Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	869,732	T→C	F240F (TTT→TTC)	unknown →	Sodium‑dependent transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	869,732	0	T	C	100.0%	229.4 / NA	78	F240F (TTT→TTC)	unknown	Sodium‑dependent transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/43);  total (35/43)

CCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTTGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAGCGCCATTAGGGCAATTATTAGG  >  1/869488‑869977                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGtatt                                                                                                                                                                                                                                                 >  1:269313/1‑251 (MQ=255)     ttGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATgg                                                                                                                                                                                                                                             <  1:562121/251‑1 (MQ=255)     ttGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATgg                                                                                                                                                                                                                                             <  1:714005/251‑1 (MQ=255)        gCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATgg                                                                                                                                                                                                                                             <  1:276111/248‑1 (MQ=255)          tAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGAcc                                                                                                                                                                                                                                        <  1:36005/251‑1 (MQ=255)               aCTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGc                                                                                                                                                                                                                                   <  1:339600/251‑1 (MQ=255)               aCTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTAAGGTGACCTATg                                                                                                                                                                                                                                    >  1:765098/1‑250 (MQ=255)                   tatttattTTATCCCTCGGTGTTCAAAAAGGTGTTGCGCGCACTTCTGTTATTTTTTTTGCAATTTTAGTCATCATGTTTTTCTCTTTAATGGTTTATTCCTTATTTTTACCGGGGGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTcc                                                                                                                                                                                                                               <  1:847933/251‑1 (MQ=255)                   tATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAG‑AAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCt                                                                                                                                                                                                                              <  1:495096/251‑1 (MQ=255)                         ttttATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTg                                                                                                                                                                                                                         >  1:502285/1‑251 (MQ=255)                                     gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCg                                                                                                                                                                                                             >  1:865820/1‑251 (MQ=255)                                     gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCg                                                                                                                                                                                                             >  1:155287/1‑251 (MQ=255)                                     gtgtACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCg                                                                                                                                                                                                             >  1:734158/1‑251 (MQ=255)                                            aaaaGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTaa                                                                                                                                                                                                      >  1:391427/1‑251 (MQ=255)                                              aaGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAc                                                                                                                                                                                                     >  1:85995/1‑250 (MQ=255)                                              aaGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTCACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAcc                                                                                                                                                                                                    <  1:447954/251‑1 (MQ=255)                                                           cACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGAc                                                                                                                                                                                                                                         >  1:888698/1‑201 (MQ=255)                                                             cTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGAcc                                                                                                                                                                                                                                        >  1:644294/1‑200 (MQ=255)                                                             cTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTtggtg                                                                                                                                                                                     >  1:682560/1‑251 (MQ=255)                                                             cTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTtggtg                                                                                                                                                                                     >  1:342428/1‑251 (MQ=255)                                                                   tgATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGc                                                                                                                                                                               <  1:805785/251‑1 (MQ=255)                                                                   tgATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGc                                                                                                                                                                               <  1:897206/251‑1 (MQ=255)                                                                          tATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCa                                                                                                                                                                        <  1:561454/251‑1 (MQ=255)                                                                                 aTTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAAcagcag                                                                                                                                                                 >  1:431586/1‑251 (MQ=255)                                                                                 aTTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAAcagcag                                                                                                                                                                 >  1:289935/1‑251 (MQ=255)                                                                                          atcatGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCg                                                                                                                                                                                                   <  1:6641/208‑1 (MQ=255)                                                                                           tcatGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTTGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAgtg                                                                                                                                                       <  1:68666/251‑1 (MQ=255)                                                                                           tcatGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAgtg                                                                                                                                                       >  1:747729/1‑251 (MQ=255)                                                                                               gTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTgg                                                                                                                                                   >  1:855619/1‑251 (MQ=255)                                                                                                       cTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGta                                                                                                                                                                                                                                                   >  1:403680/1‑147 (MQ=255)                                                                                                       cTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCtt                                                                                                                                                                             <  1:415396/217‑1 (MQ=255)                                                                                                           aaTGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCg                                                                                                                                       <  1:867532/251‑1 (MQ=255)                                                                                                              ggTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTg                                                                                                                                                                                                                                           <  1:323792/148‑1 (MQ=255)                                                                                                              ggTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTAt                                                                                                                                    >  1:268306/1‑251 (MQ=255)                                                                                                               gTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATggctg                                                                                                                                                                                                                                          <  1:137366/148‑4 (MQ=255)                                                                                                               gTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTAtt                                                                                                                                   >  1:196502/1‑251 (MQ=255)                                                                                                                tttATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATtc                                                                                                                                  <  1:599736/251‑1 (MQ=255)                                                                                                                      ccTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGc                                                                                                                             >  1:156106/1‑250 (MQ=255)                                                                                                                         tATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTa                                                                                                                         >  1:357466/1‑251 (MQ=255)                                                                                                                            ttttACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGt                                                                                                                      <  1:462925/251‑1 (MQ=255)                                                                                                                            ttttACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGt                                                                                                                      <  1:329743/251‑1 (MQ=255)                                                                                                                                    ggCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTg                                                                                                               <  1:804043/250‑1 (MQ=255)                                                                                                                                      cgcAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCg                                                                                                            <  1:841252/251‑1 (MQ=255)                                                                                                                                          gAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTg                                                                                                         >  1:189282/1‑250 (MQ=255)                                                                                                                                                gggTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGc                                                                                                                                                                                                                                   >  1:52832/1‑122 (MQ=255)                                                                                                                                                gggTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTa                                                                                                                                                                                                                             <  1:268601/128‑1 (MQ=255)                                                                                                                                                gggTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACgg                                                                                                  >  1:191257/1‑251 (MQ=255)                                                                                                                                                         gCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAcc                                                                                                                                                                                                    <  1:768161/144‑1 (MQ=255)                                                                                                                                                         gCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGGATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGAtgt                                                                                         >  1:550393/1‑251 (MQ=255)                                                                                                                                                            cTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAg                                                                                      <  1:753167/251‑1 (MQ=255)                                                                                                                                                              gTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCg                                                                                    >  1:487963/1‑251 (MQ=255)                                                                                                                                                               ttCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCg                                                                                    <  1:141480/250‑1 (MQ=255)                                                                                                                                                                 ctcGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAg                                                                                 <  1:160255/251‑1 (MQ=255)                                                                                                                                                                        gATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGaca                                                                                               >  1:191698/1‑230 (MQ=255)                                                                                                                                                                        gATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCaa                                                                           <  1:843432/250‑1 (MQ=255)                                                                                                                                                                                  aaTTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCt                                                                                                                                                                                                                              <  1:486052/93‑1 (MQ=255)                                                                                                                                                                                  aaTTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGtata                                                                >  1:457180/1‑251 (MQ=255)                                                                                                                                                                                    ttAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAgg                                                              <  1:565518/251‑1 (MQ=255)                                                                                                                                                                                     tAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCg                                                                                    <  1:159245/228‑1 (MQ=255)                                                                                                                                                                                     tAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGt                                                             >  1:357597/1‑251 (MQ=255)                                                                                                                                                                                      aaaaGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCACCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGtc                                                            <  1:876590/251‑1 (MQ=255)                                                                                                                                                                                           aCCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGcc                                                                                                       >  1:685017/1‑203 (MQ=255)                                                                                                                                                                                                  cGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGcc                                                       >  1:827705/1‑244 (MQ=255)                                                                                                                                                                                                             ccGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAAcc                                     <  1:63792/251‑1 (MQ=255)                                                                                                                                                                                                             ccGCTTACGGACAAATATTCTTCTCCCTTTCCATGTGTTTCGGTATTATGGTGGCCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAAcc                                     <  1:103769/251‑1 (MQ=255)                                                                                                                                                                                                                      gACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGcc                                                                                                                                                 >  1:650703/1‑134 (MQ=255)                                                                                                                                                                                                                      gACAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTaa                             >  1:65269/1‑250 (MQ=255)                                                                                                                                                                                                                       aCAAATATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAAcc                           <  1:195641/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaTATTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGtat                                                                 <  1:715615/211‑1 (MQ=255)                                                                                                                                                                                                                            tatTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAgcg                      >  1:848955/1‑251 (MQ=255)                                                                                                                                                                                                                             atTCTTCTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAgcgc                     <  1:631572/251‑1 (MQ=255)                                                                                                                                                                                                                              ttcttcTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCACCGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAAcc                           >  1:426060/1‑244 (MQ=255)                                                                                                                                                                                                                              ttcttcTCCCTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGt                                                             <  1:884724/210‑1 (MQ=255)                                                                                                                                                                                                                                      ccTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAGCGCCATTAGGGc            <  1:34758/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccTTTCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAGCGCCATTAGGGc            <  1:146610/251‑1 (MQ=255)                                                                                                                                                                                                                                         ttCCATTTGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAAcc                                                                                                                                                                                                    <  1:74468/64‑1 (MQ=255)                                                                                                                                                                                                                                                tGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAGCGCCATTAGGGCAATTATTAgg  <  1:752556/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGTTTCGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAGCGCCATTAGGGCAATTATTAgg  <  1:90238/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CCGTTTGGCTAATTACTTTATTGATTTTATCCCTCGGTGTACAAAAAGGTGTTGCGCGCACTTCTGTGATTTTTATGCCAATTTTAGTCATCATGTTTGTCACTTTAATGGTTTATTCCTTATTTTTACCGGGCGCAGAAAAAGGGTTGGATGCCCTGTTCTCGCCGGATTGGGATAAATTAAAAGACCCGAGCGTCTGGATTGCCGCTTACGGACAAATATTCTTCTCCCTTTCCATTTGTTTTGGTATTATGGTGACCTATGCTTCCTATTTGAAAAAACAATCGGACTTAACCGGCGCCGGTTTGGTGGTAGGCTTTGCCAACAGCAGTTTTGAAGTGTTGGCCGGTATCGGCGTATTCTCCGCCTTAGGTTTCATTGCCGCTGCCAACGGACACGATGTGAGCGAAGTAGCAAAAGGCGGTATAGGTCTCGCCTTCTTTGCCTTCCCAACCATCATTAACCAAGCGCCATTAGGGCAATTATTAGG  >  1/869488‑869977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑