Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	788,833	A→G	R328R (CGT→CGC)	unknown ←	Sodium/glutamate symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	788,833	0	A	G	100.0%	233.4 / NA	78	R328R (CGT→CGC)	unknown	Sodium/glutamate symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/37);  total (41/37)

GTTTCTATAAATTCAATTAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATACGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGACACCATACGAATGTTGG  >  1/788592‑789078                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ttttCTATAAATTCAATTAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTc                                                                                                                                                                                                                                              <  1:9666/250‑1 (MQ=255)          aaTTCAATTAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAAc                                                                                                                                                                                                                                      >  1:532700/1‑250 (MQ=255)              cAATTAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCaa                                                                                                                                                                                                                                 >  1:313454/1‑251 (MQ=255)              cAATTAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCaa                                                                                                                                                                                                                                 >  1:704407/1‑251 (MQ=255)                  tAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGAc                                                                                                                                                                                                                             <  1:702140/251‑1 (MQ=255)                    gTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGa                                                                                                                                                                                                                              >  1:458619/1‑248 (MQ=255)                         aTGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATa                                                                                                                                                                                                                      >  1:81941/1‑251 (MQ=255)                           gCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTaa                                                                                                                                                                                                                                                 <  1:899501/222‑1 (MQ=255)                               aTACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGaac                                                                                                                                                                                                                 <  1:93122/250‑1 (MQ=255)                               aTACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGaac                                                                                                                                                                                                                 <  1:444241/250‑1 (MQ=255)                                  ccACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGaacaac                                                                                                                                                                                                              >  1:194177/1‑250 (MQ=255)                                  ccACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGa                                                                                                                                                                                                                   >  1:159645/1‑245 (MQ=255)                                       aaTAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAAt                                                                                                                                                                                                        <  1:72401/251‑1 (MQ=255)                                                                    tACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAAcc                                                                                                                                                                                                             <  1:204943/217‑1 (MQ=255)                                                                          gTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAAc                                                                                                                                                                                                                                      <  1:844238/186‑1 (MQ=255)                                                                          gTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAAt                                                                                                                                                                                                        <  1:83020/216‑1 (MQ=255)                                                                          gTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAAcc                                                                                                                                                                        >  1:176404/1‑248 (MQ=255)                                                                                     aCGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCAt                                                                                                                                                          <  1:52758/251‑1 (MQ=255)                                                                                               cATCGGTACATTTAAGAAAGTTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAgg                                                                                                                                                <  1:736489/251‑1 (MQ=255)                                                                                               cATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCGCATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAgg                                                                                                                                                <  1:353690/251‑1 (MQ=255)                                                                                               cATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAgg                                                                                                                                                <  1:641764/251‑1 (MQ=255)                                                                                               cATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAgg                                                                                                                                                <  1:678223/251‑1 (MQ=255)                                                                                                       cAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAAt                                                                                                                                        >  1:409477/1‑251 (MQ=255)                                                                                                         aTTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGaac                                                                                                                                                                                                                 >  1:325081/1‑176 (MQ=255)                                                                                                         aTTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGc                                                                                                                                      <  1:891945/251‑1 (MQ=255)                                                                                                         aTTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGc                                                                                                                                      <  1:219123/251‑1 (MQ=255)                                                                                                                 aGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGc                                                                                                                                      >  1:6/1‑243 (MQ=255)                                                                                                                  gCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATaa                                                                                                                                                                                                                                        <  1:762390/144‑1 (MQ=255)                                                                                                                  gCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAgg                                                                                                                                                <  1:206581/232‑1 (MQ=255)                                                                                                                  gCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTaa                                                                                                                             >  1:466178/1‑251 (MQ=255)                                                                                                                     ttGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTGATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGc                                                                                                                                      <  1:831356/239‑1 (MQ=255)                                                                                                                     ttGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGCAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGaa                                                                                                                          >  1:417943/1‑251 (MQ=255)                                                                                                                                 ccAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGAcc                                                                                                              <  1:369726/251‑1 (MQ=255)                                                                                                                                 ccAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGAcc                                                                                                              <  1:137135/251‑1 (MQ=255)                                                                                                                                         gCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTa                                                                                                                              >  1:479460/1‑227 (MQ=255)                                                                                                                                                 ccGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACa                                                                                              >  1:519089/1‑251 (MQ=255)                                                                                                                                                   gCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGAc                                                                                                                                                                                                                             <  1:290056/122‑1 (MQ=255)                                                                                                                                                   gCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGAc                                                                                                                   <  1:688004/228‑1 (MQ=255)                                                                                                                                                     cTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTa                                                                                          >  1:416684/1‑251 (MQ=255)                                                                                                                                                          tGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCa                                                                                     >  1:783835/1‑251 (MQ=255)                                                                                                                                                                  aCTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGAc                                                                                                                   <  1:754901/213‑1 (MQ=255)                                                                                                                                                                            gtgtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGAc                                                                                                                   >  1:441371/1‑203 (MQ=255)                                                                                                                                                                            gtgtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAAt                                                                   >  1:825190/1‑251 (MQ=255)                                                                                                                                                                            gtgtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAAt                                                                   >  1:697702/1‑251 (MQ=255)                                                                                                                                                                            gtgtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAAt                                                                   >  1:605216/1‑251 (MQ=255)                                                                                                                                                                            gtgtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAAt                                                                   >  1:142077/1‑251 (MQ=255)                                                                                                                                                                            gtgtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAAt                                                                   >  1:888809/1‑251 (MQ=255)                                                                                                                                                                              gtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGAc                                                                                                                   >  1:262151/1‑201 (MQ=255)                                                                                                                                                                              gtTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAgtagcgaacatgcaggcggt                                                                 >  1:604573/1‑232 (MQ=255)                                                                                                                                                                                 gCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACt                                                                                                                                                                                                                            <  1:499464/93‑1 (MQ=255)                                                                                                                                                                                        ggCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTAccc                                                                                                    <  1:199324/206‑1 (MQ=255)                                                                                                                                                                                             aaaCCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCaa                                                  >  1:622261/1‑251 (MQ=255)                                                                                                                                                                                               aCCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGc                                                                                                                                      >  1:24556/1‑165 (MQ=255)                                                                                                                                                                                                ccACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGa                                               <  1:581593/251‑1 (MQ=255)                                                                                                                                                                                                       gACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTaa                                                                                                                                         <  1:332654/154‑1 (MQ=255)                                                                                                                                                                                                        aCCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAATAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGa                                        <  1:440519/250‑1 (MQ=255)                                                                                                                                                                                                            gCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGCCAAACCCCCTTTACCAAAAACATCTACGCCAGAATCAGCAACATTAAATTTGAAAATATGGTTAAAAAAATTACGaagaat                                   >  1:711830/1‑247 (MQ=255)                                                                                                                                                                                                            gCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGAt                                   >  1:12655/1‑251 (MQ=255)                                                                                                                                                                                                            gCACTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGAt                                   >  1:762852/1‑251 (MQ=255)                                                                                                                                                                                                              aCTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGa                                                                                                                                                                                                                   >  1:852037/1‑73 (MQ=255)                                                                                                                                                                                                               cTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATaa                                                                                                                                                                                                                                        <  1:647894/51‑1 (MQ=255)                                                                                                                                                                                                               cTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAAt                                                                                                                                   <  1:87571/152‑1 (MQ=255)                                                                                                                                                                                                               cTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGAc                                                                                                                   <  1:70176/168‑1 (MQ=255)                                                                                                                                                                                                               cTTAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTa                                 >  1:227090/1‑250 (MQ=255)                                                                                                                                                                                                                 tAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGAc                                                                                                                                                                                                                             <  1:360920/60‑1 (MQ=255)                                                                                                                                                                                                                 tAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAAccc                                                                                                                                                                       <  1:844405/114‑1 (MQ=255)                                                                                                                                                                                                                 tAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGc                                                                                                                                      >  1:645946/1‑147 (MQ=255)                                                                                                                                                                                                                 tAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTAcc                               >  1:240419/1‑250 (MQ=255)                                                                                                                                                                                                                 tAATACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTAcc                               >  1:512713/1‑250 (MQ=255)                                                                                                                                                                                                                   aTACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAg                                                                                                                                                        >  1:799959/1‑127 (MQ=255)                                                                                                                                                                                                                   aTACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGt                                                                                                         >  1:44292/1‑174 (MQ=255)                                                                                                                                                                                                                   aTACTACTGCGTCGTAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCg                            >  1:186835/1‑251 (MQ=255)                                                                                                                                                                                                                             gtcgtAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGa                                                                                                                                                                                                                   >  1:294939/1‑58 (MQ=255)                                                                                                                                                                                                                              tcgtAATCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGac                  <  1:777339/250‑1 (MQ=255)                                                                                                                                                                                                                               cgtAATCTTTACCCATTATGCGATAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGacac                <  1:3035/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCTTTACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGACACCATa            >  1:169476/1‑250 (MQ=255)                                                                                                                                                                                                                                       ttACCCATTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTTACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGACACCATACGaa        <  1:546397/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTTATGCGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGACACCATACGAATGTTgg  >  1:521314/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      GTTTCTATAAATTCAATTAGTGCAATGCGGATACCACAAATAAAAAGACTTTGAGCACGGAAGCATTTACCAAGTCAATGAAGAACGCGCCAACCATCGGTACAATTAAGAAAGCTTTGTGTGACGGTCCAAAACGGCTTGTCACCGCCTGCATGTTCGCTACTGCCGTCGGTGTTGCACCAAGGCCGAAACCACAGTGACCGGCACTTAATACTACTGCGTCGTAATCTTTACCCATTATACGGTAAGTCACATAAACCGCAAAGACTGCCATAAGAACAACCTGAATTGCCAAAATGACCATGACCGGTAACGCCAACCCTGCCAATTGCCATAGTTTTAAGGACATTAATGCAATAGCTAAGAATAAGGACAGACCCACGTTACCCAAAACATCTACGGCAGAATCAGCAACATTAAATTTGAAAATATGGGTCAAAGAATTACGAATGATTACCCCGGTAAACAGACACCATACGAATGTTGG  >  1/788592‑789078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑