| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 698,183 | C→T | A98A (GCG→GCA) | unknown ← | Aspartate‑semialdehyde dehydrogenase (EC 1.2.1.11) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 698,183 | 0 | C | T | 98.7% | 224.2 / ‑5.0 | 79 | A98A (GCG→GCA) | unknown | Aspartate‑semialdehyde dehydrogenase (EC 1.2.1.11) |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (35/43); total (36/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
CACCAATAATTCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCCGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTGATATTCGTAAAATCATTTTCCTGCTGCATACGAT > 1/697943‑698421 | cACCAATAATTCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCAcc < 1:798981/250‑1 (MQ=255)cACCAATAATTCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCAcc > 1:385690/1‑250 (MQ=255) ccAATAATTCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAg > 1:489095/1‑251 (MQ=255) tacttCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAAACTTTTTCAAATAAGCCGCCTAACGATATGAGCATCACGCTGACAGTACGGTTACCGCATACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAAc < 1:422750/248‑1 (MQ=255) taatTCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAAc < 1:247491/251‑1 (MQ=255) atatTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGcc < 1:669092/251‑1 (MQ=255) ttCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGc < 1:851682/251‑1 (MQ=255) ccGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATc < 1:528473/218‑1 (MQ=255) ccGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTaa > 1:516781/1‑251 (MQ=255) acTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGAt > 1:754695/1‑251 (MQ=255) gcTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAAc < 1:741839/214‑1 (MQ=255) gcTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACtt > 1:292002/1‑250 (MQ=255) cAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATcg < 1:374991/251‑1 (MQ=255) cAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATc < 1:56517/250‑1 (MQ=255) cAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATc < 1:298502/250‑1 (MQ=255) gaTCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCtt > 1:77882/1‑251 (MQ=255) aTCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAg < 1:349673/189‑1 (MQ=255) ccATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTgg > 1:317730/1‑250 (MQ=255) ccATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTgg > 1:114375/1‑250 (MQ=255) ttCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAgg > 1:828773/1‑251 (MQ=255) aaaTCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGAt < 1:777413/251‑1 (MQ=255) aTCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATaa < 1:189185/251‑1 (MQ=255) tttCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTcc < 1:716936/251‑1 (MQ=255) cAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGa < 1:445860/200‑1 (MQ=255) aaaCCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGtttttt > 1:709318/1‑251 (MQ=255) gCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCAcc < 1:31485/154‑1 (MQ=255) gCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAAc < 1:289349/161‑1 (MQ=255) gCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTcc > 1:792536/1‑238 (MQ=255) gCCTAACGCTATGAGCATAAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCGCCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAAt < 1:397545/209‑1 (MQ=255) cGCTATGAGCATCAAGCTGACGGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGt < 1:873914/244‑1 (MQ=255) cGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGAt < 1:344296/225‑1 (MQ=255) cGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCaa < 1:124090/251‑1 (MQ=255) gCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGaa > 1:219839/1‑250 (MQ=255) cAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCAt > 1:559141/1‑251 (MQ=255) tGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTc < 1:7597/251‑1 (MQ=255) acagtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAg < 1:480437/224‑1 (MQ=255) acagtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCaa > 1:434809/1‑251 (MQ=255) acagtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCaa > 1:351155/1‑251 (MQ=255) acagtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCaa > 1:804265/1‑251 (MQ=255) gtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAAc > 1:267976/1‑133 (MQ=255) gtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGtt < 1:319015/214‑1 (MQ=255) gtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGc < 1:618395/239‑1 (MQ=255) gtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGt > 1:393380/1‑251 (MQ=255) gtacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGCCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCaatgg > 1:457024/1‑248 (MQ=255) tacagtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAg < 1:279664/248‑1 (MQ=255) gtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTa > 1:340955/1‑126 (MQ=255) gtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCgg > 1:490618/1‑251 (MQ=255) gtTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACG‑CTCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCgg > 1:805093/1‑250 (MQ=255) cTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATc < 1:657863/169‑1 (MQ=255) tACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTAc < 1:109242/251‑1 (MQ=255) aCATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATcgc < 1:683715/169‑1 (MQ=255) gTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCAtt < 1:57721/210‑1 (MQ=255) tttCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGc > 1:701218/1‑204 (MQ=255) tttCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTAccgccg > 1:623615/1‑235 (MQ=255) tttCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCtt > 1:171859/1‑251 (MQ=255) tttCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCtt > 1:252841/1‑251 (MQ=255) aGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTcc < 1:390103/250‑1 (MQ=255) ccTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTg > 1:365824/1‑250 (MQ=255) tCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAAc > 1:265476/1‑88 (MQ=255) cTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGa < 1:604309/151‑1 (MQ=255) cTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTgaga > 1:822686/1‑251 (MQ=255) tGATCACGTGTTGATTCACCGGGTCCAACACGTTAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGaa < 1:361426/251‑1 (MQ=255) gATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGa > 1:221284/1‑149 (MQ=255) gATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGttt > 1:690001/1‑165 (MQ=255) ttGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAaag < 1:709234/250‑1 (MQ=255) tGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAg < 1:336516/241‑1 (MQ=255) ggTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGa < 1:898007/129‑1 (MQ=255) ggTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTg > 1:499778/1‑251 (MQ=255) gTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAg > 1:590692/1‑212 (MQ=255) gTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAg < 1:343639/230‑1 (MQ=255) gTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAg > 1:827323/1‑230 (MQ=255) cAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTGatat < 1:565010/251‑1 (MQ=255) cGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGtt < 1:568474/135‑1 (MQ=255) cGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTcc < 1:417114/211‑1 (MQ=255) cttcCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAaag > 1:421826/1‑215 (MQ=255) tcCATCCGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAATGGTAAAGAAGATCGGATTGATATTCGTAAAATCATTTTc < 1:897577/245‑1 (MQ=255) tcCATACGCAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTGATATTCGTAAAATCATTTTCctg > 1:766969/1‑248 (MQ=255) cAATGCGGACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTGATATTCGTAAAATCATTTTCCTGCTGCATACGAt < 1:188916/251‑1 (MQ=255) gACGCTGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTGata < 1:140180/213‑1 (MQ=255) | CACCAATAATTCGCGCATATTTTTCGCACCGGCACCACTGGCGGCTTGGTAAGTGGCAACGGAGATCCATTCCACTAAATCTTTTTCAAATAAACCGCCTAACGCTATGAGCATCAAGCTGACAGTACAGTTACCGCCTACATAGGTTTTCACGCCGTTTTTCAAGCCTTCGCTGATCACGTGTTGATTCACCGGGTCCAACACGATAATCGCGTCTTCTTCCATACGCAATGCGGACGCCGCATCCACCCAGTAACCGCTCCAGCCGGTGGCTTTTAATTTCGGATAAACTTCGTTGGTGTAATCGCCGCCTTGGCAGGTGACGATAATGTCCAGTTTTTTCAGTTCTTCAATGTCGAACGCATTTTTCAAGGTGCCGGCGTCTTTACCGCCGAATGTCGGTGCAGCTTGTCCTGCTTGAGAAGTGGTAAAGAAGATCGGATTGATATTCGTAAAATCATTTTCCTGCTGCATACGAT > 1/697943‑698421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |