Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	705,395	C→T	intergenic (+34/‑288)	unknown → / → unknown	Transposase, IS200 family/LSU ribosomal protein L14p (L23e)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	705,395	0	C	T	100.0%	182.2 / NA	71	intergenic (+34/‑288)	unknown/unknown	Transposase, IS200 family/LSU ribosomal protein L14p (L23e)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/38);  total (33/38)

TGAAAGGCACGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCACATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTATGGGTAGGTT  >  1/705178‑705641                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       tGAAAGGCACGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGcc                                                                                                                                                                                                                       <  1:368928/251‑1 (MQ=2)        cACGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAg                                                                                                                                                                                                                <  1:815025/251‑1 (MQ=9)         aCGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAgg                                                                                                                                                                                                               <  1:73871/251‑1 (MQ=9)          cGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAggg                                                                                                                                                                                                              <  1:173763/251‑1 (MQ=12)            cAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGt                                                                                                                                                                                                             <  1:705236/250‑1 (MQ=12)              aaCCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAg                                                                                                                                                                                                           <  1:324872/250‑1 (MQ=16)                    aaaTACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTaaaa                                                                                                                                                                                                    >  1:671390/1‑251 (MQ=16)                           aCTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCAc                                                                                                                                                                                             <  1:496599/251‑1 (MQ=16)                              aTGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAg                                                                                                                                                                                                                <  1:596225/229‑1 (MQ=9)                                     ccGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTg                                                                                                                                                                                   <  1:743813/251‑1 (MQ=16)                                                        ggCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAg                                                                                                                                                                                                                >  1:809709/1‑203 (MQ=9)                                                        ggCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTg                                                                                                                                                                >  1:262100/1‑251 (MQ=255)                                                        ggCTATTATGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTg                                                                                                                                                                >  1:719766/1‑251 (MQ=255)                                                             ttatGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAggg                                                                                                                                                                                                              <  1:695603/200‑1 (MQ=255)                                                               atGTAAGCACGGTAGGGCTAAATACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAg                                                                                                                                                                                                                <  1:100932/196‑1 (MQ=255)                                                                                    aTACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAGGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAAc                                                                                                                                               >  1:705038/1‑240 (MQ=255)                                                                                    aTACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGc                                                                                                                                                                                                                        <  1:445742/167‑1 (MQ=255)                                                                                     tACAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAAtt                                                                                                                                    >  1:807770/1‑250 (MQ=255)                                                                                          aggtaGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTg                                                                                                                                                                                   <  1:346031/194‑1 (MQ=255)                                                                                            gtaGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACAt                                                                                                                                             <  1:704376/232‑1 (MQ=255)                                                                                             taGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCa                                                                                                                            >  1:121018/2‑250 (MQ=255)                                                                                               gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGcc                                                                                                                                                                                                                       >  1:813109/5‑157 (MQ=9)                                                                                               gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACtt                                                                                                                         >  1:54500/5‑251 (MQ=255)                                                                                               gtgtaggagTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTAGACCGCACtt                                                                                                                         >  1:432470/5‑251 (MQ=255)                                                                                                       gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTg                                                                                                                                                                                   <  1:16614/185‑1 (MQ=21)                                                                                                       gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTtgttgt                                                                                                                 >  1:79598/1‑251 (MQ=255)                                                                                                       gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTtgttgt                                                                                                                 >  1:114133/1‑251 (MQ=255)                                                                                                       gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTtgttgt                                                                                                                 >  1:807378/1‑251 (MQ=255)                                                                                                       gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTtgttgt                                                                                                                 >  1:297376/1‑251 (MQ=255)                                                                                                       gTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATACGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTaaaa                                                                                                                                                  >  1:649152/1‑218 (MQ=255)                                                                                                          tataAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAg                                                                                                                                                                                                                <  1:615852/153‑1 (MQ=17)                                                                                                               ggAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGt                                                                                                           >  1:7322/1‑249 (MQ=255)                                                                                                                gAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGtct                                                                                                         <  1:793161/250‑1 (MQ=255)                                                                                                                gAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGtct                                                                                                         >  1:404344/1‑250 (MQ=255)                                                                                                                gAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGtc                                                                                                          >  1:707668/1‑249 (MQ=255)                                                                                                                      ggAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGc                                                                                                                                                       >  1:728366/1‑198 (MQ=255)                                                                                                                                 aCATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACTCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGcc                                                                                                                                                                                                                       >  1:649431/1‑123 (MQ=9)                                                                                                                                 aCATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGc                                                                                                                                                                                                                        >  1:524838/1‑122 (MQ=11)                                                                                                                                    tGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTGTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGaa                                                                                    <  1:433788/251‑1 (MQ=255)                                                                                                                                      aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAg                                                                                  >  1:156630/1‑251 (MQ=255)                                                                                                                                      aTTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAg                                                                                  >  1:38189/1‑251 (MQ=255)                                                                                                                                              tAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCTCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAg                                                                          <  1:448156/251‑1 (MQ=255)                                                                                                                                              tAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAg                                                                          <  1:189806/251‑1 (MQ=255)                                                                                                                                              tAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAg                                                                          <  1:765615/251‑1 (MQ=255)                                                                                                                                                                atatGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGAc                                                                                                                                >  1:567567/1‑179 (MQ=255)                                                                                                                                                                 tatGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAattc                                                                                                  >  1:642302/1‑208 (MQ=255)                                                                                                                                                                 tatGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAACAGCTTATTg                                                        >  1:738604/1‑250 (MQ=255)                                                                                                                                                                 tatGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGc                                                       <  1:182577/251‑1 (MQ=255)                                                                                                                                                                   tgtgGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAg                                                                          <  1:611023/230‑1 (MQ=255)                                                                                                                                                                           ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCt                                                                                                                                                                                                        <  1:239176/96‑1 (MQ=21)                                                                                                                                                                           ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAattcattc                                                                                              <  1:461475/202‑1 (MQ=255)                                                                                                                                                                           ccTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATg                                             >  1:368567/1‑251 (MQ=255)                                                                                                                                                                            cTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAg                                                               >  1:883993/1‑232 (MQ=255)                                                                                                                                                                                 aGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAg                                                                          <  1:791961/216‑1 (MQ=255)                                                                                                                                                                                         gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTa                                                                                                                                                                         <  1:872458/113‑1 (MQ=18)                                                                                                                                                                                         gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTaaaaaa                                                                                                                                                >  1:163679/1‑138 (MQ=255)                                                                                                                                                                                         gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAg                                                                                  <  1:667338/200‑1 (MQ=255)                                                                                                                                                                                         gTCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGa                               >  1:770265/1‑251 (MQ=255)                                                                                                                                                                                          tCATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCG‑TATGTTTTTTGTGCTAGaa                              <  1:161021/250‑1 (MQ=255)                                                                                                                                                                                               gagTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCg                          >  1:378821/1‑250 (MQ=255)                                                                                                                                                                                               gagTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCg                          >  1:784670/1‑250 (MQ=255)                                                                                                                                                                                                   tATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCAccc                     <  1:604799/251‑1 (MQ=255)                                                                                                                                                                                                   tATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCAccc                     <  1:60690/251‑1 (MQ=255)                                                                                                                                                                                                        cACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTCTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATcc                <  1:442594/251‑1 (MQ=255)                                                                                                                                                                                                        cACGGTTGTTGGTATTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGAGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATcc                <  1:592589/251‑1 (MQ=255)                                                                                                                                                                                                            gttgttGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTc                                                                                                                                                                   >  1:114875/1‑100 (MQ=255)                                                                                                                                                                                                             ttgttgGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTAt           <  1:596947/251‑1 (MQ=255)                                                                                                                                                                                                                 tgGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTATGGGt       <  1:110779/251‑1 (MQ=255)                                                                                                                                                                                                                      ttCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTATGGGTAGGtt  <  1:656965/251‑1 (MQ=255)                                                                                                                                                                                                                      ttCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTATGGGTAGGtt  <  1:637833/251‑1 (MQ=255)                                                                                                                                                                                                                      ttCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTATGGGTAGGtt  <  1:822286/251‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       TGAAAGGCACGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATACAAAGACAGTGGAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGTCATAGAGTTATTTCACGGTTGTTGGTTTTCACATGCCCTTTGAAGGGCATGTGAAGTACTAGGCCCTTATAGGGTAGCCTAAAAACCGCACGTTTTACGTGCGGATTGTTATTTTTCTTGATTCACAGCTAAAAAACATCTGGAAATTCGACCGCACTTTTTGTTGTTTACGTCTCAAATTCATTCTTTTCATGAAAGCGTATAAGTTATTTAAAAGCTTATTGCTTTCGTTATGTTTTTTGTGCTAGAATCCGCACCCTATCCATTATGGGTAGGTT  >  1/705178‑705641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑