Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	885,380	G→A	E137E (GAG→GAA)	unknown →	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	885,380	0	G	A	100.0%	237.2 / NA	82	E137E (GAG→GAA)	unknown	Metal‑dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/38);  total (44/38)

CTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGTCGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATA  >  1/885135‑885614                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           ctcaaTTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCa                                                                                                                                                                                                                                       >  1:390134/4‑251 (MQ=255)          cGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGGAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                                                                                                              <  1:3532/251‑1 (MQ=255)             ggCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAgg                                                                                                                                                                                                                           >  1:576486/1‑251 (MQ=255)                        ccccACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTg                                                                                                                                                                                                                >  1:59905/1‑251 (MQ=255)                          ccACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCACATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGt                                                                                                                                                                                                               >  1:85136/1‑250 (MQ=255)                          ccACTAATGTGCTGTCTTTCCCTTTTGAATCCCCTGATGAAGTGGAACTGCCGTTATTGGGCAATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTc                                                                                                                                                                                                              <  1:350772/251‑1 (MQ=255)                                     cTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATc                                                                                                                                                                                                    <  1:897916/250‑1 (MQ=255)                                     cTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATc                                                                                                                                                                                                    <  1:210376/250‑1 (MQ=255)                                       gTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGa                                                                                                                                                                                                 >  1:630872/1‑251 (MQ=255)                                       gTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGa                                                                                                                                                                                                 >  1:40111/1‑251 (MQ=255)                                       gTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGa                                                                                                                                                                                                 >  1:521505/1‑251 (MQ=255)                                        tCTTTCCCTTTTGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAg                                                                                                                                                                                                <  1:588628/251‑1 (MQ=255)                                                   tGAATGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCt                                                                                                                                                                                                   >  1:793496/1‑237 (MQ=255)                                                      aTGCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGaaa                                                                                                                                                                                  <  1:865091/251‑1 (MQ=255)                                                        gCCCTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGggcttggc                                                                                                                                                                                                                    <  1:302764/215‑1 (MQ=255)                                                           cTGATGAAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                                                                                                              <  1:62584/202‑1 (MQ=255)                                                             gatgaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCgg                                                                                                                                                                           >  1:867279/1‑251 (MQ=255)                                                             gatgaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCgg                                                                                                                                                                           >  1:354887/1‑251 (MQ=255)                                                             gaggaAGTGGAACTGCCGTTATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATc                                                                                                                                                                                                          <  1:211345/217‑1 (MQ=255)                                                                         ctgccgttATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAAc                                                                                                                                                               <  1:200994/244‑1 (MQ=255)                                                                            ccgttATTGGGCGATTTGGTGATTTGTCGTCAAGTGGTGGAGAGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCAGTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTa                                                                                                                                                             <  1:433959/246‑1 (MQ=255)                                                                                                gATTTGTCGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGaaag                                                                                                                                                                       <  1:28042/220‑3 (MQ=255)                                                                                                     gtcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                                                                                                              >  1:887233/3‑160 (MQ=255)                                                                                                     gtcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACggg                                                                                                                                    >  1:542021/3‑250 (MQ=255)                                                                                                      tcGTCAAGTGGTGGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCa                                                                                                                                  <  1:9290/250‑1 (MQ=255)                                                                                                             gtggtgGAGCGCGAGGCTCAAGAGCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTg                                                                                                                            >  1:143813/1‑250 (MQ=255)                                                                                                                           ggctcaagagcaagagaaacCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTa                                                                                                             >  1:148120‑M1/5‑250 (MQ=255)                                                                                                                            gctcaagagcaagagaaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaa                                                                                                            >  1:606047‑M1/4‑249 (MQ=255)                                                                                                                            gctcaagagcaagagAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGaa                                                                                                                                                     <  1:808703/207‑1 (MQ=255)                                                                                                                                  gagCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACg                                                                                                                                      <  1:353159/219‑1 (MQ=255)                                                                                                                                  gagCAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGttg                                                                                                                   <  1:817617/238‑3 (MQ=255)                                                                                                                                     cAAGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat                                                                                                   >  1:653899‑M1/1‑240 (MQ=255)                                                                                                                                      aaGAGAAACCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatata                                                                                                  <  1:139343‑M1/251‑13 (MQ=255)                                                                                                                                           aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                              <  1:591276‑M1/250‑17 (MQ=255)                                                                                                                                           aaaCCGCTCATGGCGCATTGGGCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatgg                                                                                               <  1:411858‑M1/249‑16 (MQ=255)                                                                                                                                                              gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGttg                                                                                                                   <  1:645615/210‑3 (MQ=255)                                                                                                                                                              gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggctttt                                                                          >  1:530022‑M1/1‑215 (MQ=255)                                                                                                                                                              gggCACACATGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                              >  1:781562‑M1/1‑215 (MQ=255)                                                                                                                                                                   cacaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggt                                                                     >  1:115923‑M1/1‑210 (MQ=255)                                                                                                                                                                   cacaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggt                                                                     >  1:797600‑M1/1‑210 (MQ=255)                                                                                                                                                                    acaTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggct                                                                             <  1:246222‑M1/242‑34 (MQ=255)                                                                                                                                                                     caTGGTGGTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                              >  1:637499‑M1/1‑208 (MQ=255)                                                                                                                                                                       tggtggTGCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat                                                                                                   >  1:192644‑M1/1‑206 (MQ=255)                                                                                                                                                                         gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAg                                                                                                                                               >  1:169208/1‑171 (MQ=255)                                                                                                                                                                         gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCt                                                                                                                          <  1:236684/192‑1 (MQ=255)                                                                                                                                                                         gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaag                                                                       >  1:897391‑M1/1‑204 (MQ=255)                                                                                                                                                                         gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatat                                                                                                 >  1:348005‑M1/1‑204 (MQ=255)                                                                                                                                                                         gtggtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatat                                                                                                   <  1:364300‑M1/215‑12 (MQ=255)                                                                                                                                                                            gtgCACGGCAGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAg                                                                                                                                                                                                                            >  1:770927/1‑91 (MQ=255)                                                                                                                                                                                   gcagtttGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttataccgtaattcaggcttttaaggttgctgcagcgggac                                                      <  1:618874‑M1/247‑57 (MQ=255)                                                                                                                                                                                      gTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatat                                                                                                 >  1:497351‑M1/1‑191 (MQ=255)                                                                                                                                                                                        ttGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgatt                                                <  1:845801‑M1/251‑63 (MQ=255)                                                                                                                                                                                          gCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatac                                                                                         >  1:482943‑M1/1‑187 (MQ=255)                                                                                                                                                                                            aTTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatc                                            <  1:761325‑M1/251‑67 (MQ=255)                                                                                                                                                                                              ttACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATc                                                                                                                                                                                                    <  1:5396/97‑1 (MQ=255)                                                                                                                                                                                                aCTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctatt                                        >  1:66273‑M1/1‑181 (MQ=255)                                                                                                                                                                                                   cGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcacggaagggaccggat                                               >  1:333148‑M1/1‑178 (MQ=255)                                                                                                                                                                                                    ggTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttca                                    >  1:472576‑M1/1‑177 (MQ=255)                                                                                                                                                                                                     gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                                                                                                              >  1:732205/1‑64 (MQ=255)                                                                                                                                                                                                     gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatc                                            <  1:855640‑M1/242‑67 (MQ=255)                                                                                                                                                                                                     gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgat                                                 >  1:150714‑M1/1‑176 (MQ=255)                                                                                                                                                                                                     gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgc                                                               <  1:127641‑M1/223‑48 (MQ=255)                                                                                                                                                                                                     gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcagg                                                                               >  1:610940‑M1/1‑176 (MQ=255)                                                                                                                                                                                                     gTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatgg                                                                                               >  1:621619‑M1/1‑176 (MQ=255)                                                                                                                                                                                                          gATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctga                              >  1:471959‑M1/1‑171 (MQ=255)                                                                                                                                                                                                           aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGt                                                                                                                                            <  1:172796/140‑1 (MQ=255)                                                                                                                                                                                                           aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaa                             >  1:446602‑M1/1‑170 (MQ=255)                                                                                                                                                                                                           aTCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgt                                                                                       <  1:349224‑M1/193‑24 (MQ=255)                                                                                                                                                                                                              atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGt                                                                                                                                                                                                       >  1:188712/1‑78 (MQ=255)                                                                                                                                                                                                              atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaagga                          >  1:722156‑M1/1‑167 (MQ=255)                                                                                                                                                                                                              atatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaagga                          >  1:690648‑M1/1‑167 (MQ=255)                                                                                                                                                                                                               tatTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGAt                                                                                                                                                                                                                              <  1:355605/54‑1 (MQ=255)                                                                                                                                                                                                                atTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgt                                                                                       >  1:181142‑M1/1‑165 (MQ=255)                                                                                                                                                                                                                atTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatat                                                                                                 <  1:336420‑M1/178‑14 (MQ=255)                                                                                                                                                                                                                 ttGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaata                       <  1:426788‑M1/251‑88 (MQ=255)                                                                                                                                                                                                                   gAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggt                                                                                              >  1:780432‑M1/1‑162 (MQ=255)                                                                                                                                                                                                                       aTGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgt                 <  1:842769‑M1/251‑94 (MQ=255)                                                                                                                                                                                                                        tGACGAAGCGGAGGAAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatat                      >  1:204574‑M1/1‑157 (MQ=255)                                                                                                                                                                                                                                  gaggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAAc                                                                                                                                                               >  1:853482/1‑98 (MQ=255)                                                                                                                                                                                                                                   aggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatac      <  1:702070‑M1/250‑105 (MQ=255)                                                                                                                                                                                                                                    ggaAATGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatat                      <  1:333155‑M1/233‑89 (MQ=255)                                                                                                                                                                                                                                      aaaTGGAAAGCTTGGAAACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAAGTCGGAAAGTGTAAAACTATTTCGGAAAAACAGGGTCAAACGGGCATTCTTGCTTCAGTTGCCATTaaaagccatatatggttatacgtaattcaggcttttaaggttgctgcagcgggacaatgattaatctattttcaacctgaaggaatatcatgttaaaaaaatacttac  <  1:187543‑M1/251‑109 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           CTTAATTATCGTGGCAAAGATCGCCCCACTAATGTGCTGTCTTTCCCTTTTGAATGCCCTGATGAAGTGGAGCTGTCGCTATTGGGCGATTTGGTGATTTGCCGTCAAGTGGTGGAGCGCGAAGCCCAAGAGCAAGACAAACCGCTCATGGCGTATTGGGCACACATGGTGGTGCATGGCGGTTTGCATTTACTCGGTTATGATCATATTGAAGATGACGAAGCGGAGGAAATGGAAAGCTTGGAGACGCAAATTATGATAGGGCTTGGCTTTGTCGATCCGTATCTGAGCGAGAAGTAAGAAAAATTCGGAATGTGTAAAACTATTTCGGAAAATCAGGGTCAAACGGGCATTCTTGCTTCAGTCGCCATTTATATTAAAGCCACATATAGTTACACGTAATTCAGACTTTTAAGGTTGCTGTAGCGAGGCAATGATTCATCTATTTTCAACCTGAAGGAATATCATGTTAAAAAAATA  >  1/885135‑885614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑