Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	663,921	G→A	E338E (GAG→GAA)	unknown →	Predicted membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	663,921	0	G	A	100.0%	211.2 / NA	73	E338E (GAG→GAA)	unknown	Predicted membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/33);  total (40/33)

ACGCTTTATCGGCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAGTTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTCACCGCTTC  >  1/663678‑664163                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   aCGCTTTATCGGCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAacac                                                                                                                                                                                                                                             <  1:333537/251‑1 (MQ=255)  cGCTTTATCGGCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAacac                                                                                                                                                                                                                                             <  1:670268/250‑1 (MQ=255)         tCGGCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtg                                                                                                                                                                                                                                     <  1:196429/251‑1 (MQ=255)         tCGGCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtg                                                                                                                                                                                                                                     <  1:234038/251‑1 (MQ=255)            gCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtggtg                                                                                                                                                                                                                                  >  1:644472/1‑251 (MQ=255)                   gACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtgg                                                                                                                                                                                                                                    <  1:872874/242‑1 (MQ=255)                   gACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGtt                                                                                                                                                                                                                            <  1:54988/250‑1 (MQ=255)                     cTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAacac                                                                                                                                                                                                                                             >  1:90979/1‑231 (MQ=255)                     cTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGt                                                                                                                                                                                                                         >  1:428338/1‑251 (MQ=255)                       aTCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:891374/251‑1 (MQ=255)                       aTCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:479095/251‑1 (MQ=255)                       aTCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:562618/251‑1 (MQ=255)                       aTCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:735303/251‑1 (MQ=255)                       aTCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:276068/251‑1 (MQ=255)                            tATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTcc                                                                                                                                                                                                                  <  1:507726/251‑1 (MQ=255)                            tATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTc                                                                                                                                                                                                                   <  1:45720/250‑1 (MQ=255)                                     tATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtg                                                                                                                                                                                                                                     <  1:677141/223‑1 (MQ=255)                                          ggACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTAt                                                                                                                                                                                                     >  1:201711/1‑250 (MQ=255)                                             ccTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGt                                                                                                                                                                                                 >  1:196155/1‑251 (MQ=255)                                             ccTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGt                                                                                                                                                                                                 >  1:884642/1‑251 (MQ=255)                                              ggtccagatagccgcaataCCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACa                                                                                                                                                                                                                      >  1:240295/19‑229 (MQ=255)                                               ttATTGATTATACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATAAGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGGGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGttt                                                                                                                                                                                               >  1:158424/1‑251 (MQ=255)                                                tATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTcc                                                                                                                                                                                                                  <  1:877160/231‑1 (MQ=255)                                                     aTTCTTCTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTcc                                                                                                                                                                                                                  <  1:617568/226‑1 (MQ=255)                                                     aTTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCAt                                                                                                                                                                                         <  1:518225/251‑1 (MQ=255)                                                        ctactaAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtggtggtgg                                                                                                                                                                                                                              >  1:76932/1‑211 (MQ=255)                                                         tactaAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTcc                                                                                                                                                                                                                  >  1:529026/1‑222 (MQ=255)                                                           ctaAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGc                                                                                                                                                                                      >  1:563021/1‑248 (MQ=255)                                                                 ccGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGt                                                                                                                                                                              >  1:211545/1‑250 (MQ=255)                                                                       gTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTa                                                                                                                                                                       >  1:296503/1‑251 (MQ=255)                                                                        tACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtgg                                                                                                                                                                                                                                    <  1:95113/189‑1 (MQ=255)                                                                         aCAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtggt                                                                                                                                                                                                                                   >  1:217651/1‑189 (MQ=255)                                                                            aGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATgg                                                                                                                                                                  >  1:267394/1‑251 (MQ=255)                                                                                        ttCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTc                                                                                                                                                                                                                   <  1:41111/190‑1 (MQ=255)                                                                                        ttCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAg                                                                                                                                                      >  1:12738/1‑251 (MQ=255)                                                                                                    gTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:432186/174‑1 (MQ=255)                                                                                                    gTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCa                                                                                                                                          >  1:179233/1‑251 (MQ=255)                                                                                                             aCATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATtggt                                                                                                                                                                                                                                   <  1:759172/153‑1 (MQ=255)                                                                                                                       gAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCAt                                                                                                                                                                                         >  1:699974/1‑185 (MQ=255)                                                                                                                        aaTACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCggg                                                                                                                      >  1:808991/1‑251 (MQ=255)                                                                                                                                cATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTACATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAg                                                                                                                                                      <  1:436610/211‑1 (MQ=255)                                                                                                                                  tCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCg                                                                                                            >  1:709593/1‑251 (MQ=255)                                                                                                                                    gTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTc                                                                                                              >  1:195809/1‑247 (MQ=255)                                                                                                                                    gTATTGATATAGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGcc                                                                                                                                       >  1:66310/1‑222 (MQ=255)                                                                                                                                      attgatattgaCCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTc                                                                                                              >  1:326560/1‑245 (MQ=255)                                                                                                                                         gatattgaCCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATcg                                                                                                      >  1:35021/1‑250 (MQ=255)                                                                                                                                         gatattgaCCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATcg                                                                                                      >  1:301929/1‑250 (MQ=255)                                                                                                                                          atattgaCCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTa                                                                                                                                                                                                                                                 <  1:123806/110‑1 (MQ=255)                                                                                                                                          atattgaCCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATcgcg                                                                                                    >  1:265304/1‑251 (MQ=255)                                                                                                                                                aCCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCATCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTAttt                                                                                                               >  1:426085/1‑234 (MQ=255)                                                                                                                                                       gCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGcc                                                                                                                                       >  1:391051/1‑203 (MQ=255)                                                                                                                                                        ccGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTAc                                                                                                                                                                                                                       <  1:574374/122‑1 (MQ=255)                                                                                                                                                        ccGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGtt                                                                                      >  1:747084/1‑251 (MQ=255)                                                                                                                                                              aCCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCttctgtctctataca                                                                                >  1:834400/1‑238 (MQ=255)                                                                                                                                                                   gATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCg                                                                           >  1:865477/1‑251 (MQ=255)                                                                                                                                                                     tttATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACTGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGgtg                                                                                        <  1:435955/236‑1 (MQ=255)                                                                                                                                                                         tatTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATg                                                                     >  1:528561/1‑251 (MQ=255)                                                                                                                                                                                       cTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTAttt                                                       >  1:822086/1‑251 (MQ=255)                                                                                                                                                                                         gTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGa                                                     >  1:761527/1‑251 (MQ=255)                                                                                                                                                                                            aTCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAac                                                  <  1:579067/251‑1 (MQ=255)                                                                                                                                                                                             tCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAac                                                  >  1:393164/1‑250 (MQ=255)                                                                                                                                                                                                 gATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCg                                             >  1:473961/1‑251 (MQ=255)                                                                                                                                                                                                     gtgATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATgg                                         >  1:215345/1‑251 (MQ=255)                                                                                                                                                                                                                      cTTTATCGCTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCg                        >  1:638921/1‑251 (MQ=255)                                                                                                                                                                                                                              cTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGAcc                <  1:682004/251‑1 (MQ=255)                                                                                                                                                                                                                              cTGCCGCACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGAcc                <  1:824811/251‑1 (MQ=255)                                                                                                                                                                                                                                    cACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTc          <  1:706413/251‑1 (MQ=255)                                                                                                                                                                                                                                    cACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTc          <  1:559563/251‑1 (MQ=255)                                                                                                                                                                                                                                    cACCGATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTc          <  1:117671/251‑1 (MQ=255)                                                                                                                                                                                                                                        gATTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTCACCg      >  1:509520/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTCACCg      <  1:286/250‑1 (MQ=255)                                                                                                                                                                                                                                         aTTTCACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTCACCg      <  1:583124/250‑1 (MQ=255)                                                                                                                                                                                                                                             cACCTGAATTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTCACCGCTTc  >  1:868166/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ACGCTTTATCGGCGTAGGGACTATCGGTATTGCGGCTATCTGGACCTTATTGATTCTACTAAAACCGATGGTACAAGGTATGGCGCATTCTTTCCGTATGTTGAAGGGACATCAGGCAGAATACGAACATCGTATTGATATTGACCTATCGCCGAAAACCATGATTTATATTTTGATCGCGACTGTTATCTTGATTGTGATTTCTTTACATCACTTTATCGCTGCCGCACCGATTTCACCTGAGTTAACACTCTTATTGGTGGTGGTTTGTACATTCCTTGCAGTTTTTATCGGTTTTTTCATAGCGGCAGCGTCCGGTTACATGGCCGGGCTTGTAGGGGCCTCTTCCAGCCCGATTTCCGGTATCGGGATTATTTCCGTCATCGCGATTTCTTTGGTGTTGGTTTCTATCGGTAATGCATCCGGTTTATTTGAAACCACCGATGGACAGAAATTTTTGACCGCACTGACCTTGTTCACCGCTTC  >  1/663678‑664163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑