Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	711,779	T→C	V21A (GTA→GCA)	unknown →	SSU ribosomal protein S11p (S14e)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	711,779	0	T	C	100.0%	189.2 / NA	65	V21A (GTA→GCA)	unknown	SSU ribosomal protein S11p (S14e)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/25);  total (40/25)

AAGGGGATTTACGTCGTGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGTACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTAAAAGGACCAGGTCCTGGTCGAGA  >  1/711542‑711998                                                                                                                                                                                                                                              |                                                                                                                                                                                                                            aaGGGGATTTACGTCGTGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGc                                                                                                                                                                                                                 <  1:72428/250‑1 (MQ=255) aaGGGGATTTACGTCGTGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGCACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGc                                                                                                                                                                                                                 <  1:25684/250‑1 (MQ=255)      gATTTACGTCGTGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCttt                                                                                                                                                                                                           >  1:394104/1‑251 (MQ=255)          tACGTCGTGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCaat                                                                                                                                                                                                       <  1:12789/251‑1 (MQ=255)                gtGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACa                                                                                                                                                                                                 >  1:762605/1‑251 (MQ=255)                 tGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACaa                                                                                                                                                                                                <  1:555814/251‑1 (MQ=255)                   aaGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAAt                                                                                                                                                                                               >  1:287081/1‑250 (MQ=255)                   aaGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTA‑CCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCg                                                                                                                                                                                             >  1:185347/1‑251 (MQ=255)                   aaGTAACACTTAACATTAAACACCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAAt                                                                                                                                                                                               >  1:186936/1‑250 (MQ=255)                         cacTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTa                                                                                                                                                                                          >  1:602450/1‑249 (MQ=255)                           cTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACtat                                                                                                                                                                                      <  1:328694/251‑1 (MQ=255)                           cTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACtat                                                                                                                                                                                      <  1:758886/251‑1 (MQ=255)                               aCATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATaa                                                                                                                                                                                    >  1:330250/1‑249 (MQ=255)                               aCATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATaa                                                                                                                                                                                    >  1:797060/1‑249 (MQ=255)                                         ccTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAg                                                                                                                                                                        >  1:530070/1‑251 (MQ=255)                                            aTTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTa                                                                                                                                                                     >  1:41163/1‑251 (MQ=255)                                                gATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGc                                                                                                                                                                                                                 <  1:670135/203‑1 (MQ=255)                                                gATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGAc                                                                                                                                                                               >  1:876167/1‑237 (MQ=255)                                                gATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGC‑AAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCa                                                                                                                                                                >  1:617263/1‑251 (MQ=255)                                                 aTTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGAGTAAATTAAGATGGCAAAATCACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCa                                                                                                                                                                <  1:641075/251‑1 (MQ=255)                                                      ggTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACtat                                                                                                                                                                                      >  1:83832/1‑224 (MQ=255)                                                        ttgttATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCaa                                                                                                                                                                         >  1:712098/1‑235 (MQ=255)                                                             aTCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTggg                                                                                                                                                      >  1:703812/1‑249 (MQ=255)                                                             aTCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTggg                                                                                                                                                      >  1:389056/1‑249 (MQ=255)                                                                  ggTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTActg                                                                                                                                                >  1:241513/1‑250 (MQ=255)                                                                   gTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTActg                                                                                                                                                >  1:507513/1‑249 (MQ=255)                                                                                tcgtAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGtt                                                                                                                                 <  1:304078/251‑1 (MQ=255)                                                                                     gTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGt                                                                                                                            >  1:532758/1‑251 (MQ=255)                                                                                        tACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTg                                                                                                                                                                                 <  1:263461/195‑1 (MQ=255)                                                                                            ggTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCaat                                                                                                                                                                                                       >  1:693166/1‑169 (MQ=255)                                                                                                    gTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAAt                                                                                                               >  1:68435/1‑249 (MQ=255)                                                                                                        aCGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTAccc                                                                                                         <  1:341458/251‑1 (MQ=255)                                                                                                           tACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTg                                                                                                                                                                                 >  1:284595/1‑176 (MQ=255)                                                                                                                aaaCTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAgg                                                                                                                                                                       >  1:121241/1‑181 (MQ=255)                                                                                                                   cTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATAc                                                                                                                                                                                                  <  1:565946/151‑1 (MQ=255)                                                                                                                             tACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCgg                                                                                    >  1:485525/1‑251 (MQ=255)                                                                                                                             tACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGACGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTaaa                                                                                                                <  1:453910/223‑1 (MQ=255)                                                                                                                               cccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGc                                                                                                                                                                                                                 <  1:510474/124‑1 (MQ=255)                                                                                                                               cccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGc                                                                                                                                                           <  1:550043/178‑1 (MQ=255)                                                                                                                               cccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCt                                                                                                                                                          >  1:324455/1‑179 (MQ=255)                                                                                                                               cccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTg                                                                                                                           >  1:100594/1‑210 (MQ=255)                                                                                                                               cccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCa                                                                                  >  1:27185/1‑251 (MQ=255)                                                                                                                                ccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGt                                                                                                                                                                      >  1:489018/1‑166 (MQ=255)                                                                                                                                ccGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAg                                                                                 >  1:677324/1‑251 (MQ=255)                                                                                                                                          ccTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAgg                                                                                          >  1:313040/1‑232 (MQ=255)                                                                                                                                           cTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTgg                                                                                                                          >  1:15116/1‑199 (MQ=255)                                                                                                                                           cTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTtgtg                                                                        >  1:876266/1‑249 (MQ=255)                                                                                                                                           cTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTtgtg                                                                        >  1:770049/1‑249 (MQ=255)                                                                                                                                            tCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTg                                                                     <  1:261427/251‑1 (MQ=255)                                                                                                                                                      aTCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAgg                                                                                                                                   <  1:410879/179‑1 (MQ=255)                                                                                                                                                       tCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCaat                                                                                                                                                                                                       <  1:230557/110‑1 (MQ=255)                                                                                                                                                        cAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAg                                                         <  1:16259/251‑1 (MQ=255)                                                                                                                                                                     gggTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTActgc                                                                                                                                               >  1:571448/1‑152 (MQ=255)                                                                                                                                                                      ggTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCt                                                                                                                                                          >  1:484033/1‑140 (MQ=255)                                                                                                                                                                       gTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAg                                           <  1:19362/250‑1 (MQ=255)                                                                                                                                                                         aaaTTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGc                                                                                   >  1:514065/1‑208 (MQ=255)                                                                                                                                                                                 aTGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGTTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAg                                 <  1:172805/250‑1 (MQ=255)                                                                                                                                                                                        aaaCACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTa                         <  1:695284/251‑1 (MQ=255)                                                                                                                                                                                                gTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTc                                                                                                    <  1:704463/168‑1 (MQ=255)                                                                                                                                                                                                gTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGCTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTAAAAGGAc                  >  1:880228/1‑250 (MQ=255)                                                                                                                                                                                                 ttCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTAAAAGGAcc                 <  1:159007/250‑1 (MQ=255)                                                                                                                                                                                                     tGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTAAAAGGACCAGGt             <  1:396384/250‑1 (MQ=255)                                                                                                                                                                                                               cGTGTAAAAAAACAAGTTGTAGATGGCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTAAAAGGACCAGGTCCTGGTCgaga  >  1:563165/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAAc                                                                                                                                                                                   >  1:227045/1‑49 (MQ=255)                                                                                                                                                                                                                                        gCGTTGCACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGtt                                                                                        >  1:502573/1‑140 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                            AAGGGGATTTACGTCGTGAAGTAACACTTAACATTAAACGCCTATTAGATTTAGGTTGTTATCGTGGTTTACGTCATCGTCGTAGTTTACCGGTACGTGGTCAACGTACTAAAACTAATGCGCGTACCCGTAAGGGTCCTCGTAAGCCGATCAAAAAATAGTCGGGGTAAATTAAGATGGCAAAAACACCAGTTCGTGCACGTAAACGTGTAAAAAAACAAGTTGTAGATGGCGTTGTACACATTCACGCATCTTTCAATAATACAATCGTTACTATAACTGACCGCCAAGGTAATGCATTAGCTTGGGCTACTGCTGGTGGTTCAGGTTTCCGTGGTTCTCGTAAATCTACCCCGTTCGCTGCACAGGTTGCGGCAGAACGTTGTGCTGAAATTGTAAAAGAATTCGGCTTAAAGAACTTGGAAGTTATGGTAAAAGGACCAGGTCCTGGTCGAGA  >  1/711542‑711998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑