Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	790,914	G→A	R110R (CGC→CGT)	unknown ←	Inner membrane protein YihY, formerly thought to be RNase BN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	790,914	0	G	A	100.0%	242.4 / NA	82	R110R (CGC→CGT)	unknown	Inner membrane protein YihY, formerly thought to be RNase BN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/42);  total (40/42)

TTTTTTATTCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGGTGTCATGCCAAATGCCGTTCAGGGTGCGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGCACAATGGCGAGCATGGTGCTGTA  >  1/790668‑791159                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ttttttATTCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGt                                                                                                                                                                                                                                                   <  1:476563/251‑1 (MQ=255) ttttttATTCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGt                                                                                                                                                                                                                                                   <  1:856157/251‑1 (MQ=255)  tttttATTCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGt                                                                                                                                                                                                                                                   >  1:286836/1‑250 (MQ=255)        ttCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGa                                                                                                                                                                                                                                            <  1:734812/251‑1 (MQ=255)        ttCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGa                                                                                                                                                                                                                                            <  1:817162/251‑1 (MQ=255)               ccaccaTATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATc                                                                                                                                                                                                                                     <  1:893494/251‑1 (MQ=255)               ccaccaTATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATc                                                                                                                                                                                                                                     <  1:28968/251‑1 (MQ=255)                   caTATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACa                                                                                                                                                                                                                                 >  1:760357/1‑251 (MQ=255)                    atatAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAATCTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACAt                                                                                                                                                                                                                                >  1:94238/1‑251 (MQ=255)                      ataAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCa                                                                                                                                                                                                                              >  1:278678/1‑251 (MQ=255)                        aaaTCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAAt                                                                                                                                                                                                                            >  1:369567/1‑251 (MQ=255)                          aTCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGc                                                                                                                                                                                                                          <  1:270935/251‑1 (MQ=255)                           acACGGTGAAAATAAACCAGGGGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGcc                                                                                                                                                                                                                         <  1:306683/250‑1 (MQ=255)                                 tGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCcaccac                                                                                                                                                                                                                    <  1:95153/250‑1 (MQ=255)                                    aaaTAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAAtt                                                                                                                                                                                                                >  1:199647/1‑251 (MQ=255)                                       tAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTaaa                                                                                                                                                                                                             >  1:635587/1‑251 (MQ=255)                                        aaaCCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAAc                                                                                                                                                                                                            <  1:873230/251‑1 (MQ=255)                                              ggTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACtgatga                                                                                                                                                                                                      >  1:339299/1‑251 (MQ=255)                                                   aaagaaaCGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 <  1:532986/251‑1 (MQ=255)                                                   aaagaaaCGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 <  1:45652/251‑1 (MQ=255)                                                   aaagaaaCGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 <  1:799429/251‑1 (MQ=255)                                                   aaagaaaCGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAcc                                                                                                                                                                                                  <  1:81469/250‑1 (MQ=255)                                                   aaagaaaCGGCACAAAACAGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACAGAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAGAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTGCAGGGTACGGTCGATGGAAGTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 <  1:617368/251‑1 (MQ=255)                                                       aaaCGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCAc                                                                                                                                                                                               <  1:242058/249‑1 (MQ=255)                                                            gCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGt                                                                                                                                                                                                                                                   <  1:722125/192‑1 (MQ=255)                                                            gCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 >  1:169817/1‑242 (MQ=255)                                                               caAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACCGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATc                                                                                                                                                                                     <  1:451980/251‑1 (MQ=255)                                                                   aCTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 <  1:60359/235‑1 (MQ=255)                                                                            tttCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAattat                                                                                                                                                                        >  1:233773/1‑251 (MQ=255)                                                                                   ccGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACaaa                                                                                                                                                                 <  1:68642/251‑1 (MQ=255)                                                                                         gaagaagCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAAc                                                                                                                                                                                                            >  1:234484/1‑202 (MQ=255)                                                                                                cTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAatatat                                                                                                                                                    <  1:291585/251‑1 (MQ=255)                                                                                                cTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAatatat                                                                                                                                                    >  1:369543/1‑251 (MQ=255)                                                                                                  gCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATc                                                                                                                                                                                                                                     >  1:845644/1‑168 (MQ=255)                                                                                                  gCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATc                                                                                                                                                                                     >  1:411970/1‑216 (MQ=255)                                                                                                  gCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAatatat                                                                                                                                                    >  1:726626/1‑249 (MQ=255)                                                                                                     gAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCAc                                                                                                                                                                                          <  1:573318/208‑1 (MQ=255)                                                                                                      aaTACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGccc                                                                                                                                               <  1:4970/250‑1 (MQ=255)                                                                                                      aaTACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGcc                                                                                                                                                <  1:780690/249‑1 (MQ=255)                                                                                                       aTACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCcca                                                                                                                                             >  1:481537/1‑251 (MQ=255)                                                                                                          cTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCccacca                                                                                                                                          <  1:847788/251‑1 (MQ=255)                                                                                                           tCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCcaccac                                                                                                                                         <  1:183514/251‑1 (MQ=255)                                                                                                                      tttCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAatat                                                                                                                                                      <  1:709199/227‑1 (MQ=255)                                                                                                                           caTAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCAc                                                                                                                                    <  1:760059/240‑1 (MQ=255)                                                                                                                           caTAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCggaggga                                                                                                                         >  1:364496/1‑251 (MQ=255)                                                                                                                           caTAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCggaggga                                                                                                                         >  1:391248/1‑251 (MQ=255)                                                                                                                             tAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCggagggag                                                                                                                        >  1:73816/1‑250 (MQ=255)                                                                                                                              aaGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAAGCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCg                                                                                                                      <  1:435421/251‑1 (MQ=255)                                                                                                                                            aTACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAatat                                                                                                                                                      >  1:508703/1‑205 (MQ=255)                                                                                                                                            aTACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAAt                                                                                                        >  1:156818/1‑251 (MQ=255)                                                                                                                                                    gCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAAc                                                                                                                                                                                                            >  1:518917/1‑143 (MQ=255)                                                                                                                                                       tactaACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAAc                                                                                                                                                                                                            <  1:142142/140‑1 (MQ=255)                                                                                                                                                       tactaACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCa                                                                                             >  1:840513/1‑251 (MQ=255)                                                                                                                                                                    ccTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAAc                                                                                <  1:832615/251‑1 (MQ=255)                                                                                                                                                                       aaCGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGt                                                                                                                                                                                                                                                   >  1:753067/1‑85 (MQ=255)                                                                                                                                                                       aaCGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTc                                                                             <  1:733001/251‑1 (MQ=255)                                                                                                                                                                         cGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAAc                                                                                                                                                                                                            <  1:356952/122‑1 (MQ=255)                                                                                                                                                                          gTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGtt                                                                          >  1:142042/1‑251 (MQ=255)                                                                                                                                                                              aaaTCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGACGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTaaaa                                                                      <  1:425441/251‑1 (MQ=255)                                                                                                                                                                                aTCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAAc                                                                    <  1:399470/251‑1 (MQ=255)                                                                                                                                                                                                      agGTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATgg                                                <  1:249751/249‑1 (MQ=255)                                                                                                                                                                                                       ggTAAAAATCGGGCGGGTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAActc                                                                                                  <  1:774216/198‑1 (MQ=255)                                                                                                                                                                                                                      ggTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATAccc                                                                                                                                                                                                 <  1:119586/88‑1 (MQ=255)                                                                                                                                                                                                                      ggTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTaaaa                                                                      <  1:593149/211‑1 (MQ=255)                                                                                                                                                                                                                      ggTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATc                               <  1:880340/250‑1 (MQ=255)                                                                                                                                                                                                                      ggTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAATAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATaa                                 >  1:428943/1‑248 (MQ=255)                                                                                                                                                                                                                       gTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCAc                                                                                                                                                                                               >  1:407006/1‑89 (MQ=255)                                                                                                                                                                                                                       gTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCaa                                                                                            >  1:100550/1‑188 (MQ=255)                                                                                                                                                                                                                       gTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATc                               >  1:248849/1‑249 (MQ=255)                                                                                                                                                                                                                       gTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAg                             >  1:338221/1‑251 (MQ=255)                                                                                                                                                                                                                       gTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAg                             >  1:243797/1‑251 (MQ=255)                                                                                                                                                                                                                       gTACTGTTGTCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAg                             >  1:699271/1‑251 (MQ=255)                                                                                                                                                                                                                            gttgtCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATc                                                                                                                                                                                     >  1:850165/3‑94 (MQ=255)                                                                                                                                                                                                                            gttgtCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGca                        >  1:526449/3‑251 (MQ=255)                                                                                                                                                                                                                               gtCATGCCAAATGCCGTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATACACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGCACaa                     >  1:451848/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTTCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACcggcgg                                                                                                                              >  1:326529/1‑131 (MQ=255)                                                                                                                                                                                                                                                tCAGGGTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATc                               >  1:704535/1‑224 (MQ=255)                                                                                                                                                                                                                                                   gggTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAAc                                                                                                                                                                                                            <  1:737097/48‑1 (MQ=255)                                                                                                                                                                                                                                                   gggTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATc                               <  1:565597/221‑1 (MQ=255)                                                                                                                                                                                                                                                   gggTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGCACAATGGCGAGCATGGTGCTGTa  >  1:742310/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gggTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGCACAATGGCGAGCATGGTGCTGTa  >  1:570504/1‑250 (MQ=255)                                                                                                                                                                                                                                                   gggTACGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGCACAATGGCGAGCATGGTGCTGTa  >  1:93072/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TTTTTTATTCGGCACCACCATATAAATCACGGTGAAAATAAACCAGGTGGAAAGAAACGGCACAAAACTGAGTAATTTCAAACCGAAAGAAGAAGCTGCAGAATACTCAAACATGGTTTTCACATAAGAACTGGCGGCAATACTGGTGCCTACTAACAGCGGACCTAACGTCAAAATCAGCCAATAAATCGCAAAAGAGGTAAAAATCGGGCGGGTACTGGTGTCATGCCAAATGCCGTTCAGGGTGCGGTCGATGGAATTAATCAACATCAATGCCACCACAATTAAACTGATGATACCCACCGCACTCATCTGTTTGGAATTATTCACAAATTCATCAATATATTGCCCCACCACGTCACCGGCGGAGGGAGCGAAATTAGTAAAAATAAACTCTTTCAACGCACCGGTAACTTCGTTAAAAACCGGAAAAGCGGAGAAGATGGAAAACACCACCATAATCAGCGGCACAATGGCGAGCATGGTGCTGTA  >  1/790668‑791159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑