Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	593,516	A→G	intergenic (+387/+30)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	593,516	0	A	G	100.0%	134.0 / NA	45	intergenic (+387/+30)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

AAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTTTTGGTCGGCACGAATTAACTCGCGTAAAGAACCAAAATGTTGCAATACATTTTTCGATAACTGCATCACCGGGCAGTCCTTAATTCCCGTACGCAAAAAGATCGCCAATAGTTCATCGTCCGTCAAAGCGGCAGCACCGTATTTTAATAGCTTTTCCCTTGGCATTAATGCTTCATTCATTTGCCGAACCTCTCTAAA  >  1/593362‑593761                                                                                                                                                            |                                                                                                                                                                                                                                                      aaaCGGCGGGTTAATACTCCGCCGTTTTTCTTTTCATGTACATGATGATTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaatgt                                                                                                                                                                                                        <  1:82718/203‑12 (MQ=255)                                           tgatgaTTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAacac                                                                                                                                                                                                                    >  1:770647/1‑149 (MQ=255)                                                     gctttAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaa                                                                                                                                                                                                           >  1:450714/4‑140 (MQ=255)                                                                                                       gcgctatttggcgttaaaccacataagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggtta                                                  >  1:842229‑M1/16‑90 (MQ=255)                                                                                                          ctatttggcgttaaaccacataagtttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                                               >  1:419408‑M1/13‑87 (MQ=255)                                                                                                          ctatttggcgttaaaccacataagtttAGAAATGTTGTTTCTAGGTTTATGTGGTCATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                                               >  1:174329‑M1/13‑87 (MQ=255)                                                                                                            atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                                               >  1:111497‑M1/11‑85 (MQ=255)                                                                                                            atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                                               >  1:235911‑M1/11‑85 (MQ=255)                                                                                                            atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgc                                                                                                   >  1:783122‑M1/11‑85 (MQ=255)                                                                                                               tggcgttaaaccacataAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc                                          <  1:599060‑M1/244‑170 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc                                         >  1:88256‑M1/6‑80 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc                                         >  1:57300‑M1/6‑80 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc                                         >  1:880404‑M1/6‑80 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc                                         >  1:268599‑M1/6‑80 (MQ=255)                                                                                                                         ccacataagttTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgggaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaaca                                >  1:450349‑M1/5‑72 (MQ=255)                                                                                                                            cataaGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacatta                             <  1:346124‑M1/250‑183 (MQ=255)                                                                                                                               aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct                          <  1:743567‑M1/248‑186 (MQ=255)                                                                                                                               aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct                          <  1:410578‑M1/248‑186 (MQ=255)                                                                                                                               aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct                          <  1:849248‑M1/248‑186 (MQ=255)                                                                                                                               aagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatct                          <  1:643011‑M1/248‑186 (MQ=255)                                                                                                                                 gtttaGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcg                        >  1:189643‑M1/2‑64 (MQ=255)                                                                                                                                 gtttaGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaat                                                                                                             >  1:401336‑M1/2‑64 (MQ=255)                                                                                                                                  tttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctc                         <  1:274764‑M1/249‑187 (MQ=255)                                                                                                                                  tttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctc                         <  1:42191‑M1/249‑187 (MQ=255)                                                                                                                                    tagaaatgttgtttctaggtttatgTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcgggg                     <  1:708790‑M1/240‑191 (MQ=255)                                                                                                                                    tagaaatgttgtttctaggtttatgTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcgggg                     >  1:838498‑M1/12‑61 (MQ=255)                                                                                                                                          tgttgtttctaggTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagt                                                                                                                                                                 <  1:791297‑M1/100‑51 (MQ=255)                                                                                                                                           gttgtttctagGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttat              >  1:135142‑M1/5‑54 (MQ=255)                                                                                                                                              gtttcTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggg           >  1:501880‑M1/2‑51 (MQ=255)                                                                                                                                              gtttcTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatgga           >  1:607724‑M1/2‑51 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:844343‑M1/251‑202 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:674489‑M1/251‑202 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:6807‑M1/251‑202 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:248526‑M1/251‑202 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:39907‑M1/251‑202 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtc                                                                           >  1:178814‑M1/1‑50 (MQ=255)                                                                                                                                               tttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgtagatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:179977‑M1/251‑202 (MQ=255)                                                                                                                                                ttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttttgggaa         >  1:303294‑M1/1‑49 (MQ=255)                                                                                                                                                ttCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccgg                                                                                                                    >  1:209444‑M1/1‑49 (MQ=255)                                                                                                                                                    aggtttatgTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatata      >  1:514024‑M1/4‑45 (MQ=255)                                                                                                                                                    aggtttatgTGGTTATTTTTCTAAAATCTCGCGGTAGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatata      >  1:148219‑M1/4‑45 (MQ=255)                                                                                                                                                     ggtttatGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatataat    >  1:774391‑M1/3‑44 (MQ=255)                                                                                                                                                     ggtttatGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:481812‑M1/243‑202 (MQ=255)                                                                                                                                                     ggtttatGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaa          <  1:520038‑M1/243‑202 (MQ=255)                                                                                                                                                      gtttaTGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagtcactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatatac     <  1:428715‑M1/248‑207 (MQ=255)                                                                                                                                                      gtttaTGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatatact    <  1:504978‑M1/249‑208 (MQ=255)                                                                                                                                                      gtttaTGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatatac     <  1:692474‑M1/248‑207 (MQ=255)                                                                                                                                                      gtttaTGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatggaatatac     <  1:133077‑M1/248‑207 (MQ=255)                                                                                                                                                       tttATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacattatctcggggcggttatgggatatactaa  >  1:182828‑M1/1‑42 (MQ=255)                                                                                                                                                            |                                                                                                                                                                                                                                                      AAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTTTTGGTCGGCACGAATTAACTCGCGTAAAGAACCAAAATGTTGCAATACATTTTTCGATAACTGCATCACCGGGCAGTCCTTAATTCCCGTACGCAAAAAGATCGCCAATAGTTCATCGTCCGTCAAAGCGGCAGCACCGTATTTTAATAGCTTTTCCCTTGGCATTAATGCTTCATTCATTTGCCGAACCTCTCTAAA  >  1/593362‑593761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑