Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	885,500	C→T	intergenic (+66/‑101)	unknown → / → unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	885,500	0	C	T	100.0%	186.3 / NA	67	intergenic (+66/‑101)	unknown/unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/33);  total (34/33)

AGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTT  >  1/885257‑885737                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ggctcaagagcaagagaaacCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTa                                                                                                                                                                                                                                         >  1:148120‑M1/5‑250 (MQ=255)  gctcaagagcaagagaaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaa                                                                                                                                                                                                                                        >  1:606047‑M1/4‑249 (MQ=255)           cAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat                                                                                                                                                                                                                               >  1:653899‑M1/1‑240 (MQ=255)            aaGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatata                                                                                                                                                                                                                              <  1:139343‑M1/251‑13 (MQ=255)                 aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                                                                                                                                                          <  1:591276‑M1/250‑17 (MQ=255)                 aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatgg                                                                                                                                                                                                                           <  1:411858‑M1/249‑16 (MQ=255)                                    gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggctttt                                                                                                                                                                                                      >  1:530022‑M1/1‑215 (MQ=255)                                    gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                                                                                                                                                          >  1:781562‑M1/1‑215 (MQ=255)                                         cacaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggt                                                                                                                                                                                                 >  1:115923‑M1/1‑210 (MQ=255)                                         cacaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggt                                                                                                                                                                                                 >  1:797600‑M1/1‑210 (MQ=255)                                          acaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggct                                                                                                                                                                                                         <  1:246222‑M1/242‑34 (MQ=255)                                           caTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                                                                                                                                                          >  1:637499‑M1/1‑208 (MQ=255)                                             tggtggTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat                                                                                                                                                                                                                               >  1:192644‑M1/1‑206 (MQ=255)                                               gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaag                                                                                                                                                                                                   >  1:897391‑M1/1‑204 (MQ=255)                                               gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatat                                                                                                                                                                                                                             >  1:348005‑M1/1‑204 (MQ=255)                                               gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat                                                                                                                                                                                                                               <  1:364300‑M1/215‑12 (MQ=255)                                                         gcagtttGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttataccgtaattcaggcttttaaggttgctgcagcgggac                                                                                                                                                                                  <  1:618874‑M1/247‑57 (MQ=255)                                                            gTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatat                                                                                                                                                                                                                             >  1:497351‑M1/1‑191 (MQ=255)                                                              ttGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgatt                                                                                                                                                                            <  1:845801‑M1/251‑63 (MQ=255)                                                                gCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatac                                                                                                                                                                                                                     >  1:482943‑M1/1‑187 (MQ=255)                                                                  aTTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatc                                                                                                                                                                        <  1:761325‑M1/251‑67 (MQ=255)                                                                      aCTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctatt                                                                                                                                                                    >  1:66273‑M1/1‑181 (MQ=255)                                                                         cGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcacggaagggaccggat                                                                                                                                                                           >  1:333148‑M1/1‑178 (MQ=255)                                                                          ggTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttca                                                                                                                                                                >  1:472576‑M1/1‑177 (MQ=255)                                                                           gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatc                                                                                                                                                                        <  1:855640‑M1/242‑67 (MQ=255)                                                                           gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgat                                                                                                                                                                             >  1:150714‑M1/1‑176 (MQ=255)                                                                           gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgc                                                                                                                                                                                           <  1:127641‑M1/223‑48 (MQ=255)                                                                           gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcagg                                                                                                                                                                                                           >  1:610940‑M1/1‑176 (MQ=255)                                                                           gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatgg                                                                                                                                                                                                                           >  1:621619‑M1/1‑176 (MQ=255)                                                                                gATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctga                                                                                                                                                          >  1:471959‑M1/1‑171 (MQ=255)                                                                                 aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaa                                                                                                                                                         >  1:446602‑M1/1‑170 (MQ=255)                                                                                 aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgt                                                                                                                                                                                                                   <  1:349224‑M1/193‑24 (MQ=255)                                                                                    atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaagga                                                                                                                                                      >  1:690648‑M1/1‑167 (MQ=255)                                                                                    atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaagga                                                                                                                                                      >  1:722156‑M1/1‑167 (MQ=255)                                                                                      atTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgt                                                                                                                                                                                                                   >  1:181142‑M1/1‑165 (MQ=255)                                                                                      atTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatat                                                                                                                                                                                                                             <  1:336420‑M1/178‑14 (MQ=255)                                                                                       ttGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaata                                                                                                                                                   <  1:426788‑M1/251‑88 (MQ=255)                                                                                         gAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                                                                                                                                                          >  1:780432‑M1/1‑162 (MQ=255)                                                                                             aTGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgt                                                                                                                                             <  1:842769‑M1/251‑94 (MQ=255)                                                                                              tGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatat                                                                                                                                                  >  1:204574‑M1/1‑157 (MQ=255)                                                                                                         aggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatac                                                                                                                                  <  1:702070‑M1/250‑105 (MQ=255)                                                                                                          ggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatat                                                                                                                                                  <  1:333155‑M1/233‑89 (MQ=255)                                                                                                            aaaTGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttac                                                                                                                              <  1:187543‑M1/251‑109 (MQ=255)                                                                                                                                  aaTTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgt                                                                                                                                                                                                                   >  1:658073‑M1/1‑121 (MQ=255)                                                                                                                                    ttATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaagg                                                                                                                                                                                                  >  1:366629‑M1/1‑119 (MQ=255)                                                                                                                                    ttATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                                                                                                                                                          >  1:383742‑M1/1‑119 (MQ=255)                                                                                                                                                    cttTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATAATTAATCTATTTCCACCCTGAAGAAATATCATGTAAAAAAAATCCTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGtt                                                                                  <  1:189979/250‑1 (MQ=255)                                                                                                                                                          cGATCCGTATCTGAGCGAGAAGTAAAAAAAAGTCGAAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAACCGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTTTTTTCAACCTGAAGGAATATCATGTAAAAAAAATACTTATTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                           <  1:386685/251‑1 (MQ=255)                                                                                                                                                           gATCCGTATCTGAGCGAGAAGTAAGAAAAAGGCGGAAAGTGTAAAACTATTTCGAAAAAACAGGGTCAAACGGGCTTTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                           <  1:43973/250‑1 (MQ=255)                                                                                                                                                            aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaccctgaaggaatatcatgttaaaaaaatacttacttattttaccgatttctattttaaccgcctgtgccggttattatc                                                                                 >  1:444596‑M1/1‑95 (MQ=255)                                                                                                                                                            aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttacttattttcccgatttctattttaaccgcctgtgccggtt                                                                                       <  1:580545‑M1/242‑148 (MQ=255)                                                                                                                                                            aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCt                                                                                                                                                                  <  1:460939/162‑1 (MQ=255)                                                                                                                                                            aTCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAgg                                                                                                                                                  <  1:391927/178‑1 (MQ=255)                                                                                                                                                                gTATCTGAGCGAGAAGTAAGAAAAAGTCGGAGAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCg                                                                                                              >  1:42256/1‑210 (MQ=255)                                                                                                                                                                 tATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttacttattttaccgatttctattttaaccgcctgtgccggttattatccttatccg                                                                         >  1:711569‑M1/1‑90 (MQ=255)                                                                                                                                                                             ggagatgtgtataagaaaaattgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCA‑‑TA‑A‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAAAAATGATTAATCTTTTTTCAACCTGAAGGAATATCATTTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAAcc                                                         <  1:522820/229‑1 (MQ=255)                                                                                                                                                                                     aaaaGTCGGAAAGTGAAAAACTATTTCGGAAAACCAGGGTAAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATTCAGGCTTTTAAGTTTGCTGCAGCGGGAAAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTTCTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATcc                                                <  1:223172/251‑1 (MQ=255)                                                                                                                                                                                             gaaagtgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAat                                                                                                                                               <  1:424091/144‑1 (MQ=255)                                                                                                                                                                                                 gtgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttacttattttaccgatttctattttaaccgcctgtgccggttattatccttatccgcaatttgaacctggttatccggtcc                                                <  1:396556‑M1/244‑187 (MQ=255)                                                                                                                                                                                                 gtgtAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttacttattttaccgatttctattttaac                                                                                                     <  1:771184‑M1/191‑134 (MQ=255)                                                                                                                                                                                                    tAAAACTATATCGAAAAAACAGGGCCAACGGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATCCAGGTTTTTAGGGTGGCGCCAGCGGAACAATGATTATTCTATTTTCACCCTAAAGGAATATCATTTTAAAAAAATACTTGTTTATTTTACCGTTTTGTATTTAAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCC‑ggtcccttccgtgc                                 <  1:508630/250‑14 (MQ=255)                                                                                                                                                                                                             ttCGGAAAAACAGGTTAAAACGGGAATTCTTGCTTCATTTGCCAT‑‑‑TA‑‑AAAGCCATATATGTTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCAACCTAAAGGAATATCATGTAAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       <  1:810714/251‑1 (MQ=255)                                                                                                                                                                                                             ttCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGTTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTCCCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       <  1:710923/251‑1 (MQ=255)                                                                                                                                                                                                                 gaaaaacggggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGAACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTTTTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGa                       <  1:143318/239‑1 (MQ=255)                                                                                                                                                                                                                         gggTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCACCCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTAttatcc                                                                           <  1:330856/188‑1 (MQ=255)                                                                                                                                                                                                                           gTCAAACGGGCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATg         >  1:327642/1‑251 (MQ=255)                                                                                                                                                                                                                                  gggCATTCTTGCTTCAGTTGCCAT‑‑‑TA‑‑AAAGCCATATATGGTTATACGTAATTCAGGCTTTTAAGGTTGCTGCAGCGGGACAATGATTAATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTATTTTACCGATTTCTATTTTAACCGCCTGTGCCGGTTATTATCCTTATCCGCAATTTGAACCTGGTTATCCGGTCC‑CTTCCGTGCAGCATAATGATGATGAAGAAAATGCCGtttt  >  1:153639/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               AGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATACTTACTTGTTTTACCGATTTCTATTTTAACCGCCTGCGCGGGTTATTATCCTTATCCGCAATTTGAACCCAGTTATCC‑GTCCTTTTCCGTGCAGCATAATGACGATGAAGAACATGCCGTTTT  >  1/885257‑885737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑