Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	104,673	A→G	A81A (GCT→GCC)	unknown ←	D‑glycerate transporter (predicted)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	104,673	0	A	G	98.6%	189.8 / ‑4.5	69	coding (243/1275 nt)	unknown	D‑glycerate transporter (predicted)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (48/20);  minor base . (0/1);  total (48/21)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCAGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCGCCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAA  >  1/104434‑104897                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                   gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:27904/1‑250 (MQ=255) gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:644311/1‑250 (MQ=255) gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:803116/1‑250 (MQ=255) gCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         >  1:486863/1‑250 (MQ=255) gCATTGGGGTTCGGCGACAAAATATTCCCCGCTTTCCCCCCCCCGATCACCGCCAACAAAATCGCCGTTTTGGATAAAT‑CGGTACGACGCGCCAGGGCAAGGGCAAT‑CGG‑TGATACGTTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aat                                                                                                                                                                                                                         <  1:127505/250‑1 (MQ=255)      ggggTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGAAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aatcaat‑c                                                                                                                                                                                                                   <  1:406691/250‑1 (MQ=255)          ttCGGCGACATAATATTCCCCGCTTTCCCACCCCCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCAAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               <  1:81940/250‑1 (MQ=255)                 aCATAATATTCCCCGCTTTAACACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCAcgcccgc                                                                                                                                                                                                        >  1:836146/1‑250 (MQ=255)                   ataataTTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCaa                                                                                                                                                                                                     >  1:118225/1‑251 (MQ=255)                    taataTTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCaa                                                                                                                                                                                                     >  1:764437/1‑250 (MQ=255)                    aaaattcccccGCTTTCCCCCCCCCGATCATCGCCAGAAAAATCGCCGGTTTGGAAAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATCCGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAac                                                                                                                                                                                                    <  1:309794/244‑1 (MQ=255)                                cTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACcggcgg                                                                                                                                                                                          >  1:901697/1‑249 (MQ=255)                                   tccccccaccGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGAAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               <  1:745350/220‑1 (MQ=255)                                   tACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTaaa                                                                                                                                                                                     >  1:603451/1‑251 (MQ=255)                                      cccccccgtccatcccaaaaaaattCGCCGGTTTGGATAAAT‑CGTAACGCCCCGCAAAGGCAGGGGCAAT‑GGG‑TGATACGGAAATCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATc                                                                                                                                                                                  <  1:616837/227‑1 (MQ=255)                                                          aaaacccccGGTTTTGAAAAATCCGGTCCGCCGCCCCAAGG‑AAGGGCAATCCGG‑TGACCCGGAAATCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTaa                                                                                                                                                               <  1:26495/244‑1 (MQ=255)                                                                    gTTTGGATAAAT‑CGGTACGACGCGCAAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACAcc                                                                                                                                                                                               <  1:784030/208‑1 (MQ=255)                                                                             aaT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGAt                                                                                                                                            >  1:847149/1‑250 (MQ=255)                                                                              aagcggtccgccgcgCCAAGGAAAGGGCAAT‑GGG‑TAATAGGGAAACCC‑CCCCCACAACAACGAACCCCCCAAAAGGTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAAGGCCAACAAAGCCC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATc                                                                                                                                           <  1:83128/240‑1 (MQ=255)                                                                               acccggaccgccgcccAAGGGAAGGGCAAT‑TGG‑TTAATCGGTAAACCCCCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTCCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATc                                                                                                                                           <  1:12690/237‑1 (MQ=255)                                                                                   gTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTa                                                                                                                                                                >  1:262721/1‑225 (MQ=255)                                                                                     ccgccgccccAAGGCAGGGGAATT‑GGG‑TGATCCGGTACCCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGAc                                                                                                                                    <  1:767/243‑1 (MQ=255)                                                                                       cgccgcccAAGGGAAGGGGAAT‑GGGTTGATACGGAAACCC‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACt                                                                                                                                   <  1:54930/245‑1 (MQ=255)                                                                                                 ggcaaggGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGAc                                                                                                                                    >  1:173085/1‑239 (MQ=255)                                                                                                 ggcaaggGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCa                                                                                                                        >  1:421500/1‑251 (MQ=255)                                                                                                      gggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGccc                                                                                                                                                          >  1:1144/1‑212 (MQ=255)                                                                                                       ggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑aatcaat‑c                                                                                                                                                                                                                   >  1:832117/1‑154 (MQ=255)                                                                                                       ggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACcgg                                                                                                                                                                                             >  1:234567/1‑176 (MQ=255)                                                                                                       ggCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAAt                                                                                                                  >  1:695224/1‑251 (MQ=255)                                                                                                              cGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               >  1:668853/1‑152 (MQ=255)                                                                                                              atgggtaatcggtaaacccccGCCACATCACCGAACACGCAAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCAAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAACGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGc                                                                                                              <  1:787945/232‑1 (MQ=255)                                                                                                                g‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCAccg                                                                                                          >  1:830781/1‑251 (MQ=255)                                                                                                                g‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCAccg                                                                                                          >  1:537007/1‑251 (MQ=255)                                                                                                                g‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCAccg                                                                                                          >  1:208567/1‑251 (MQ=255)                                                                                                                      aCGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGa                                                                                                      >  1:660403/1‑250 (MQ=255)                                                                                                                      aCGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGa                                                                                                      >  1:26523/1‑250 (MQ=255)                                                                                                                       cGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGcc                                                                                                                                                           >  1:577387/1‑196 (MQ=255)                                                                                                                        ggTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCa                                                                                                                                                                                                               >  1:168974/1‑143 (MQ=255)                                                                                                                         gTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGc                                                                                                                                                            >  1:426815/1‑193 (MQ=255)                                                                                                                         gTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCaa                                                                                                  >  1:393208/1‑251 (MQ=255)                                                                                                                                       caTCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTaa                                                                                      >  1:11406/1‑250 (MQ=255)                                                                                                                                         tCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACg                                                                                    >  1:685265/1‑250 (MQ=255)                                                                                                                                          cAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGc                                                                                                                                                            >  1:594370/1‑177 (MQ=255)                                                                                                                                                   cGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAg                                                                          >  1:869328/1‑250 (MQ=255)                                                                                                                                                      ccaactcctgtaaaaaccttcgtcgCA‑AAGGC‑TAATCCCACCAAGGCCC‑GGGCTCCCCCCAAC‑TTATGGGAATTGGTTC‑CCGCAA‑CCTTGTTGGC‑GCCCCCG‑AAACAATCCACCCCGCCCGCCACCACCCCGGCGGCTAAAACCCGCATGCCCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCAt                                                                       <  1:91509/232‑1 (MQ=255)                                                                                                                                                       aaCAGCTTTAAAAA‑TCATCGCCGC‑‑AAGGCATAATGCAAACAAGGC‑G‑GGGGTTCCCCCCACTTTATGGGTAA‑GGTTTCCCGCAA‑TCGTGTGGGCGGCACGGA‑AAACAAT‑CAGCACGCCCGCCAACACCCGGGCGCCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACAAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATAc                                                                     <  1:574478/251‑1 (MQ=255)                                                                                                                                                        aacggctttAAAACTCATCGTCGAA‑AGGGC‑TAATGCCAACAAAGCAC‑GGGCTCCCCCCACT‑TTATTGGAAATGGTTC‑CCGCAA‑TGGTGTGGGCGGCCCCGA‑AACCAAT‑CAGCCCGCCCGCCACCACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATAc                                                                     <  1:857085/244‑1 (MQ=255)                                                                                                                                                         caacTGTTAAAA‑TCTTCGTC‑CTGAAGAC‑TAATGCCAAC‑AAGCCCGGGGCTTCCCCCAAG‑TT‑TTAGGAATTGTTT‑TCGAAATTGGTTTTGGGGGCACCGG‑AATAAAA‑CAGCACCCCCGCCAACACACCGGCGGCAAAAACCCGCTTGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCg                                                                   <  1:230556/248‑1 (MQ=255)                                                                                                                                                            cTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTaa                                                                                      >  1:100793/1‑229 (MQ=255)                                                                                                                                                              gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGc                                                                         >  1:34620/1‑240 (MQ=255)                                                                                                                                                              gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGt                                                                  >  1:113706/1‑247 (MQ=255)                                                                                                                                                              gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATg                                                               >  1:619568/1‑250 (MQ=255)                                                                                                                                                              gTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATg                                                               >  1:176746/1‑250 (MQ=255)                                                                                                                                                               taaaaa‑TCACCGTCGCA‑AGGGC‑TAATCCCAACAAAGCAC‑GGGCTCCCCCAAAC‑TTATGGGTAAGGGTTC‑CCGCAA‑TCGTG‑TGGGGGCCCCGGAAATCAAC‑CAGCCCGCCCGCAAACACCCGGGCGGCTAAAACCCGCATGCCCGCCGTGGTAATGCCCTGCGCCCCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGcc                                                             <  1:898287/249‑1 (MQ=255)                                                                                                                                                                              gCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGAc                                                                                                                                    >  1:529339/1‑166 (MQ=255)                                                                                                                                                                                    ggC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACgg                                                                                                                              >  1:214370/1‑167 (MQ=255)                                                                                                                                                                                           tGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAac                                                                                                                                                                                                    <  1:615312/91‑1 (MQ=255)                                                                                                                                                                                              caacaaAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCaagag                                   >  1:539886/1‑249 (MQ=255)                                                                                                                                                                                                           ggCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCaa                     >  1:507204/1‑251 (MQ=255)                                                                                                                                                                                                               tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATg                                                                                                                                        <  1:856050/132‑1 (MQ=255)                                                                                                                                                                                                               tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTc                                              >  1:109904/1‑222 (MQ=255)                                                                                                                                                                                                               tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:482206/1‑250 (MQ=255)                                                                                                                                                                                                               tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:334875/1‑250 (MQ=255)                                                                                                                                                                                                               tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:282353/1‑250 (MQ=255)                                                                                                                                                                                                               tCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTg                  >  1:276536/1‑250 (MQ=255)                                                                                                                                                                                                                     aaC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAAccgc                                                                                             >  1:852369/1‑169 (MQ=255)                                                                                                                                                                                                                            ttGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCGTTTCCTTTTTCAAAAACAGGAGGATCGCCACCGCAAGTGCAATGACCGCTc      >  1:805640/1‑250 (MQ=255)                                                                                                                                                                                                                               gTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCAccgc                                                                                                                                                <  1:116516/109‑1 (MQ=255)                                                                                                                                                                                                                               gTAATGGTTT‑CCGCAA‑TCGTGTTGGCGGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCACCAGCATACCGTATGCCGGCTTCCCTTTTTACAAAATCAGTAGGATCGCCACCGCAAGTGCAATGACCGCTCCCaa  >  1:746923/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                   GCATTGGGGTTCGGCGACATAATATTCCCCGCTTTACCACCACCGATCATCGCCAGCAAAATCGCCGGTTTGGATAAAT‑CGGTACGACGCGCCAAGGCAAGGGCAAT‑CGG‑TGATACGGTAATCA‑CCGCCACATCAACGAACACGCCAACAGCTGTTAAAA‑TCATCGTCGCT‑AAGGC‑TAATGCCAACAAAGCAC‑GGGCTTCCCCCAAC‑TTATTGGTAATGGTTT‑CCGCAA‑TCGTGTTGGCAGCACCGG‑AATCAAT‑CAGCACGCCCGCCAACACACCGGCGGCTAAAATCCGCATGACCGCCGTGGTAATGCCCTGCGCACCGCCGATCATGAGACTCACGGTTTGCACTAAATCGGCACCGCCGACCAAACCGCCGATTAACGCCCCCGCCAGCATACCGTATGCCGGCGATACTTTTTTCAAAATCAGGAGGATCGCCACCGCAAGTGCAATGACCGCTCCCAA  >  1/104434‑104897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑