Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	760,117	A→G	L171S (TTA→TCA)	unknown ←	Hypothetical lipoprotein YajG precursor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	760,117	0	A	G	100.0%	162.0 / NA	54	L171S (TTA→TCA)	unknown	Hypothetical lipoprotein YajG precursor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

GGTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTAACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGCCTTTGC  >  1/759871‑760348                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        ggTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGaca                                                                                                                                                                                                                                      >  1:513071/1‑250 (MQ=255) ggTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGaca                                                                                                                                                                                                                                      >  1:507976/1‑250 (MQ=255)  gTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGacac                                                                                                                                                                                                                                     <  1:420564/250‑1 (MQ=255)  gTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGacac                                                                                                                                                                                                                                     <  1:285510/250‑1 (MQ=255)  gTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGaca                                                                                                                                                                                                                                      >  1:40337/1‑249 (MQ=255)        gTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGa                                                                                                                                                                                                                              >  1:89310/1‑251 (MQ=255)                     agCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAAttt                                                                                                                                                                                                                  >  1:608954/1‑250 (MQ=255)                           aTTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGa                                                                                                                                                                                                           >  1:292702/1‑251 (MQ=255)                                         cTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAg                                                                                                                                                                                             <  1:464540/251‑1 (MQ=255)                                             gAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGaaaaa                                                                                                                                                                                                       <  1:494578/237‑1 (MQ=255)                                             gAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAgtgcg                                                                                                                                                                                         >  1:808848/1‑251 (MQ=255)                                                              tttttttCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTtata                                                                                                                                                                        <  1:311089/251‑1 (MQ=255)                                                               ttttttCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTatat                                                                                                                                                                       <  1:205453/251‑1 (MQ=255)                                                                 ttttCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATAttt                                                                                                                                                                     <  1:704621/251‑1 (MQ=255)                                                                        gTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAgtg                                                                                                                                                                                           >  1:11580/1‑222 (MQ=255)                                                                          tttACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAAt                                                                                                                                                            <  1:350528/251‑1 (MQ=255)                                                                                        tgGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTGAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGAc                                                                                                                                               <  1:587287/250‑1 (MQ=255)                                                                                              cAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAAc                                                                                                                                        <  1:253843/251‑1 (MQ=255)                                                                                                      aaaaCACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATgg                                                                                                                                <  1:558885/251‑1 (MQ=255)                                                                                                                              cTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCgg                                                                                                                                                                                               <  1:73481/164‑1 (MQ=255)                                                                                                                              cTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGAcc                                                                                                                                                       <  1:809923/204‑1 (MQ=255)                                                                                                                                 ttAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGt                                                                                                     <  1:675749/251‑1 (MQ=255)                                                                                                                                      cGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAGTTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGt                                                                                                >  1:554465/1‑251 (MQ=255)                                                                                                                                            gTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTtat                                                                                                                                                                         >  1:557937/1‑172 (MQ=255)                                                                                                                                                      ttttAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAg                                                                                                                                                                                             <  1:398099/142‑1 (MQ=255)                                                                                                                                                               aGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCAt                                                                       >  1:815562/1‑251 (MQ=255)                                                                                                                                                                            gCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAat                                                          >  1:144022/1‑251 (MQ=255)                                                                                                                                                                                aTTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTatat                                                                                                                                                                       <  1:484347/138‑1 (MQ=255)                                                                                                                                                                                   gATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGAc                                                                                                                                               <  1:149357/159‑1 (MQ=255)                                                                                                                                                                                     ttAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGt                                                                                                     <  1:398228/199‑1 (MQ=255)                                                                                                                                                                                     ttAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGt                                                 >  1:622314/1‑251 (MQ=255)                                                                                                                                                                                     ttAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGt                                                 >  1:430643/1‑251 (MQ=255)                                                                                                                                                                                           gCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACgg                                            >  1:90661/1‑250 (MQ=255)                                                                                                                                                                                              ttAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGgcagc                                        <  1:585503/251‑1 (MQ=255)                                                                                                                                                                                                     cTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGaa                                 >  1:167255/1‑251 (MQ=255)                                                                                                                                                                                                       tCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAAtt                               >  1:247304/1‑251 (MQ=255)                                                                                                                                                                                                        ccTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTg                              <  1:668625/251‑1 (MQ=255)                                                                                                                                                                                                           ggTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATgg                                                                                                                                <  1:588443/150‑1 (MQ=255)                                                                                                                                                                                                                ttATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCg                                                                                                 >  1:215985/1‑176 (MQ=255)                                                                                                                                                                                                                ttATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCAt                      >  1:200289/1‑251 (MQ=255)                                                                                                                                                                                                                ttATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCAt                      >  1:687889/1‑251 (MQ=255)                                                                                                                                                                                                                        tGATTCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATa               >  1:587198/1‑250 (MQ=255)                                                                                                                                                                                                                           ttCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAatat                                                        >  1:525216/1‑206 (MQ=255)                                                                                                                                                                                                                           ttCCACCACTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGa           <  1:242494/251‑1 (MQ=255)                                                                                                                                                                                                                              caccacTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGc        <  1:662677/251‑1 (MQ=255)                                                                                                                                                                                                                                   cTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGCCTTTg   >  1:877174/1‑251 (MQ=255)                                                                                                                                                                                                                                   cTTCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGCCTTTg   >  1:422868/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttCATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGCCTTTGc  <  1:97672/251‑1 (MQ=255)                                                                                                                                                                                                                                      cATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTatat                                                                                                                                                                       >  1:652224/1‑84 (MQ=255)                                                                                                                                                                                                                                      cATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCt                                                                                                                                             <  1:743467/110‑1 (MQ=255)                                                                                                                                                                                                                                      cATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGaa                                                                                                                                   <  1:544403/120‑1 (MQ=255)                                                                                                                                                                                                                                      cATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGc                                                                                                                 <  1:539783/138‑1 (MQ=255)                                                                                                                                                                                                                                      cATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAAt                                                                                            >  1:24144/1‑159 (MQ=255)                                                                                                                                                                                                                                      cATTAAAGGTTTCGCCTGACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGCCTTTg   >  1:603463/1‑248 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        GGTTCAAGTCCAGCAGGAGGAGCCAAATTCGAGAAAGCCGCTAAGAAATTAGCGGCTTTTGTTTTTTTCCGGTTTTACGCTTTTGTGTGGGGGCAAATTAAAAAACACCGGAAAAATCTACCGCACTTTTAAGCGTAAAGTGCGGTGGATTTTAGAAAAGTTTTTTAGTTGGCGTATTGATTAATGGCGTTAGCAACTTCCTGGTCTTTATAAATTGATTCCACCACTTCATTAAAGGTTTCGCCTAACACTTTTTGAATTTCCGAATTTCGCGCGAAAAATGCACCGGAGTGCGTGCGCCCGGCATTTATATTTTTCGTGAATTGACCTCTGCGACCTTGAACATGAATGGCAAGCTGAATTTTGCTGTCAATGTCGTAGCGTAAATTGCCTTGATTCACTTTGGCATAAAATTGCTGAATATCTACGGTGACGGCAGCATTGGAATTGTTCGCCATGCCGATACGGAAGCCTTTGC  >  1/759871‑760348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑