Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	709,541	C→T	D80D (GAC→GAT)	unknown →	LSU ribosomal protein L15p (L27Ae)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	709,541	0	C	T	100.0%	170.9 / NA	60	D80D (GAC→GAT)	unknown	LSU ribosomal protein L15p (L27Ae)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/35);  total (25/35)

CGCTTAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGACGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGG  >  1/709305‑709780                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                cgcTTAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATaa                                                                                                                                                                                                                                      <  1:433193/248‑1 (MQ=255) cgcTTAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAAt                                                                                                                                                                                                                                   <  1:412839/251‑1 (MQ=255)   cTTAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAAt                                                                                                                                                                                                                                   <  1:758578/249‑1 (MQ=255)   cTTAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTg                                                                                                                                                                                                                                 >  1:628098/1‑251 (MQ=255)     tAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGaa                                                                                                                                                                                                                               >  1:582335/1‑251 (MQ=255)           ctctATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGt                                                                                                                                                                                                                         <  1:552955/251‑1 (MQ=255)           ctctATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGt                                                                                                                                                                                                                         >  1:17423/1‑251 (MQ=255)           ctctATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGt                                                                                                                                                                                                                         >  1:901119/1‑251 (MQ=255)                               gCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACAtt                                                                                                                                                                                                     >  1:322596/1‑251 (MQ=255)                                  aaGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACa                                                                                                                                                                                                       <  1:338856/246‑1 (MQ=255)                                     cATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTTGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGc                                                                                                                                                                                               <  1:426964/251‑1 (MQ=255)                                         gTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGAc                                                                                                                                                                                                                 <  1:331307/229‑1 (MQ=255)                                         gTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAg                                                                                                                                                                                                <  1:564267/246‑1 (MQ=255)                                         gTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGc                                                                                                                                                                                            >  1:329878/1‑250 (MQ=255)                                          tGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCa                                                                                                                                                                                          <  1:789756/251‑1 (MQ=255)                                                 cttcttGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATAtttt                                                                                                                                                                                   >  1:768516/1‑251 (MQ=255)                                                 cttcttGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATAtttt                                                                                                                                                                                   >  1:601329/1‑251 (MQ=255)                                                    cttGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAAc                                                                                                                                                                                <  1:305641/251‑1 (MQ=255)                                                          cGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGa                                                                                                                                                                          <  1:458699/251‑1 (MQ=255)                                                              gTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAg                                                                                                                                                                                                >  1:871384/1‑225 (MQ=255)                                                                 ttGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAg                                                                                                                                                                   <  1:107989/251‑1 (MQ=255)                                                                   ggTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTc                                                                                                                                                                     <  1:698411/247‑1 (MQ=255)                                                                   ggTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGtt                                                                                                                                                                 >  1:163631/1‑251 (MQ=255)                                                                    gTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATAttt                                                                                                                                                                                    <  1:611247/231‑1 (MQ=255)                                                                         ggTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTaa                                                                                                                                                           >  1:807418/1‑251 (MQ=255)                                                                                    aaCTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAg                                                                                                                                                <  1:578762/251‑1 (MQ=255)                                                                                                tGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGtt                                                                                                                                     <  1:528994/250‑1 (MQ=255)                                                                                                  gTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTAACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTaaa                                                                                                                                  >  1:445665/1‑251 (MQ=255)                                                                                                    cATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTaaaaa                                                                                                                                <  1:291081/251‑1 (MQ=255)                                                                                                                      cGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAAt                                                                                                                                                                                                                                   <  1:559590/134‑1 (MQ=255)                                                                                                                       gTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAAt                                                                                                                                                                                                                                        <  1:130780/128‑1 (MQ=255)                                                                                                                        tACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAg                                                                                                                        >  1:843360/1‑239 (MQ=255)                                                                                                                        tACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGt                                                                                                            <  1:704932/251‑1 (MQ=255)                                                                                                                        tACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGt                                                                                                            <  1:370198/251‑1 (MQ=255)                                                                                                                          cTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTTAGtt                                                                                                                                                                 <  1:731260/196‑1 (MQ=255)                                                                                                                            ggtggCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCg                                                                                                        >  1:468417/1‑251 (MQ=255)                                                                                                                                     gtTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTaa                                                                                               >  1:508538/1‑251 (MQ=255)                                                                                                                                       tcgtcgTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAg                                                                                             <  1:472560/251‑1 (MQ=255)                                                                                                                                        cgtcgtGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTCAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAgg                                                                                            <  1:624985/251‑1 (MQ=255)                                                                                                                                                gTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAAc                                                                                                                                                                                >  1:198393/1‑159 (MQ=255)                                                                                                                                                gTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGt                                                                                                            <  1:283202/227‑1 (MQ=255)                                                                                                                                                gTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAag                                                                                    >  1:223631/1‑251 (MQ=255)                                                                                                                                                        gtggtCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAAc                                                                                                                                                                                >  1:239719/1‑151 (MQ=255)                                                                                                                                                           gtCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAaaat                                                                                                                               <  1:149725/197‑1 (MQ=255)                                                                                                                                                                   ccTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAg                                                                                                                                                                                                <  1:822553/124‑1 (MQ=255)                                                                                                                                                                   ccTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGt                                                                                                            >  1:267150/1‑208 (MQ=255)                                                                                                                                                                   ccTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCtgg                                                                 >  1:524707/1‑251 (MQ=255)                                                                                                                                                                        atatCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGtt                                                            <  1:891639/251‑1 (MQ=255)                                                                                                                                                                             gtcgtTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAgc                                                                       <  1:630370/235‑1 (MQ=255)                                                                                                                                                                              tcgtTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGGTATTCAGTTTGCCAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCg                                                      <  1:694182/251‑1 (MQ=255)                                                                                                                                                                              tcgtTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCg                                                      >  1:157287/1‑251 (MQ=255)                                                                                                                                                                                      cTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGaataa                                              <  1:41910/251‑1 (MQ=255)                                                                                                                                                                                                          aTGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTg                                                                                                          <  1:275674/171‑1 (MQ=255)                                                                                                                                                                                                                       tAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACt                                                                                                                                                                               <  1:188070/89‑1 (MQ=255)                                                                                                                                                                                                                       tAACAGCAGAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACaa               <  1:498563/249‑1 (MQ=255)                                                                                                                                                                                                                          cagcagAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGt          >  1:610241/1‑251 (MQ=255)                                                                                                                                                                                                                          cagcagAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGt          >  1:372943/1‑251 (MQ=255)                                                                                                                                                                                                                            gcagAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCa                            >  1:277609/1‑231 (MQ=255)                                                                                                                                                                                                                            gcagAAGTCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTa         >  1:848937/1‑250 (MQ=255)                                                                                                                                                                                                                                   tCCGTCTAGATGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAgg  <  1:519632/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                CGCTTAAATACTCTATCTCCGGCTGAAGGTGCTAAGCATAGTGCTAAACTTCTTGGTCGTGGTATTGGTTCAGGTTTAGGTAAAACTGGTGGACGTGGTCATAAAGGTCAAAAATCTCGTACTGGTGGCGGTGTTCGTCGTGGGTTCGAAGGTGGTCAAATGCCTTTATATCGTCGTTTACCTAAATTTGGTTTTACTTCTATGAAATCTGCAGTAACAGCAGAAGTCCGTCTAGACGACCTGAATAAAATTGAAAATGGTATTGTGACTTTAGAAACATTAAAAGCTGCCAATATTTTAACTAAGGATATTCAGTTTGCTAAAGTAATTTTAGCGGGTGAAGTTAAAAATAAATTAGTTATTCGTGGGTTGCGTGTGACTAAAGGCGCCAAAGCAGCTATTGAAGCTGCTGGTGGTTCAATCGAGGAATAATTAATGGCTAAACAACCAGGCTATCAAACAAGAAGTACTCAAGG  >  1/709305‑709780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑