Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	784,418	A→G	G35G (GGT→GGC)	unknown ←	Inner membrane protein YejM, alkaline phosphatase superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	784,418	0	A	G	100.0%	187.1 / NA	67	G35G (GGT→GGC)	unknown	Inner membrane protein YejM, alkaline phosphatase superfamily
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/32);  total (35/32)

CGAACAAAAACTTCCGTATCCACTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCACCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGATTAAGGATA  >  1/784191‑784657                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                cGAACAAAAACTTCCGTATCCACTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGaa                                                                                                                                                                                                                           <  1:45900/250‑1 (MQ=255)      aaaaaCTTCCGTATCCACTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaa                                                                                                                                                                                                                     <  1:711958/251‑1 (MQ=255)                gTATCCACTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCaaa                                                                                                                                                                                                           >  1:55555/1‑251 (MQ=255)                gTATCCACTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCaaa                                                                                                                                                                                                           >  1:538121/1‑251 (MQ=255)                       cTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTg                                                                                                                                                                                                     >  1:676793/1‑250 (MQ=255)                       cTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTg                                                                                                                                                                                                     >  1:135840/1‑250 (MQ=255)                       cTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTGGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaaaa                                                                                                                                                                                                                   <  1:445722/236‑1 (MQ=255)                         aataataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaaaa                                                                                                                                                                                                                   <  1:179268/234‑1 (MQ=255)                         aataataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGcc                                                                                                                                                                                                   >  1:646148/1‑250 (MQ=255)                          ataataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaaaa                                                                                                                                                                                                                   <  1:772034/233‑1 (MQ=255)                          ataataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaa                                                                                                                                                                                                                     <  1:857815/231‑1 (MQ=255)                          ataataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGccc                                                                                                                                                                                                  >  1:766793/1‑250 (MQ=255)                           taataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaa                                                                                                                                                                                                                     <  1:704238/230‑1 (MQ=255)                           taataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGcccc                                                                                                                                                                                                 >  1:471987/1‑250 (MQ=255)                           taataaGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGccc                                                                                                                                                                                                  <  1:702526/249‑1 (MQ=255)                                                     gAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTttctt                                                                                                                                                                        >  1:859655/1‑249 (MQ=255)                                                      aaTAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTtcttct                                                                                                                                                                      <  1:682950/250‑1 (MQ=255)                                                      aaTAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTtcttct                                                                                                                                                                      >  1:722181/1‑250 (MQ=255)                                                      aaTAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTtcttct                                                                                                                                                                      <  1:450973/250‑1 (MQ=255)                                                      aaTAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTtcttct                                                                                                                                                                      <  1:869100/250‑1 (MQ=255)                                                      aaTAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTtcttct                                                                                                                                                                      <  1:443210/250‑1 (MQ=255)                                                           cGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaa                                                                                                                                                                                                                     <  1:200803/198‑1 (MQ=255)                                                           cGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGatat                                                                                                                                                                >  1:832913/1‑251 (MQ=255)                                                                          aaGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAAt                                                                                                                                                 <  1:884547/251‑1 (MQ=255)                                                                          aaGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAAt                                                                                                                                                 <  1:35296/251‑1 (MQ=255)                                                                                   ggtTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACGATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATa                                                                                                                                        >  1:86421/1‑251 (MQ=255)                                                                                      ttttGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATg                                                                                                                                     <  1:44557/251‑1 (MQ=255)                                                                                            cGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAg                                                                                                                                                                                                       >  1:592800/1‑179 (MQ=255)                                                                                            cGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTg                                                                                                                               >  1:861725/1‑251 (MQ=255)                                                                                                      gAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGatat                                                                                                                                                                >  1:494980/1‑208 (MQ=255)                                                                                                            gAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAaga                                                                                                               >  1:49790/1‑251 (MQ=255)                                                                                                            aaaaaTCACCAGCAAATAAAAGGCGAAACCAATAAAACGGAAATGCCCTAAAAAACGGATG‑AAAAAATCCACTTTACCAAATAAGGTGTCGGGCAAGTCAATAATAAAAGCATAACGGGCGCGAATAAAAATCGCCCACAGAATATT‑AAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAagag                                                                                                              <  1:471785/250‑1 (MQ=255)                                                                                                             aaaaTCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAagag                                                                                                              <  1:333415/251‑1 (MQ=255)                                                                                                             aaaaTCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAagag                                                                                                              <  1:143833/251‑1 (MQ=255)                                                                                                                aTCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGt                                                                                                           >  1:471778/1‑251 (MQ=255)                                                                                                                         agATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTATCGatat                                                                                                                                                                >  1:235843/1‑189 (MQ=255)                                                                                                                          gATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGt                                                                                                                                                             >  1:431730/1‑191 (MQ=255)                                                                                                                          gATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAagagag                                                                                                            <  1:825046/240‑1 (MQ=255)                                                                                                                                 ggCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATGAAAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCaa                                                                                                    >  1:880042/1‑242 (MQ=255)                                                                                                                                 ggCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAagag                                                                                                              >  1:116625/1‑231 (MQ=255)                                                                                                                                         cAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGgag                                                                                          >  1:105300/1‑243 (MQ=255)                                                                                                                                               aaCTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTAtt                                                                                                                                <  1:365973/199‑1 (MQ=255)                                                                                                                                                   gAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGc                                                                               >  1:894544/1‑244 (MQ=255)                                                                                                                                                        gCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCAc                                                                         >  1:473864/1‑245 (MQ=255)                                                                                                                                                        gCCCTAATAAACTGATG‑AAAAAAGACACTTTACCGAAGAAGGTGTCGGGCCAGTCGATAAGAAAAGCATAACGGGCGACGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTGTGCGAGGTTTCTTCGCGAGATTGTTTGAGGGGAAATTTCCACAGAAGGCTATTGGTTCGTCGTATTAagag                                                                                                              <  1:255542/208‑1 (MQ=255)                                                                                                                                                           cTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGc                                                                                                                                    <  1:118491/183‑1 (MQ=255)                                                                                                                                                             aataaACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTg                                                                >  1:327377/1‑249 (MQ=255)                                                                                                                                                                          aaaaaaTACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCAc                                                                         >  1:672664/1‑228 (MQ=255)                                                                                                                                                                          aaaaaaTACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGacac                                                  <  1:489030/251‑1 (MQ=255)                                                                                                                                                                            aaaaTACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGaca                                                   >  1:353245/1‑248 (MQ=255)                                                                                                                                                                                 acacTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACAT‑ATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAg                                           >  1:225657/1‑250 (MQ=255)                                                                                                                                                                                      ttACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGtt                                                                                                                            >  1:93762/1‑165 (MQ=255)                                                                                                                                                                                      ttACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTa                                      >  1:478809/1‑251 (MQ=255)                                                                                                                                                                                           gAATAAGGTGTCGCGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGt                                                                                                                                                             <  1:553050/127‑1 (MQ=255)                                                                                                                                                                                            aaTAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCAc                                                                         >  1:203892/1‑210 (MQ=255)                                                                                                                                                                                              tAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATtggtg                              >  1:354136/1‑251 (MQ=255)                                                                                                                                                                                                  gtgtCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAagag                                                                                                              >  1:504945/1‑167 (MQ=255)                                                                                                                                                                                                  gtgtCGGGCCAGTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCa                          >  1:201363/1‑251 (MQ=255)                                                                                                                                                                                                             gTCGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTaaaa                                                                                                                                                                                                                   <  1:253663/55‑1 (MQ=255)                                                                                                                                                                                                               cGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAacac             <  1:49662/251‑1 (MQ=255)                                                                                                                                                                                                               cGATAATAAAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAacac             <  1:769987/251‑1 (MQ=255)                                                                                                                                                                                                                  taataaAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGAt          >  1:75884/1‑251 (MQ=255)                                                                                                                                                                                                                     taaAAGCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGgag                                                                                          <  1:641837/168‑1 (MQ=255)                                                                                                                                                                                                                          gCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGATTAAGGAt   <  1:396111/250‑1 (MQ=255)                                                                                                                                                                                                                          gCATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGATTAAGGATa  >  1:452162/1‑251 (MQ=255)                                                                                                                                                                                                                           cATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGATTAAGGAt   <  1:839458/249‑1 (MQ=255)                                                                                                                                                                                                                           cATAACGGGCGCCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGATTAAGGAt   <  1:24576/249‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CGAACAAAAACTTCCGTATCCACTAATAATAAGGTGTTGCTAATGGTCGCAAGAATAACGGTCAGACCGCGAAAAGTGCGGTGGTTTTTGACGATAAAACTGAGGGGGAAAATCACCAGCAGATAAAAGGCGAAAACAATAAAACTGAAATGCCCTAATAAACTGATG‑AAAAAATACACTTTACCGAATAAGGTGTCGGGCCAGTCGATAATAAAAGCATAACGGGCACCGATAAAAATCGCCCACAGAATATTAAAAAAAGCAAACCAGTGCCCCCACGAAATTTTTTGCGAGGTTTCTTCTCGATATTGTTTGCTGTTAAATTTCCACATAATGCTATTGGTTCGTCGTATTAAGAGAGTTCAGCAAAGCGTCGGAGAAGGCTTTTGCCAGCACTTGTCGTTGCTGTTTTCCGACACTGCCGAGCAGTAAATTGGTGGTCATATTGCCTAACACGATTAAGGATA  >  1/784191‑784657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑