Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	593,489	A→G	intergenic (+360/+57)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	593,489	0	A	G	100.0%	21.1 / NA	9	intergenic (+360/+57)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/3);  total (6/3)

AAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTTTTGGTCGGCACGAATTAACTCGCGTAAAGAACCAAAATGTTGCAATACATTTTTCGATAACTGCATCACCGGGCAGTCCTTAATTCCCGTACGCAAAAAGATCGCCAATAGTTCATCGTCCGTCAAAGCGGCAGCACCGTATTTTAATAGCTTTTCCCTTGGCATTAATGC  >  1/593362‑593734                                                                                                                                 |                                                                                                                                                                                                                                                      aaaCGGCGGGTTAATACTCCGCCGTTTTTCTTTTCATGTACATGATGATTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaatgt                                                                                                                                                                             <  1:82718/203‑12 (MQ=255)                                           tgatgaTTCTGCTTTAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAacac                                                                                                                                                                                         >  1:770647/1‑149 (MQ=255)                                                     gctttAAATTTGAGGGTAATTAGTCGCTGATGATGTGTAAAATTGACGTAGCGCTATTTGGCGTTAAACCACATAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACgtccggtaa                                                                                                                                                                                >  1:450714/4‑140 (MQ=255)                                                                                                       gcgctatttggcgttaaaccacataagtttagaAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggtta                       >  1:842229‑M1/16‑90 (MQ=255)                                                                                                          ctatttggcgttaaaccacataagtttAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                    >  1:419408‑M1/13‑87 (MQ=255)                                                                                                          ctatttggcgttaaaccacataagtttAGAAATGTTGTTTCTAGGTTTATGTGGTCATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                    >  1:174329‑M1/13‑87 (MQ=255)                                                                                                            atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                    >  1:111497‑M1/11‑85 (MQ=255)                                                                                                            atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatca                    >  1:235911‑M1/11‑85 (MQ=255)                                                                                                            atttggcgttaaaccacataagtTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgc                                                                        >  1:783122‑M1/11‑85 (MQ=255)                                                                                                               tggcgttaaaccacataAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc               <  1:599060‑M1/244‑170 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc              >  1:268599‑M1/6‑80 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc              >  1:57300‑M1/6‑80 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc              >  1:880404‑M1/6‑80 (MQ=255)                                                                                                                 gcgttaaaccacaTAAGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttc              >  1:88256‑M1/6‑80 (MQ=255)                                                                                                                         ccacataagttTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgggaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaaca     >  1:450349‑M1/5‑72 (MQ=255)                                                                                                                            cataaGTTTAGAAATGTTGTTTCTAGGTTTATGTGGTTATTTTTCTAAAATCTCGCGGTCGGCAACACGtccggtaatgttgatttatgcaaaggttacataaagccactggcaagagttattcctgagcaagaagagacggctagtcagggagagtataccggtgagaatcgtgttgcgccgaacccgatgaaatctcgtagtcaatcttggtttttccgcccgggttatcacttttccgagcaacatta  <  1:346124‑M1/250‑183 (MQ=255)                                                                                                                                 |                                                                                                                                                                                                                                                      AAACGGCGGGGTAATAC‑CCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAAATTTAGAAAATTTATTTCTAGTTTTATATGGTTATTTTTCTAAAATCTCGCGGTCGGCTACACGCAAAACTGCTTTTGGTCGGCACGAATTAACTCGCGTAAAGAACCAAAATGTTGCAATACATTTTTCGATAACTGCATCACCGGGCAGTCCTTAATTCCCGTACGCAAAAAGATCGCCAATAGTTCATCGTCCGTCAAAGCGGCAGCACCGTATTTTAATAGCTTTTCCCTTGGCATTAATGC  >  1/593362‑593734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑