Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	864,374	C→T	G247G (GGC→GGT)	unknown →	L‑seryl‑tRNA(Sec) selenium transferase (EC 2.9.1.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	864,374	0	C	T	100.0%	221.6 / NA	77	G247G (GGC→GGT)	unknown	L‑seryl‑tRNA(Sec) selenium transferase (EC 2.9.1.1)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/33);  total (44/33)

GGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGCAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGTTGCGA  >  1/864128‑864610                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             gggCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAg                                                                                                                                                                                                                                          >  1:6321/1‑251 (MQ=255)   gCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGa                                                                                                                                                                                                                                         <  1:332909/250‑1 (MQ=255)    cAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGaa                                                                                                                                                                                                                                        <  1:889191/250‑1 (MQ=255)         aagTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGc                                                                                                                                                                                                                                  <  1:485950/251‑1 (MQ=255)              attattTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTc                                                                                                                                                                                                                              >  1:669549/1‑250 (MQ=255)                tattTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCgg                                                                                                                                                                                                                           <  1:552911/251‑1 (MQ=255)                     cccGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGtcat                                                                                                                                                                                                                       >  1:131957/1‑250 (MQ=255)                                cTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAg                                                                                                                                                                                                            <  1:103727/250‑1 (MQ=255)                                 tGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                                          <  1:257589/251‑1 (MQ=255)                                           cggcggCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGgcgcgc                                                                                                                                                                                                <  1:700974/251‑1 (MQ=255)                                                    gTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGAtt                                                                                                                                                                                       >  1:182351/1‑251 (MQ=255)                                                       ccGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTaa                                                                                                                                                                                    >  1:176610/1‑251 (MQ=255)                                                              cccGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAAtt                                                                                                                                                                             >  1:584113/1‑251 (MQ=255)                                                                     tcatGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTg                                                                                                                                                                      >  1:588162/1‑251 (MQ=255)                                                                          gcgcAGGCGGGCTGCAAGCCGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGa                                                                                                                                                                 >  1:143934/1‑251 (MQ=255)                                                                               ggCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTGAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAagag                                                                                                                                                             >  1:430713/1‑250 (MQ=255)                                                                                gCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAagag                                                                                                                                                             <  1:647276/249‑1 (MQ=255)                                                                                     cTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCg                                                                                                                                                      >  1:153403/1‑251 (MQ=255)                                                                                             tggtggAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGACTTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCCCCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGaa                                                                                                                                              >  1:433157/1‑251 (MQ=255)                                                                                              ggtggAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                                          <  1:794390/190‑1 (MQ=255)                                                                                              ggtggAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCg                                                                                                                                                      >  1:281261/1‑242 (MQ=255)                                                                                                  gAAGTGGTCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAAtt                                                                                                                                         >  1:795669/1‑251 (MQ=255)                                                                                                       gggCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAag                                                                                                                                                               <  1:660863/224‑1 (MQ=255)                                                                                                       gggCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTgcag                                                                                                                                     >  1:897096/1‑250 (MQ=255)                                                                                                       gggCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTgcag                                                                                                                                     >  1:872625/1‑250 (MQ=255)                                                                                                            cGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTgcagc                                                                                                                                    >  1:114033/1‑246 (MQ=255)                                                                                                                cAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGc                                                                                                                                                   >  1:313740/1‑227 (MQ=255)                                                                                                                  aCCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGgat                                                                                                                         >  1:798299/1‑251 (MQ=255)                                                                                                                          cATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGTTTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGgatttgattt                                                                                                                  >  1:283112/1‑250 (MQ=255)                                                                                                                          cATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGgatttgattt                                                                                                                  >  1:661700/1‑250 (MQ=255)                                                                                                                           aTTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                                          >  1:768930/1‑161 (MQ=255)                                                                                                                           aTTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGc                                                                                                                                                                                                          <  1:395231/161‑1 (MQ=255)                                                                                                                           aTTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGAt                                                                                                                                                                                        >  1:597091/1‑179 (MQ=255)                                                                                                                                        tCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGAt                                                                                                                                                                                        >  1:349668/1‑166 (MQ=255)                                                                                                                                        tCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACa                                                                                                   >  1:107979/1‑251 (MQ=255)                                                                                                                                              cGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTg                                                                                              >  1:654616/1‑250 (MQ=255)                                                                                                                                                cAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTg                                                                                              >  1:498778/1‑248 (MQ=255)                                                                                                                                                   ttAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGggc                                                                                        <  1:280725/251‑1 (MQ=255)                                                                                                                                                   ttAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGggc                                                                                        <  1:190528/251‑1 (MQ=255)                                                                                                                                                    tAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAg                                                                                                                                                                                                                                          >  1:483129/1‑104 (MQ=255)                                                                                                                                                             tACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAg                                                                              <  1:255535/251‑1 (MQ=255)                                                                                                                                                             tACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAg                                                                              <  1:588020/251‑1 (MQ=255)                                                                                                                                                                  ccTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCg                                                                            >  1:179141/1‑248 (MQ=255)                                                                                                                                                                        tAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCattatt                                                                   >  1:249921/1‑251 (MQ=255)                                                                                                                                                                         aaTGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATtg                                                                  <  1:605882/251‑1 (MQ=255)                                                                                                                                                                         aaTGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATtg                                                                  <  1:463948/251‑1 (MQ=255)                                                                                                                                                                         aaTGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATtg                                                                  <  1:430394/251‑1 (MQ=255)                                                                                                                                                                              aaGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGc                                                             <  1:12597/251‑1 (MQ=255)                                                                                                                                                                                     cagcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAg                                                      >  1:815239/1‑251 (MQ=255)                                                                                                                                                                                       gcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGggc                                                                                        >  1:197114/1‑215 (MQ=255)                                                                                                                                                                                       gcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCa                                                    >  1:96480/1‑251 (MQ=255)                                                                                                                                                                                       gcagTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAACACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCa                                                    >  1:454500/1‑251 (MQ=255)                                                                                                                                                                                           tAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCAc                                                   <  1:24257/248‑1 (MQ=255)                                                                                                                                                                                              cTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTc                                                                                                                                                                            >  1:180360/1‑124 (MQ=255)                                                                                                                                                                                                    aaTTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGc                                       <  1:232542/251‑1 (MQ=255)                                                                                                                                                                                                    aaTTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGAGTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGc                                       <  1:436911/251‑1 (MQ=255)                                                                                                                                                                                                            gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACaaa                                                                                                 <  1:175353/185‑1 (MQ=255)                                                                                                                                                                                                            gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCAc                                                   >  1:234941/1‑231 (MQ=255)                                                                                                                                                                                                            gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGc                                <  1:58779/250‑1 (MQ=255)                                                                                                                                                                                                            gTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGCCGACAAATTGCCGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGc                                >  1:308048/1‑250 (MQ=255)                                                                                                                                                                                                             ttttACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGcc                               <  1:484562/250‑1 (MQ=255)                                                                                                                                                                                                              tttACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCa                                                >  1:779604/1‑232 (MQ=255)                                                                                                                                                                                                                 aCCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCAccc                          <  1:189057/251‑1 (MQ=255)                                                                                                                                                                                                                 aCCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCAccc                          <  1:94879/251‑1 (MQ=255)                                                                                                                                                                                                                   ccACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCg                         >  1:584575/1‑250 (MQ=255)                                                                                                                                                                                                                        gCGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCaaa                   >  1:901360/1‑251 (MQ=255)                                                                                                                                                                                                                         cGTTGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCaaa                   <  1:787227/250‑1 (MQ=255)                                                                                                                                                                                                                           ttGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAAcg                 >  1:49913/1‑250 (MQ=255)                                                                                                                                                                                                                           ttGACGAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAAcg                 <  1:901835/250‑1 (MQ=255)                                                                                                                                                                                                                                gAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGtg             >  1:238294/1‑249 (MQ=255)                                                                                                                                                                                                                                gAGCAGGAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGtg             >  1:882839/1‑249 (MQ=255)                                                                                                                                                                                                                                      gAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGt       <  1:341640/249‑1 (MQ=255)                                                                                                                                                                                                                                      gAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGt       <  1:888124/249‑1 (MQ=255)                                                                                                                                                                                                                                      gAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGt       <  1:174185/249‑1 (MQ=255)                                                                                                                                                                                                                                      gAATTGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGTTg     >  1:281512/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttGGCGGATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGTTGCGa  <  1:652222/251‑1 (MQ=255)                                                                                                                                                                                                                                               gATTTGGGTAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCAc                                                   >  1:723763/1‑196 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GGGCAAAGAAGTGATTATTTCCCGTGGCGAGCTGATTGAAATCGGCGGCGCGTTCCGCATTCCCGACATCATGGCGCAGGCGGGCTGCAAGCTGGTGGAAGTGGGCACGACCAACCGCACCCATTTAAAAGATTATCGTAACGCAATTAACGAAAATACTGCCTTTTTAATGAAAGTTCACAGCAGTAACTATCAAATTTGCGGTTTTACCCACAGCGTTGACGAGCAGGAATTGGCGGATTTGGGCAAAGAATTCGGCATTCCGGTCATCACCGATTTAGGCAGCGGCGCGCTCATGGATTTAAAACAATTCGGCTTGCCGGAAGAGCCCACCGTGCAGGAAAAATTGCAGCACGGCGTGGATTTGATTTCCTTCTCCGGCGACAAATTGCTGGGCGGCCCGCAAGCGGGCATTATTGTGGGCAAAAAAGCACTCATTCAACAGCTGCAGGCCCACCCGCTCAAACGCGTGCTGCGTTGCGA  >  1/864128‑864610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑