Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	709,956	A→G	S73G (AGC→GGC)	unknown →	Protein translocase subunit SecY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	709,956	0	A	G	100.0%	207.9 / NA	71	S73G (AGC→GGC)	unknown	Protein translocase subunit SecY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/35);  total (36/35)

TTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGAGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTGGTTCCTAATTTGGGATT  >  1/709721‑710194                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               ttCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAAtt                                                                                                                                                                                                                                  <  1:129691/250‑1 (MQ=255)       cGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTgcgc                                                                                                                                                                                                                           <  1:147557/251‑1 (MQ=255)                attaatGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGtatta                                                                                                                                                                                                                  >  1:710964/1‑251 (MQ=255)                 ttaatGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGtattat                                                                                                                                                                                                                 <  1:611214/251‑1 (MQ=255)                   aatGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGc                                                                                                                                                                                                               <  1:18972/251‑1 (MQ=255)                   aatGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTGTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGc                                                                                                                                                                                                               <  1:480313/251‑1 (MQ=255)                          aaaCAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTatat                                                                                                                                                                                                        >  1:2678/1‑251 (MQ=255)                                              aagaagTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGGTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATcc                                                                                                                                                                                    <  1:450112/251‑1 (MQ=255)                                                gaagTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCaa                                                                                                                                                                                  >  1:332580/1‑251 (MQ=255)                                                   gTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTg                                                                                                                                                                               >  1:205891/1‑251 (MQ=255)                                                     aCTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTatat                                                                                                                                                                                                        <  1:577203/224‑1 (MQ=255)                                                               gtACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGtattat                                                                                                                                                                                                                 >  1:728487/1‑205 (MQ=255)                                                               gtACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAatta                                                                                                                                                                                          <  1:739183/228‑1 (MQ=255)                                                               gtACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGt                                                                                                                                                                    <  1:592413/250‑1 (MQ=255)                                                                                    gATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGtat                                                                                                                                                                                                                    <  1:667894/181‑1 (MQ=255)                                                                                        gTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGc                                                                                                                                                                                                               >  1:200681/1‑182 (MQ=255)                                                                                        gTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTatat                                                                                                                                                                                                        <  1:216781/189‑1 (MQ=255)                                                                                         ttATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTaagaaa                                                                                                                                         <  1:402643/251‑1 (MQ=255)                                                                                               gTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTgcgc                                                                                                                                                                                                                           >  1:370122/1‑163 (MQ=255)                                                                                                         cACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGc                                                                                                                                                                                                               >  1:416714/1‑165 (MQ=255)                                                                                                         cACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGgt                                                                                                                         <  1:207253/251‑1 (MQ=255)                                                                                                                   tttCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGa                                                                                                               <  1:350964/251‑1 (MQ=255)                                                                                                                    ttCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGAt                                                                                                              <  1:500568/251‑1 (MQ=255)                                                                                                                         aaTTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTa                                                                                                         <  1:736974/251‑1 (MQ=255)                                                                                                                          aTTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTaa                                                                                                        <  1:350441/251‑1 (MQ=255)                                                                                                                          aTTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTaa                                                                                                        <  1:445871/251‑1 (MQ=255)                                                                                                                                 cattcattCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATCTGTTTAATATGTTCTCTGGCGGTGCGTTGGGCCGGGCCTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATAc                                                                                                  >  1:577099/1‑250 (MQ=255)                                                                                                                                     cattcCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGtt                                                                                             >  1:506205/1‑251 (MQ=255)                                                                                                                                       ttcCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTAt                                                                                           >  1:665940/1‑251 (MQ=255)                                                                                                                                              tCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTaa                                                                                                        <  1:347057/231‑1 (MQ=255)                                                                                                                                                cTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGc                                                                                                                                                                                                               >  1:801871/1‑126 (MQ=255)                                                                                                                                                   gTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTaagaaaaga                                                                                                                                      >  1:304167/1‑196 (MQ=255)                                                                                                                                                   gTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTtgtg                                                                               >  1:645546/1‑251 (MQ=255)                                                                                                                                                   gTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTtgtg                                                                               >  1:513513/1‑251 (MQ=255)                                                                                                                                                   gTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTtgtg                                                                               >  1:132795/1‑251 (MQ=255)                                                                                                                                                                 gTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTtat                                                                                                                                                                                                          >  1:720763/1‑114 (MQ=255)                                                                                                                                                                 gTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTc                                                                                                                             >  1:675036/1‑191 (MQ=255)                                                                                                                                                                 gTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGa                                                                                                               >  1:270803/1‑205 (MQ=255)                                                                                                                                                                 gTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAatat                                                                                                    <  1:461225/216‑1 (MQ=255)                                                                                                                                                                 gTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACa                                                                 >  1:762369/1‑251 (MQ=255)                                                                                                                                                                     tAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCg                                                             <  1:399381/251‑1 (MQ=255)                                                                                                                                                                       gCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGc                                                                                                                                                                          >  1:438712/1‑140 (MQ=255)                                                                                                                                                                                        acaaAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCt                                          <  1:602143/251‑1 (MQ=255)                                                                                                                                                                                             aaGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCt                                     <  1:680663/251‑1 (MQ=255)                                                                                                                                                                                                  aCCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAatat                                <  1:34173/251‑1 (MQ=255)                                                                                                                                                                                                   ccATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTaagaaa                                                                                                                                         >  1:607795/1‑145 (MQ=255)                                                                                                                                                                                                     atcatTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACgg                                                                                    <  1:272050/196‑1 (MQ=255)                                                                                                                                                                                                      tcatTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTAc                                                                       <  1:673085/208‑1 (MQ=255)                                                                                                                                                                                                      tcatTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCg                                                             <  1:755412/218‑1 (MQ=255)                                                                                                                                                                                                      tcatTGATATGTTTAATATGTTCTCTGGTAGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAAATGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATAc                                                                                                  <  1:549337/181‑1 (MQ=255)                                                                                                                                                                                                         ttGATATGTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCt                         <  1:257117/251‑1 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATAc                                                                                                  <  1:582812/171‑1 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAgg                                                               >  1:86119/1‑206 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTAcc                          >  1:81156/1‑243 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTAc                           >  1:668970/1‑242 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTgg                  >  1:199021/1‑251 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTgg                  >  1:794686/1‑251 (MQ=255)                                                                                                                                                                                                                gTTTAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAACTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGtgc                                                                              >  1:336197/1‑191 (MQ=255)                                                                                                                                                                                                                  ttAATATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATAcc                                                                                                 >  1:64707/1‑170 (MQ=255)                                                                                                                                                                                                                    aaTATGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGAt                                                                                                              <  1:381478/155‑1 (MQ=255)                                                                                                                                                                                                                        tGTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTAc                                                                  <  1:274267/195‑1 (MQ=255)                                                                                                                                                                                                                         gTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACgg                                                                                    >  1:571502/1‑176 (MQ=255)                                                                                                                                                                                                                         gTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATg                               >  1:898897/1‑229 (MQ=255)                                                                                                                                                                                                                         gTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTAcc                          <  1:436664/234‑1 (MQ=255)                                                                                                                                                                                                                         gTTCTCTGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTGGTTCCTAAtt         >  1:436763/1‑251 (MQ=255)                                                                                                                                                                                                                            ctctGGTGGTGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAatat                                <  1:709648/225‑1 (MQ=255)                                                                                                                                                                                                                                ggtggtGCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATc                                                                                                                                                                                     >  1:76558/1‑72 (MQ=255)                                                                                                                                                                                                                                 gtggtgCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTGGTTCCTAATTTGGGAtt  >  1:65468/1‑250 (MQ=255)                                                                                                                                                                                                                                 gtggtgCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTGGTTCCTAATTTGGGAtt  <  1:56367/250‑1 (MQ=255)                                                                                                                                                                                                                                 gtggtgCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTGGTTCCTAATTTGGGAtt  >  1:541136/1‑250 (MQ=255)                                                                                                                                                                                                                                      gCGTTGGGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGa                                                                                                               >  1:168907/1‑136 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               TTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGGTGGTACAAGTGAGCTAAAGAGCAGATTGTTATTTGTTTTAGGTGCACTTATTGTTTTCCGAATTGGTTCATTCATTCCGGTTCCTGGTATAGACGCGGCTGTATTAGCTCAGTTGATTGAACAACAAAAAGGCACCATCATTGATATGTTTAATATGTTCTCTGGTGGTGCGTTGAGCCGGGCTTCAATTTTTGCGCTTGGTATTATGCCTTATATTTCGGCATCAATTATTATCCAATTGTTAGCTTCGGTTCATCCGGCACTTGCGGAATTAAGAAAAGAAGGTGAGTCAGGTCGTCGTAAGATTAGTAAATATACCCGTTATTCAACGGTTGTGCTTGCTACTCTACAGGCGGTAGGTATTTCAACTGGCTTGCCTAATATGTTACCTGGCTTGGTTCCTAATTTGGGATT  >  1/709721‑710194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑