Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	814,163	C→T	G1188D (GGC→GAC)	unknown ←	Inner membrane component of TAM transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	814,163	0	C	T	100.0%	213.6 / NA	71	G1188D (GGC→GAC)	unknown	Inner membrane component of TAM transport system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/38);  total (33/38)

GTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTGTCGCCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTAAAT  >  1/813928‑814394                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        gTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAgg                                                                                                                                                                                                                          >  1:766691/1‑251 (MQ=255)  tAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAgg                                                                                                                                                                                                                          <  1:865428/250‑1 (MQ=255)         gagTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCg                                                                                                                                                                                                                  >  1:887244/1‑251 (MQ=255)                  cGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAata                                                                                                                                                                                                         >  1:864217/1‑251 (MQ=255)                        aaCCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGAt                                                                                                                                                                                                   <  1:245809/251‑1 (MQ=255)                        aaCCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGAt                                                                                                                                                                                                   <  1:249455/251‑1 (MQ=255)                             tCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGc                                                                                                                                                                                              <  1:323667/251‑1 (MQ=255)                               aaaTAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGc                                                                                                                                                                                              >  1:257654/1‑249 (MQ=255)                                  tAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         <  1:468933/251‑1 (MQ=255)                                    aCCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATcc                                                                                                                                                                                       <  1:117808/251‑1 (MQ=255)                                         aCGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCgg                                                                                                                                                                                  <  1:368626/251‑1 (MQ=255)                                               tATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTc                                                                                                                                                                             >  1:459341/1‑250 (MQ=255)                                                       cTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         >  1:885871/1‑230 (MQ=255)                                                                cgGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATgg                                                                                                                                                           <  1:600865/251‑1 (MQ=255)                                                                  gtgtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAatat                                                                                                                                                                                                        <  1:611508/204‑1 (MQ=255)                                                                  gtgtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATcc                                                                                                                                                                                       >  1:234796/1‑221 (MQ=255)                                                                  gtgtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGtt                                                                                                                                                         >  1:195261/1‑251 (MQ=255)                                                                    gtAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTa                                                                                                                                                        >  1:143414/1‑250 (MQ=255)                                                                       aTGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACt                                                                                                                                                    <  1:723042/251‑1 (MQ=255)                                                                                    ccACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGAt                                                                                                                                                                                                   >  1:116215/1‑191 (MQ=255)                                                                                    ccACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCg                                                                                                                                         >  1:203288/1‑249 (MQ=255)                                                                                       ctactTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCtata                                                                                                                                    >  1:339803/1‑251 (MQ=255)                                                                                        tactTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:372141/251‑1 (MQ=255)                                                                                        tactTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGAATCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:894009/251‑1 (MQ=255)                                                                                         actTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATg                                                                                                                                                            <  1:466457/225‑1 (MQ=255)                                                                                         actTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAcc                                                                                                                                  <  1:884540/251‑1 (MQ=255)                                                                                          ctTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTa                                                                                                                                                                                                              <  1:273812/174‑1 (MQ=255)                                                                                          ctTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAcc                                                                                                                                  >  1:367336/1‑250 (MQ=255)                                                                                          ctTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCa                                                                                                                                 <  1:798283/251‑1 (MQ=255)                                                                                           tttAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                <  1:223013/251‑1 (MQ=255)                                                                                           tttAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                <  1:81946/251‑1 (MQ=255)                                                                                           tttAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                <  1:452445/251‑1 (MQ=255)                                                                                                     tCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTcac                                                                                                                      <  1:222703/251‑1 (MQ=255)                                                                                                        cccACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGTGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCacac                                                                                                                    <  1:461864/250‑1 (MQ=255)                                                                                                        cccACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCacac                                                                                                                    <  1:206660/250‑1 (MQ=255)                                                                                                            ccccGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   >  1:386381/1‑231 (MQ=255)                                                                                                            ccccGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCa                                                                                                               >  1:467993/1‑251 (MQ=255)                                                                                                            ccccGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCa                                                                                                               >  1:644632/1‑251 (MQ=255)                                                                                                                 gcggTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         >  1:569850/1‑172 (MQ=255)                                                                                                                           ggTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:609629/216‑1 (MQ=255)                                                                                                                           ggTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGt                                                                                                  >  1:557757/1‑249 (MQ=255)                                                                                                                            gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCAt                                                                                                                                >  1:383033/1‑218 (MQ=255)                                                                                                                            gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTaa                                                                                                           >  1:40166/1‑239 (MQ=255)                                                                                                                            gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGt                                                                                                  <  1:837418/248‑1 (MQ=255)                                                                                                                            gTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCt                                                                                                <  1:330104/250‑1 (MQ=255)                                                                                                                                    cTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTac                                                                                                                                                       >  1:517768/1‑187 (MQ=255)                                                                                                                                          tACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGa                                                                                 >  1:31120/1‑251 (MQ=255)                                                                                                                                          tACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGa                                                                                 <  1:253074/251‑1 (MQ=255)                                                                                                                                             cAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAg                                                                                                                                                                                                     >  1:287701/1‑132 (MQ=255)                                                                                                                                                  gCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGaa                                                                          <  1:497042/250‑1 (MQ=255)                                                                                                                                                    ttttCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTg                                                                       >  1:126043/1‑251 (MQ=255)                                                                                                                                                               ccccGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTc                                                            <  1:77677/251‑1 (MQ=255)                                                                                                                                                                   gACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAcc                                                                                                                                  >  1:362487/1‑177 (MQ=255)                                                                                                                                                                        caTTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGt                                                   >  1:241953/1‑251 (MQ=255)                                                                                                                                                                        caTTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTG‑TTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGtt                                                  >  1:117127/1‑251 (MQ=255)                                                                                                                                                                                cTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATAc                                                                                                                                   <  1:597606/163‑1 (MQ=255)                                                                                                                                                                                cTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTc                                                            <  1:272419/234‑1 (MQ=255)                                                                                                                                                                                     aGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGAt                                                                                                                                                                                         <  1:706996/104‑1 (MQ=255)                                                                                                                                                                                          gCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACa                                 >  1:405626/1‑251 (MQ=255)                                                                                                                                                                                           ccccATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTacac                                                                                                                                                     >  1:461732/1‑134 (MQ=255)                                                                                                                                                                                           ccccATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACaa                                >  1:305536/1‑251 (MQ=255)                                                                                                                                                                                              cATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAAc                             <  1:13503/251‑1 (MQ=255)                                                                                                                                                                                               aTGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAgg                                                                                                                                                                                                                          >  1:250963/1‑61 (MQ=255)                                                                                                                                                                                                       cAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGc                     <  1:818191/250‑1 (MQ=255)                                                                                                                                                                                                        aaaGCGGCGCCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGccc                   <  1:297078/251‑1 (MQ=255)                                                                                                                                                                                                               cgcCCACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACg             <  1:608647/250‑1 (MQ=255)                                                                                                                                                                                                                 cccACAGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGaat          <  1:868274/251‑1 (MQ=255)                                                                                                                                                                                                                   cacaGAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTg                                                 <  1:103465/210‑1 (MQ=255)                                                                                                                                                                                                                       gAACCGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTaa    >  1:677507/1‑251 (MQ=255)                                                                                                                                                                                                                          ccGCTGGAAGCGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTAAAt  >  1:492491/1‑250 (MQ=255)                                                                                                                                                                                                                                    cGGTATCGTCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAAc                             <  1:379191/213‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                        GTAACGCAGAGTCACTTCGGCTAAACCGTCAAATAACCCAACGCCGTATTTTACCTGTAAGCGCGGTGTAATGGTGCCGCTGACCACTACTTTAGATCTGTCGCCCACCCCGGCGGTACTCAGGTTCAGATCTTTGATACCAAAGGCTTTTCCGATTCCCCCGACCACATTACCGCTTTTAGCGAGCCCCATGCCGAGCAAAGCGGCGCCCACAGAACCGCTGGAAGCGGTGTCGCCGCTGTTTTCTAAGGAGCGGCCGGTTAAAATATAAGATAGTGCCTGATCCTGCGGCATTCCCGGTTCGGAGAAAATGGTTACACTGGGTGCGGTCGCTATACCATTCACTTTCACACCGGCAGTAACGCCTGCGTCTTCCATGGCTTCCGGATTACGAATTGCTTCAATATTCAGCATCGGTTGCGACGGTTGACCGACAAAACTGATGAGCCCTTTACGAATAATTAAAT  >  1/813928‑814394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑