Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	643,539	T→C	N89S (AAT→AGT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	643,539	0	T	C	100.0%	93.5 / NA	32	N89S (AAT→AGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/10);  total (22/10)

GCTAAATATATTTCTGTTAGAGCTGCTTTAGACTAATCTAATTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATGTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATATTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAACTTATCCTTTATCTCTT  >  1/643308‑643782                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    gCTAAATATATTTCTGTTAGAGCTGCTTTAGACTAATCTAATTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATagag                                                                                                                                                                                                                                    >  1:389903/1‑249 (MQ=255)          atTTCTGTTAGAGCTGCTTTAGACTAATCTAATTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTctacta                                                                                                                                                                                                                                             <  1:832168/231‑1 (MQ=255)                         gctTTAGACTAATCTAATTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCaaa                                                                                                                                                                                                          >  1:895954/1‑251 (MQ=255)                         gctTTAGACTAATCTAATTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCaaa                                                                                                                                                                                                          >  1:450807/1‑251 (MQ=255)                                   aatctaatTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATa                                                                                                                                                                                                 >  1:726123/1‑250 (MQ=255)                                                  cAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGc                                                                                                                                                                                 <  1:522455/251‑1 (MQ=255)                                                        cttACGAAAGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACaa                                                                                                                                                                            >  1:106563/1‑250 (MQ=255)                                                              aaaGAATTCGAGTAAATTCTCGTAATTTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCAtt                                                                                                                                                                      <  1:501545/250‑1 (MQ=255)                                                                                                          gggAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTAcc                                                                                                                                     <  1:734356/239‑1 (MQ=255)                                                                                                             aaTGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGc                                                                                                                       <  1:417137/250‑1 (MQ=255)                                                                                                                 ggTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATc                                                                                                                   >  1:268594/1‑250 (MQ=255)                                                                                                                                     ttATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTc                                                                                                                                                                         >  1:147419/1‑176 (MQ=255)                                                                                                                                                     cttcttTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGc                                                                                                                       >  1:133040/1‑210 (MQ=255)                                                                                                                                                     cttcttTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGtt                                                                                >  1:676218/1‑249 (MQ=255)                                                                                                                                                            aGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGc                                                                                                                                                                                                        >  1:524964/1‑122 (MQ=255)                                                                                                                                                                                   tttttAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGAtttt                                                  >  1:869461/1‑249 (MQ=255)                                                                                                                                                                                            ggTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAAc                                                                                                                                                                           <  1:771852/119‑1 (MQ=255)                                                                                                                                                                                            ggTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATtac                                                                                                                                                                    >  1:165786/1‑126 (MQ=255)                                                                                                                                                                                            ggTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGaaa                                                                                                             >  1:160202/1‑181 (MQ=255)                                                                                                                                                                                            ggTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATc                                                                         >  1:149695/1‑217 (MQ=255)                                                                                                                                                                                                 atatTGATCACATTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATaa                                  >  1:859152/1‑251 (MQ=255)                                                                                                                                                                                                           caTTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGaaa                                                                                                             >  1:790315/1‑166 (MQ=255)                                                                                                                                                                                                           caTTTAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAAt                        >  1:193597/1‑251 (MQ=255)                                                                                                                                                                                                             tttAAAGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTc                                                                               <  1:28997/194‑1 (MQ=255)                                                                                                                                                                                                                 aaGTATCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAAc                  <  1:454068/251‑1 (MQ=255)                                                                                                                                                                                                                     aTCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATaa                                                                                                                                                                                                                              <  1:870895/43‑1 (MQ=255)                                                                                                                                                                                                                     aTCTTCAGCCTCTTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCa                                                                                                   <  1:270614/166‑1 (MQ=255)                                                                                                                                                                                                                                ctTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAACTTATcc            >  1:358071/1‑242 (MQ=255)                                                                                                                                                                                                                                ctTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAACTTATCCTTTAtct     >  1:571170/1‑249 (MQ=255)                                                                                                                                                                                                                                ctTTAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAACTTATCCTTTAtct     >  1:810695/1‑249 (MQ=255)                                                                                                                                                                                                                                  ttAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCGTTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAACTTATCCTTTATCtctt  >  1:322744/1‑250 (MQ=255)                                                                                                                                                                                                                                  ttAATACTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATc                                                                                                                            >  1:341836/1‑128 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    GCTAAATATATTTCTGTTAGAGCTGCTTTAGACTAATCTAATTTAAACCCAACTTCTTACGAAAGAATTCGAGTAAATTCTCGTAATGTTCGACTATTCCCATCAGGGAATGGGTGAATGTAATCTAGCTTTTTATAAATATCTACAACTTCTTTAGCAAATTCATCTTTATTAAAAGTTTTTAATTGGTTTATATTGATCACATTTAAAGTATCTTCAGCCTCTTTAATATTTCTACTATCCATAGAGCTATAAAAAACGGTTATATTCCCAAAGCCCTGATAATGGCGTACCTTATGCCACAACTCATTACTACCTATTTCAACTTCAGATCTAAATTTACCAGCTACATCAGGGCTATCCTGAAAAATATAACCATTTAATCTTTTCAGATGTTCTAAATCAAATTTCCCTTTTACAGGATTTTCTCTCAATTCTGATAAACGAATAAATGACAACTTATCCTTTATCTCTT  >  1/643308‑643782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑