    ccATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGc                                                                                                                                                                                                                    <  1:31774/142‑1 (MQ=255)                                                                                                                      aTTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTAt                                                                                                                                       <  1:89370/217‑1 (MQ=255)                                                                                                                      aTTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATAtttt                                                                                                     <  1:128232/251‑1 (MQ=255)                                                                                                                      aTTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATAtttt                                                                                                     <  1:246325/251‑1 (MQ=255)                                                                                                                       tttATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTc                                                                                                    <  1:116385/251‑1 (MQ=255)                                                                                                                        ttATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCa                                                                                                   >  1:795066/1‑251 (MQ=255)                                                                                                                          aTCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAAt                                                                                                                                                                                                                             >  1:295264/1‑127 (MQ=255)                                                                                                                          aTCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACgg                                                                                                                                 >  1:629920/1‑219 (MQ=255)                                                                                                                                 tctgccgcccgcgcTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAAtttg                                                                                          >  1:791685/11‑250 (MQ=255)                                                                                                                                           gcgcTGGTCTTCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCgctgcgga                                                                                >  1:853880/1‑244 (MQ=255)                                                                                                                                                 gtcttCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATcc                                                                           <  1:641658/247‑1 (MQ=255)                                                                                                                                                     tCCCTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGttt                                                                      <  1:891812/251‑1 (MQ=255)                                                                                                                                                      cccTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAAttggaagcaaatcagcc                                                                                                                                               <  1:48300/177‑16 (MQ=255)                                                                                                                                                      cccTAGGATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     >  1:471769/1‑187 (MQ=255)                                                                                                                                                           ggATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAAttggaagc                                                                                                                                                        <  1:335158/162‑7 (MQ=255)                                                                                                                                                           ggATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGAc                                                                                                                                   <  1:613195/183‑1 (MQ=255)                                                                                                                                                           ggATTGAAACGTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATcc                                                                           <  1:837591/239‑1 (MQ=255)                                                                                                                                                                     gTCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGaa                                                       >  1:77978/1‑250 (MQ=255)                                                                                                                                                                      tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                      <  1:860768/250‑1 (MQ=255)                                                                                                                                                                      tCGCTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAAc                                                      <  1:177452/250‑1 (MQ=255)                                                                                                                                                                         cTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcg                                                  >  1:579153/1‑251 (MQ=255)                                                                                                                                                                         cTTTCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGACAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     >  1:71132/1‑168 (MQ=255)                                                                                                                                                                           ttCAGAACTACATCAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgt                                                 >  1:832531/1‑249 (MQ=255)                                                                                                                                                                                aactacatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaa                                            <  1:536029/242‑7 (MQ=255)                                                                                                                                                                                    acatcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTgcgtcgaaagattg                                       >  1:529655/5‑240 (MQ=255)                                                                                                                                                                                     catcAACCGACCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTgg                                      >  1:536541/4‑251 (MQ=255)                                                                                                                                                                                              aCCTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTg                                                                                                                                     >  1:645381/1‑147 (MQ=255)                                                                                                                                                                                               ccTTGGAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATccg                            >  1:450363/1‑250 (MQ=255)                                                                                                                                                                                                    gAACAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTAtat                                                                  >  1:418508/1‑206 (MQ=255)                                                                                                                                                                                                     aaCAAACCCATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCaa                      <  1:14588/251‑1 (MQ=255)                                                                                                                                                                                                         aacccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAg                                                                                                                    <  1:724398/151‑1 (MQ=255)                                                                                                                                                                                                           cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                >  1:272634/1‑251 (MQ=255)                                                                                                                                                                                                           cccATTTATTGCGGCAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCg                >  1:897422/1‑251 (MQ=255)                                                                                                                                                                                                                         cAAATGGCGACATCCGGTGCAGAGATGTTAATCGTTACGGCAAAGCGTGGGCGGGGCAAATCCGCTTTGGCAGGCTTGTTCGCCAAGCAGCAATTGGAAGCAAATCAGCCTGTTCTATTGACGGCGCTGAACAAAAGTGCGGTCAATATTTTCAATGAATTTGCCGCTGCGGAAATCCCGTTTATATCGCCTGATGAACTGCGTCGAAAGATTGGTGAAAATCCGCAGCAATTTGCGGATTTATGGTTAtt  <  1:834540/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       GATTTGGCGGCACAACCCGACGGCGACAGCCTGCGTTGGAGCAGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCGGAAATACAGCTTCCCCATTTATCAACATTTGCCGTCTGCGCTCGTTTTCCCTAAGATTGAAAAGTCGCTTTCAGAACAACACCAACCGACCTTGGAACAAGCCCATTTATTGCGGCAAATGGCGACATCTGACGCGGAGATGTTAATCGTTACGGCAAAGCGCGGGCGGGGAAAATCCGCTTTGGCGGGCTTGTTCGCCAAACAGCAATTAGCGGCAAATAAGCCCGTTCTATTGACGGCGCCGAACAAAAGTGCGGTCAAGATTTTCAATGAATTTTCCGGTGTCGAAATCCCCTTTATGCCACCTGATGAACTGCGCCAAAAGGTTGGTGAAAATCCACAGCAATTTGCGGATTCGTGGTTATT  >  1/886588‑887054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑