Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	642,657	G→A	G127G (GGG→GGA)	unknown →	cell filamentation‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	642,657	0	G	A	100.0%	270.9 / NA	95	G127G (GGG→GGA)	unknown	cell filamentation‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/44);  total (51/44)

ATTCAATTAACGGTAAATTTGATTTAAAGCACCTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGGGAAATTGACGAATAAATACAACGTATCAAGAGCCACAATAAGCAGATATAAAAAACGAGCAAAATAACTACCGCTTCCCTGCGGTTCTTGCTGAATTCTCAAAGAGTAAAAACAGACTACGCTCTTTTTTTGCTCTTTAGACTATTTTTTATAGAGAAAAACCTTTAGATTTTTGAGCTTGTTGTTCCTGAGATTTAGGTTTAATTTTA  >  1/642413‑642866                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                  aTTCAATTAACGGTAAATTTGATTTAAAGCACCTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATT                                                                                                                                                                                                             >  1:637882‑M1/1‑251 (MQ=255)                      aTTTAAAGCACCTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttag                                                                                                                                                                                         <  1:775159‑M1/250‑21 (MQ=255)                       tttAAAGCACCTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCTCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagag                                                                                                                                                                                       <  1:556606‑M1/251‑23 (MQ=255)                          aaaGCACCTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTTAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagagg                                                                                                                                                                                    >  1:665364‑M1/1‑226 (MQ=255)                                ccTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacg                                                                                                                                                                              >  1:588371‑M1/1‑220 (MQ=255)                                ccTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacg                                                                                                                                                                              >  1:603396‑M1/1‑220 (MQ=255)                                ccTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagag                                                                                                                                                                                       >  1:871825‑M1/1‑220 (MQ=255)                                ccTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttag                                                                                                                                                                                         >  1:325540‑M1/1‑220 (MQ=255)                                ccTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacaccc                                                                                                                                                                                               >  1:550630‑M1/1‑220 (MQ=255)                                    cAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaag                                                                                                                                                                          <  1:153515‑M1/251‑36 (MQ=255)                                                     attattCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacc                                                                                                                                                                                                >  1:55218‑M1/1‑199 (MQ=255)                                                      ttattCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttat                                                                                                                                                        <  1:244355‑M1/251‑54 (MQ=255)                                                        attCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatag                                                                                                                                                      <  1:387051‑M1/251‑56 (MQ=255)                                                        attCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatag                                                                                                                                                      >  1:351064‑M1/1‑196 (MQ=255)                                                                atatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacactcttttagagaggggaacggaagaacacaacgaatctttatagctcaatta                                                                                                                                              >  1:508444‑M1/1‑188 (MQ=255)                                                                atatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaatt                                                                                                                                               <  1:270692‑M1/250‑63 (MQ=255)                                                                atatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagct                                                                                                                                                    >  1:150094‑M1/1‑188 (MQ=255)                                                                atatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacaccctttt                                                                                                                                                                                           >  1:677005‑M1/1‑188 (MQ=255)                                                                 tatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattag                                                                                                                                             >  1:297670‑M1/1‑187 (MQ=255)                                                                 tatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacac                                                                                                                                                                     <  1:237626‑M1/227‑41 (MQ=255)                                                                 tatTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagag                                                                                                                                                                                       <  1:837039‑M1/209‑23 (MQ=255)                                                                 tatTTATCACTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgc                                                                                                                                                                                                        <  1:483102‑M1/192‑6 (MQ=255)                                                                  atTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattag                                                                                                                                             >  1:551685‑M1/1‑186 (MQ=255)                                                                                                ggATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacaccctttagag                                                                                                                                                                                        <  1:559535‑M1/177‑22 (MQ=255)                                                                                                       tACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATT                                                                                                                                                                                                             >  1:161719‑M1/1‑149 (MQ=255)                                                                                                        aCAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaa                                                                                                      >  1:358971‑M1/1‑148 (MQ=255)                                                                                                        aCAAAAAGGTGAACAAGATTTCACTACGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatc                                                                                                                                                             <  1:441283‑M1/196‑49 (MQ=255)                                                                                                         cAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaa                                                                                                      >  1:643818‑M1/1‑147 (MQ=255)                                                                                                             aaGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaa                                                                                                 >  1:375381‑M1/1‑143 (MQ=255)                                                                                                             aaGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaat                                                                                                                        >  1:178211‑M1/1‑143 (MQ=255)                                                                                                             aaGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgc                                                                                                                                                                                                        >  1:169966‑M1/1‑143 (MQ=255)                                                                                                               ggTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaaga                                                                                               >  1:487655‑M1/1‑141 (MQ=255)                                                                                                               ggTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaac                                                                                                                                                                       >  1:235693‑M1/1‑141 (MQ=255)                                                                                                                  gAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttat                                                                                                                                                        <  1:738034‑M1/191‑54 (MQ=255)                                                                                                                               cTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTgacgctatacacccttttagagaggggaacggaagaacacaacggatctttatag                                                                                                                                                      >  1:447524‑M1/1‑125 (MQ=255)                                                                                                                               cTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaag                                                                                             <  1:868017‑M1/237‑113 (MQ=255)                                                                                                                                 aTGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgctt                                                                              >  1:350658‑M1/1‑123 (MQ=255)                                                                                                                                 aTGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgctt                                                                              >  1:700439‑M1/1‑123 (MQ=255)                                                                                                                                     gCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaatt                                                                         >  1:659848‑M1/1‑119 (MQ=255)                                                                                                                                        aTCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaa                                                                      >  1:54677‑M1/1‑116 (MQ=255)                                                                                                                                        aTCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaa                                                                      >  1:708241‑M1/1‑116 (MQ=255)                                                                                                                                        aTCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagc                                                                                                                                                     >  1:900463‑M1/1‑116 (MQ=255)                                                                                                                                        aTCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttag                                                                                                                                                                                         <  1:877803‑M1/136‑21 (MQ=255)                                                                                                                                        aTCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacc                                                                                                                                                                                                >  1:88098‑M1/1‑116 (MQ=255)                                                                                                                                         tCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaag                                                                     >  1:817672‑M1/1‑115 (MQ=255)                                                                                                                                         tCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacgga                                                                                                                                                                            <  1:167131‑M1/148‑34 (MQ=255)                                                                                                                                           gTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagtt                                                                   >  1:559296‑M1/1‑113 (MQ=255)                                                                                                                                           gTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagtt                                                                   >  1:117582‑M1/1‑113 (MQ=255)                                                                                                                                           gTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagtt                                                                   >  1:486305‑M1/1‑113 (MQ=255)                                                                                                                                           gTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttag                                                                                                                                                                                         <  1:267768‑M1/133‑21 (MQ=255)                                                                                                                                           gTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgc                                                                                                                                                                                                        <  1:653362‑M1/118‑6 (MQ=255)                                                                                                                                              ttattCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcat                                                                >  1:615107‑M1/1‑110 (MQ=255)                                                                                                                                                attCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatag                                                              <  1:235310‑M1/251‑144 (MQ=255)                                                                                                                                                        aTCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagc                                                                                                                                                     <  1:783770‑M1/156‑57 (MQ=255)                                                                                                                                                        aTCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagag                                                                                                                                                                                       <  1:260439‑M1/122‑23 (MQ=255)                                                                                                                                                          cTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattat                                                    >  1:280090‑M1/1‑98 (MQ=255)                                                                                                                                                          cTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattat                                                    >  1:658230‑M1/1‑98 (MQ=255)                                                                                                                                                          cTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattat                                                    >  1:681050‑M1/1‑98 (MQ=255)                                                                                                                                                          cTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacc                                                                                                                                                                                                <  1:551753‑M1/111‑14 (MQ=255)                                                                                                                                                             aaaGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattaaaaa                                                 <  1:858186‑M1/251‑157 (MQ=255)                                                                                                                                                             aaaGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagtttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaag                                                 <  1:798831‑M1/251‑157 (MQ=255)                                                                                                                                                              aaGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTAGATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaag                                                <  1:446843‑M1/251‑158 (MQ=255)                                                                                                                                                                   tGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagtt                                                                                                                  <  1:377210‑M1/180‑92 (MQ=255)                                                                                                                                                                    gTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttg                                          >  1:552014‑M1/1‑88 (MQ=255)                                                                                                                                                                    gTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatg                                                           >  1:399942‑M1/1‑88 (MQ=255)                                                                                                                                                                     tttAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttg                                          <  1:538899‑M1/250‑164 (MQ=255)                                                                                                                                                                       tAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgaga                                       <  1:361506‑M1/251‑167 (MQ=255)                                                                                                                                                                       tAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgaga                                       <  1:349591‑M1/251‑167 (MQ=255)                                                                                                                                                                         aaGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaat                                     <  1:534268‑M1/251‑169 (MQ=255)                                                                                                                                                                              cTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattat                                >  1:501476‑M1/1‑78 (MQ=255)                                                                                                                                                                                          cAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggc                     <  1:633427‑M1/250‑185 (MQ=255)                                                                                                                                                                                              ttttGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaact                <  1:58358‑M1/251‑190 (MQ=255)                                                                                                                                                                                               tttGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactc               <  1:825118‑M1/251‑191 (MQ=255)                                                                                                                                                                                               tttGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactc               <  1:2440‑M1/251‑191 (MQ=255)                                                                                                                                                                                               tttGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactc               <  1:796764‑M1/251‑191 (MQ=255)                                                                                                                                                                                                ttGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcc              <  1:174474‑M1/251‑192 (MQ=255)                                                                                                                                                                                                      aaaaCAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctatc        <  1:409195‑M1/251‑198 (MQ=255)                                                                                                                                                                                                      aaaaCAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctat         <  1:282310‑M1/250‑197 (MQ=255)                                                                                                                                                                                                       aaaCAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctat         >  1:310604‑M1/1‑53 (MQ=255)                                                                                                                                                                                                       aaaCAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctct           >  1:857203‑M1/1‑53 (MQ=255)                                                                                                                                                                                                        aaCAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgag                                        >  1:63241‑M1/1‑52 (MQ=255)                                                                                                                                                                                                            gagTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaag                                                <  1:507845‑M1/205‑158 (MQ=255)                                                                                                                                                                                                                cTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctatctctaaa  >  1:546241‑M1/1‑44 (MQ=255)                                                                                                                                                                                                                                tAGATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagg                                               <  1:24789‑M1/186‑159 (MQ=255)                                                                                                                                                                                                                                  gATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctat         >  1:103696‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                  gATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaag                                                >  1:627358‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                  gATTAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatg                                                                                        <  1:753775‑M1/143‑118 (MQ=255)                                                                                                                                                                                                                                    ttAGCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaag                                                                                   <  1:213247‑M1/146‑123 (MQ=255)                                                                                                                                                                                                                                       gCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctatc        >  1:54420‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                       gCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaag                        <  1:878199‑M1/202‑182 (MQ=255)                                                                                                                                                                                                                                       gCTTACTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggc                                             >  1:708295‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                            cTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaag                                                <  1:27692‑M1/173‑158 (MQ=255)                                                                                                                                                                                                                                            cTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgaca                                                                                     <  1:5911‑M1/136‑121 (MQ=255)                                                                                                                                                                                                                                            cTATATGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaac                                                                                                        >  1:151860‑M1/1‑16 (MQ=255)                                                                                                                                                                                                                                              atatGGGAGAAATTaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagag                                                                                                                                     >  1:442895‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                  ATTCAATTAACGGTAAATTTGATTTAAAGCACCTTCAAGCTATCCATAAAAAATTATTCGAAGATATTTATCCCTTTGCTGGAAAAATAAGAGATGGATATTTACAAAAAGGTGAACAAGATTTCACTATGGGCTATCGTATTATTCCGCAATCTGAAAGACTGTTTAAAGAACTCAAAAATGAGCAATTTTTGAAGAAAACAGAGTCTGATAAAATTGCGGGTAGATTAGCTTACTATATGGGGGAAATTGACGAATAAATACAACGTATCAAGAGCCACAATAAGCAGATATAAAAAACGAGCAAAATAACTACCGCTTCCCTGCGGTTCTTGCTGAATTCTCAAAGAGTAAAAACAGACTACGCTCTTTTTTTGCTCTTTAGACTATTTTTTATAGAGAAAAACCTTTAGATTTTTGAGCTTGTTGTTCCTGAGATTTAGGTTTAATTTTA  >  1/642413‑642866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑