Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	886,521	C→T	A72A (GCC→GCT)	unknown →	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	886,521	0	C	T	100.0%	203.3 / NA	69	A72A (GCC→GCT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/24);  total (45/24)

GATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAAGGCATTCATTTAGATGCCCTCGCTATTGCCGCCGGTGCGTTGTCGGAAGGCGGCACATTGTTGCTGTTATTCAATGAGTGGCAGGATTTGGCGGCACAACCCGACGGCGACAGCCTGCGTTGGAGCAGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCGGAAATACAGCTTCCCCATTTATCAACATTTGCCGTCTGCGCTCGTTTTCCCTAAGATTGAAAAGTCGCTTTCA  >  1/886279‑886761                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gatCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGccat                                                                                                                                                                                                                                          >  1:238826/1‑248 (MQ=255)   tCCGTATTCACGGGGTTGGTAAACTATGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTg                                                                                                                                                                                                                                        >  1:179552/1‑251 (MQ=255)                       aaactaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGaa                                                                                                                                                                                                                    <  1:248402/246‑1 (MQ=255)                         actaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGcc                                                                                                                                                                                                                                            >  1:592869/4‑224 (MQ=255)                          ctaTGGCGCGGCAGTTAATTCTTCTCATTGGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCAGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAgg                                                                                                                                                                                                                  >  1:212219/3‑250 (MQ=255)                                     cagttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                                                                                                                                       <  1:300262/234‑1 (MQ=255)                                     cagttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                                                                                                                                       <  1:193059/234‑1 (MQ=255)                                      agttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgttg                                                                                                                                                                                                     >  1:870287/16‑251 (MQ=255)                                      agttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgttg                                                                                                                                                                                                     >  1:470535/16‑251 (MQ=255)                                       gttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                                                                                                                                       <  1:271834/234‑1 (MQ=255)                                        ttaattcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgttg                                                                                                                                                                                                     <  1:56150/236‑1 (MQ=255)                                             tcttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTAt                                                                                                                                                                                              >  1:726572/9‑251 (MQ=255)                                               ttctcattgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTc                                                                                                                                                                                            <  1:104009/245‑1 (MQ=255)                                                    attgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGa                                                                                                                                                                                       <  1:867890/250‑1 (MQ=255)                                                     ttgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGat                                                                                                                                                                                      >  1:307769/1‑250 (MQ=255)                                                     ttgGTGACGATGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGa                                                                                                                                                                                       <  1:14211/250‑1 (MQ=255)                                                       ggtgacgaTGCCACGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATAttgt                                                                                                                                                                                                       >  1:411686/4‑232 (MQ=255)                                                                   aCGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggc                                                                                                                                                                        <  1:29227/251‑1 (MQ=255)                                                                   aCGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTgg                                                                                                                                                                         >  1:59991/1‑250 (MQ=255)                                                                   aCGTTAGCGCACCAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTgg                                                                                                                                                                         >  1:630816/1‑250 (MQ=255)                                                                              ccAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaac                                                                                                                                                                >  1:756796/1‑242 (MQ=255)                                                                              ccAATGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaac                                                                                                                                                                >  1:2743/1‑242 (MQ=255)                                                                                  tGGGAACATTTTCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatgg                                                                                                                                                         <  1:63206/251‑14 (MQ=255)                                                                                   gggaacattttCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatggg                                                                                                                                                        >  1:253168/9‑237 (MQ=255)                                                                                       acattttCCTGCCCTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatggtgaca                                                                                                                                                    >  1:309866/5‑233 (MQ=255)                                                                                                  cccTGAAAAGTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGt                                                                                                                                                                                                                         >  1:71496/1‑171 (MQ=255)                                                                                                           gTGCGGTATGGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTGTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacc                                                                                                                                                               <  1:893088/220‑8 (MQ=255)                                                                                                                gtgaaacgccgccaatccataccGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGc                                                                                                                                      >  1:335772/22‑240 (MQ=255)                                                                                                                gtatgGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAaca                                                                                                                           >  1:235089/4‑251 (MQ=255)                                                                                                                  atgGATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGa                                                                                                                                                                                                                     >  1:287215/2‑159 (MQ=255)                                                                                                                    ggATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCg                                                                                                                       >  1:212676/1‑251 (MQ=255)                                                                                                                     gATTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGa                                                                                                                      >  1:210987/1‑251 (MQ=255)                                                                                                                      aTTGGCGGCGTTTCACCGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGAt                                                                                                                     <  1:642971/251‑1 (MQ=255)                                                                                                                           cggcgtttcaccGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAac                                                                                                                 <  1:719897/240‑1 (MQ=255)                                                                                                                             gcgtttcaccGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAac                                                                                                                 >  1:71257/9‑248 (MQ=255)                                                                                                                              cgtttcaccGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAacacac                                                                                                             <  1:409167/244‑1 (MQ=255)                                                                                                                                  tcaccGCACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCaa                                                                                                                                                                                          >  1:382846/4‑170 (MQ=255)                                                                                                                                        cACTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTAt                                                                                                   <  1:37136/251‑1 (MQ=255)                                                                                                                                          cTTTTCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTg                                                                                                 >  1:436916/1‑251 (MQ=255)                                                                                                                                           ttttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                <  1:25093/251‑1 (MQ=255)                                                                                                                                           ttttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                <  1:322993/251‑1 (MQ=255)                                                                                                                                            tttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGATCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                >  1:546230/1‑250 (MQ=255)                                                                                                                                            tttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGcc                                                                                               <  1:163458/251‑1 (MQ=255)                                                                                                                                            tttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                >  1:151475/1‑250 (MQ=255)                                                                                                                                            tttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                >  1:552108/1‑250 (MQ=255)                                                                                                                                            tttCTGCTCAGCCTCACTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                >  1:239994/1‑250 (MQ=255)                                                                                                                                                                                aaaaTCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTggcggtgcaacctgatgg                                                                                                                                                         <  1:485204/157‑14 (MQ=255)                                                                                                                                                                                  aaTCTGCTCGGCAGCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCAt                                                         <  1:36715/251‑1 (MQ=255)                                                                                                                                                                                               gCGAATTCCCCGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCaatat                                               >  1:473493/1‑245 (MQ=255)                                                                                                                                                                                                      ccccGCGCTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCgcccg                                     >  1:802091/1‑247 (MQ=255)                                                                                                                                                                                                          gcgcTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGc                                                                                                >  1:335624/1‑188 (MQ=255)                                                                                                                                                                                                          gcgcTTATTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCgcccgcg                                   >  1:591353/1‑243 (MQ=255)                                                                                                                                                                                                               taTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGTCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCttgt                              >  1:582163/2‑246 (MQ=255)                                                                                                                                                                                                               taTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCtggt                              >  1:51859/2‑246 (MQ=255)                                                                                                                                                                                                               taTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCtggt                              >  1:734236/2‑246 (MQ=255)                                                                                                                                                                                                               taTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCtggt                              >  1:466534/2‑246 (MQ=255)                                                                                                                                                                                                               taTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCtggt                              >  1:467746/2‑246 (MQ=255)                                                                                                                                                                                                               taTTTATGATGGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCtggt                              >  1:545766/2‑246 (MQ=255)                                                                                                                                                                                                                       atgGGCGTCAGGGCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTag                     <  1:703132/250‑2 (MQ=255)                                                                                                                                                                                                                           gcgtcagggCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTg                >  1:7940/8‑251 (MQ=255)                                                                                                                                                                                                                                 gggCATTCATTTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGaaacgt          >  1:722041/2‑248 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTAt                                                                                                   <  1:782028/155‑1 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGcc                                                                                               <  1:116375/159‑1 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCttt    >  1:373382/1‑250 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCttt    >  1:726986/1‑250 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCttt    >  1:132686/1‑250 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGCCTTCCCTAGGATTGaaa             >  1:774487/1‑241 (MQ=255)                                                                                                                                                                                                                                        cattTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTCGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCtggtc                             <  1:424225/225‑5 (MQ=255)                                                                                                                                                                                                                                         attTAGATGCTCTCGCCATTGCCGCCGGTGCGCTGTCGGAAGGCGGCATATTGTTGCTGTTATTCAATGATTGGCAGGATTTGGCGGTGCAACCTGATGGTGACAGCTTGCGTTGGAGCGGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCAAAAATACGGCTTTCCCATTTATCAATATCTGCCGCCCGCGCTGGTCTTCCCTAGGATTGAAACGTCGCTTTCa  >  1:7795/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GATCCGTATTCGCGGGGTTGGTAAAGCATGGCGCGGCAGCTAATTCTCCTTGTTGGCGACGATGCCACGTTAGCGCGCCAATGGGAATACTTTCCTGCCCTGAAAAGTGCGGTGTGGATTGGCGGTGTTTTGCCGCACTTTTCCGCTCAACTTCATTTCCCTTTTAGCAAAAGTAAAAATCTGCTCGGCAGCGAATTCCCCGCGCTCATTTATGATGGGCGCCAAGGCATTCATTTAGATGCCCTCGCTATTGCCGCCGGTGCGTTGTCGGAAGGCGGCACATTGTTGCTGTTATTCAATGAGTGGCAGGATTTGGCGGCACAACCCGACGGCGACAGCCTGCGTTGGAGCAGTGAAAAACACCCGATAAACACACCGCACTTTATTGCCTTTCTGCAAGAAAAAATCCGGAAATACAGCTTCCCCATTTATCAACATTTGCCGTCTGCGCTCGTTTTCCCTAAGATTGAAAAGTCGCTTTCA  >  1/886279‑886761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑