Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	593,337	T→C	intergenic (+208/+209)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	593,337	0	T	C	100.0%	185.4 / NA	60	intergenic (+208/+209)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/38);  total (22/38)

AGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAA  >  1/593093‑593488                                                                                                                                                                                                                                                     |                                                                                                                                                        atttgagcaagctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCAc                                                                                                                                                   <  1:23606‑M2/148‑1 (MQ=255)            ctgtagcggaaaaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAg                                                                                                                                         <  1:802821‑M2/158‑1 (MQ=255)                       aaagattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAAc                                                                                                                             <  1:21087‑M2/170‑1 (MQ=255)                          gattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAAcgg                                                                                                                           >  1:489507‑M2/79‑250 (MQ=255)                          gattaatgatagttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCCGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAacggg                                                                                                                          >  1:788641‑M2/79‑250 (MQ=255)                                      ttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAcc                                                                                                               <  1:505072‑M2/184‑1 (MQ=255)                                      ttaaaaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAc                                                                                                                <  1:460813‑M2/183‑1 (MQ=255)                                          aaataagttgactgccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGATCAGGCAGACAAAAACGGCGGGGTAATACCcgtcg                                                                                                          <  1:388202‑M2/189‑1 (MQ=255)                                                        ccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatgtt                                                                                            <  1:823710‑M2/203‑1 (MQ=255)                                                        ccggcaattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatgtt                                                                                            <  1:662066‑M2/203‑1 (MQ=255)                                                            caattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTtata                                                                                        <  1:639172‑M2/207‑1 (MQ=255)                                                             aattaaaaggattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTatat                                                                                       <  1:295208‑M2/208‑1 (MQ=255)                                                               ttactaatcaaaGGAGTAAGATTCAAACCGTCCGAGCAATCTGGCGGTTTTATGCAGCC‑GAAAGTCTGAGTGTTG‑‑‑‑AAACAGA‑CGTAACTGCTGAACAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTCATTTACTGCCGAATAGGCAgcttagaaa                                                                                                                             >  1:104402/10‑197 (MQ=255)                                                                      gattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATtc                                                                               <  1:179525‑M2/216‑1 (MQ=255)                                                                      gattaagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTTTTTATGTTTATATGATGAtt                                                                                >  1:716652‑M2/35‑249 (MQ=255)                                                                         aaGGAGTAAGATTCAAACCGTCCGAGCAATCTGGCGGTTTTATGCAGCC‑GAAAGTCTGAGTGTTG‑‑‑‑AAACAGA‑CGTAACTGCTGAACAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTCATTTACTGCCGAATAGGCAgcttagaaa                                                                                                                             >  1:462206/1‑187 (MQ=255)                                                                          aagatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGt                                                                          <  1:442944‑M2/221‑1 (MQ=255)                                                                            gatttaaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatg                                                                                              <  1:224828‑M2/201‑1 (MQ=255)                                                                                taaaatgccagagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAAtt                                                                    <  1:436222‑M2/227‑1 (MQ=255)                                                                                          gagcaatctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTTTGTTTATATGATGATTTTGGGTTAAATttgagggcgat                                                          >  1:421794‑M2/15‑247 (MQ=255)                                                                                               atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTtaaatttgagggcgattagtc                                                     <  1:824457‑M2/242‑10 (MQ=255)                                                                                               atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTtaaatttgagggcgattagtc                                                     <  1:681563‑M2/242‑10 (MQ=255)                                                                                               atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAAt                                                                     <  1:290052‑M2/226‑1 (MQ=255)                                                                                               atctggcggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCCTCGGTTTTGTTTTTGTTTTTATTATGGTTCTGCGGTtaattttagggcgattagt                                                      >  1:279353‑M2/10‑242 (MQ=255)                                                                                                     cggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTctgcgttaaatttgagggcgattagtcgttatt                                               <  1:321418‑M2/248‑16 (MQ=255)                                                                                                     cggTGGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTctgcgttaaatttgagggcgattagtcgttatt                                               <  1:859078‑M2/248‑16 (MQ=255)                                                                                                       gtggCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttattc                                              <  1:603904/247‑17 (MQ=255)                                                                                                        tggCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttattca                                             <  1:767941/248‑18 (MQ=255)                                                                                                             gTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCAc                                                                                                                                                   >  1:465789/1‑143 (MQ=255)                                                                                                             gTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttatt                                               <  1:462008/243‑16 (MQ=255)                                                                                                             gTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGAGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAgggcgattagtcgttatt                                               >  1:432932/1‑228 (MQ=255)                                                                                                                       gCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAggg                                                              <  1:691838/218‑1 (MQ=255)                                                                                                                       gCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTTTGTTTttat                                                                                       >  1:37977/1‑193 (MQ=255)                                                                                                                       gCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTTAgggcgattagtcgttatt                                               >  1:647138/1‑218 (MQ=255)                                                                                                                         cTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGt                                                                          <  1:13219/204‑1 (MQ=255)                                                                                                                         cTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTTTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAg                           >  1:793873/1‑251 (MQ=255)                                                                                                                               ggCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTtgtttatg                                                                                              >  1:56762/1‑178 (MQ=255)                                                                                                                                 cTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:126024/251‑5 (MQ=255)                                                                                                                                 cTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:622206/251‑5 (MQ=255)                                                                                                                                      tgtTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta              <  1:783339/251‑10 (MQ=255)                                                                                                                                      tgtTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta              <  1:821071/251‑10 (MQ=255)                                                                                                                                            aGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTTTTTATGTTTATATGATGATTCTGCGTTAAATTTTAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAgtgt                        >  1:322279/1‑235 (MQ=255)                                                                                                                                            aGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTTAgggcgattagtcgttatt                                               >  1:348518/1‑197 (MQ=255)                                                                                                                                              caaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaattattc      <  1:543917/251‑18 (MQ=255)                                                                                                                                               aaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAACCGTCGCTTTTGTTTTTGTTTATATGATGATTCTGCGTTAAATTTGAgggcgatttgtcgttatt                                               >  1:649193/1‑194 (MQ=255)                                                                                                                                               aaacaaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCATCGTTTTTGTTTATGTTTATATGATGATTTTGCGTTAAATTTTAGGGGGGTTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   >  1:158898/1‑233 (MQ=255)                                                                                                                                                  caaaGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaattattcatac  <  1:859192/251‑22 (MQ=255)                                                                                                                                                     gacgtAACTGCTGAACAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTCATTTACTGCCGaat                                                                                                                                           <  1:100385/107‑2 (MQ=255)                                                                                                                                                            ctgctgAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATAc                                                                                                                >  1:642986/1‑131 (MQ=255)                                                                                                                                                              gctgAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaatta         <  1:482745/232‑15 (MQ=255)                                                                                                                                                                 gAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTTTGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   >  1:826309/1‑215 (MQ=255)                                                                                                                                                                          gcTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATa                                                                                                                 <  1:460553/116‑1 (MQ=255)                                                                                                                                                                          gcTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATatgat                                                                                    >  1:155728/1‑145 (MQ=255)                                                                                                                                                                             tAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATatgat                                                                                    <  1:51227/142‑1 (MQ=255)                                                                                                                                                                                    tGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGGAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgtt                    >  1:775368/1‑196 (MQ=255)                                                                                                                                                                                                             gCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttatta              >  1:440256/1‑171 (MQ=255)                                                                                                                                                                                                                                aaaTAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttt                   <  1:258670/156‑5 (MQ=255)                                                                                                                                                                                                                                  aTAGTAGTTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaat           <  1:284319/162‑13 (MQ=255)                                                                                                                                                                                                                                        gtTAAGCTCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATtc                                                                               <  1:191995/88‑1 (MQ=255)                                                                                                                                                                                                                                             gctCTACTCATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTGCGTTAAATTTGAGGGCGATTAGTCGTTATTCATGTGTAAAATTTACGTAGTGttgttttattaaat           <  1:366774/149‑13 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                        AGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTGTTTATGTTTATATGATGATTCTCCGTTAAATTTGAGGGTTGTTAGTTGTTGGTGATGTGTAAAATTTACGTAGTGTTACTTAATTTAAAACCATATAA  >  1/593093‑593488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑