Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	593,150	G→A	intergenic (+21/+396)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	593,150	0	G	A	100.0%	220.7 / NA	78	intergenic (+21/+396)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/34);  total (44/34)

ATCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTG  >  1/592914‑593390                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 aTCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTaa                                                                                                                                                                                                                                    >  1:533372/1‑251 (MQ=255)     aaaGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAgg                                                                                                                                                                                                                                <  1:466433/251‑1 (MQ=255)        gtgtAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGatt                                                                                                                                                                                                                             >  1:292677/1‑249 (MQ=255)        gtgtAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGatt                                                                                                                                                                                                                             >  1:280020/1‑249 (MQ=255)             gCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGAt                                                                                                                                                                                                                        >  1:722171/1‑251 (MQ=255)              cTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGa                                                                                                                                                                                                                         <  1:761686/249‑1 (MQ=255)                    aTGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaat                                                                                                                                                                                                                 >  1:292006/1‑250 (MQ=255)                             atCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGCAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaaatgccagag                                                                                                                                                                                                         >  1:47801/1‑241 (MQ=255)                                   atgaCATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtgg                                                                                                                                                                                                  >  1:260116‑M1/1‑248 (MQ=255)                                                cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaa                                                                                                                                                                                                                  >  1:743201/1‑222 (MQ=255)                                                cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggtttt                                                                                                                                                                                     >  1:355614‑M1/1‑235 (MQ=255)                                                    aTCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                                 <  1:488391‑M1/251‑21 (MQ=255)                                                     tCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGc                                                                                                                                                                                                         <  1:645136/226‑1 (MQ=255)                                                        cTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTCCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTCATGATAGTTAAAAATACGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagc                                                                                                                                                                              >  1:222643‑M1/1‑227 (MQ=255)                                                          gCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                                 >  1:240334‑M1/1‑225 (MQ=255)                                                             gATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaa                                                                                                                                                                        >  1:278964‑M1/1‑222 (MQ=255)                                                                  cGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                                 <  1:309368‑M1/237‑21 (MQ=255)                                                                    cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgag                                                                                                                                                                 >  1:366439‑M1/1‑215 (MQ=255)                                                                    cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctg                                                                                                                                                                   >  1:144640‑M1/1‑215 (MQ=255)                                                                             cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaag                                                                                                                                                         <  1:238959‑M1/250‑45 (MQ=255)                                                                             cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaag                                                                                                                                                         <  1:547566‑M1/250‑45 (MQ=255)                                                                                   aagaaAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                                 >  1:537335‑M1/1‑200 (MQ=255)                                                                                       aaGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaag                                                                                                                                                >  1:811874‑M1/1‑196 (MQ=255)                                                                                            ggTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaa                                                                                                                                           >  1:111492‑M1/1‑191 (MQ=255)                                                                                              tATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCt                                                                                                                                                                                                    <  1:77592‑M1/190‑2 (MQ=255)                                                                                                      cTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                                               >  1:464298‑M1/1‑181 (MQ=255)                                                                                                       tATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATGTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                                              <  1:450445‑M1/251‑72 (MQ=255)                                                                                                        aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                                             >  1:557638‑M1/1‑179 (MQ=255)                                                                                                        aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                                             >  1:869732‑M1/1‑179 (MQ=255)                                                                                                               agaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttag                                                                                                                       <  1:15288‑M1/250‑79 (MQ=255)                                                                                                                gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgc                                                                                                                                       >  1:746604‑M1/1‑171 (MQ=255)                                                                                                                gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaa                                                                                                                                                  <  1:136009‑M1/222‑52 (MQ=255)                                                                                                                 aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                    >  1:709535‑M1/1‑170 (MQ=255)                                                                                                                 aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                    <  1:830664‑M1/251‑82 (MQ=255)                                                                                                                 aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                    <  1:793022‑M1/251‑82 (MQ=255)                                                                                                                    gtgCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                                               <  1:498614‑M1/237‑71 (MQ=255)                                                                                                                      aagagacatcacttggattttCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggt                                                                                                               <  1:412469‑M1/242‑87 (MQ=255)                                                                                                                               cacTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagc                                                                                                                                               >  1:571215‑M1/1‑156 (MQ=255)                                                                                                                                      aTTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATGAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttacggaaag                                                                                                <  1:448613‑M1/250‑102 (MQ=255)                                                                                                                                           ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttca                                                                                            >  1:652735‑M1/2‑144 (MQ=255)                                                                                                                                           ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                    >  1:368206‑M1/2‑144 (MQ=255)                                                                                                                                           ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctaaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                                              >  1:453487‑M1/2‑144 (MQ=255)                                                                                                                                                aGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcct                                                                                                                                                                            <  1:435185‑M1/164‑26 (MQ=255)                                                                                                                                                  aTGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                   <  1:729806‑M1/251‑115 (MQ=255)                                                                                                                                                             aaaTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCagag                                                                                                                                                                                                          >  1:484286/1‑121 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                      >  1:28088‑M1/1‑124 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                      >  1:74929‑M1/1‑124 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                      >  1:513870‑M1/1‑124 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                                      >  1:367057‑M1/1‑124 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctc                                                                                                          >  1:84616‑M1/1‑124 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctg                                                                                                                                     <  1:476758‑M1/188‑65 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaag                                                                                                                                                         <  1:816764‑M1/168‑45 (MQ=255)                                                                                                                                                               aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaagg                                                                                                                                                                      >  1:676878‑M1/1‑124 (MQ=255)                                                                                                                                                                tttAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaa                                                                     <  1:730728‑M1/251‑129 (MQ=255)                                                                                                                                                                    aTAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatag                                                                 <  1:720344‑M1/251‑133 (MQ=255)                                                                                                                                                                        agaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataa                                                                                                                                >  1:182497‑M1/1‑115 (MQ=255)                                                                                                                                                                         gaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                   >  1:419665‑M1/1‑114 (MQ=255)                                                                                                                                                                               gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagct                                                       <  1:379077‑M1/247‑143 (MQ=255)                                                                                                                                                                               gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaat                                                                   >  1:251016‑M1/4‑108 (MQ=255)                                                                                                                                                                                cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctc                                                      <  1:156422‑M1/248‑144 (MQ=255)                                                                                                                                                                                cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaa                                                                                                  <  1:115508‑M1/204‑100 (MQ=255)                                                                                                                                                                                  ttAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctac                                                   <  1:168102‑M1/251‑147 (MQ=255)                                                                                                                                                                                    aGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctact                                                  >  1:348389‑M1/1‑103 (MQ=255)                                                                                                                                                                                      atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                                 <  1:590268‑M1/249‑149 (MQ=255)                                                                                                                                                                                      atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                                 <  1:858124‑M1/249‑149 (MQ=255)                                                                                                                                                                                          atttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcac                                           <  1:23606‑M1/251‑155 (MQ=255)                                                                                                                                                                                                   aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagc                                                        >  1:24663‑M1/1‑88 (MQ=255)                                                                                                                                                                                                   aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgc                                                                                        >  1:719171‑M1/1‑88 (MQ=255)                                                                                                                                                                                                     cTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacag                                 <  1:802821‑M1/250‑165 (MQ=255)                                                                                                                                                                                                            ggAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                                   >  1:137816‑M1/1‑79 (MQ=255)                                                                                                                                                                                                                aaaGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaac                     <  1:21087‑M1/251‑177 (MQ=255)                                                                                                                                                                                                                  aGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagc                                                                            >  1:234151‑M1/1‑73 (MQ=255)                                                                                                                                                                                                                   gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacgg                   >  1:489507‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                   gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggccgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggg                  >  1:788641‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                               ttAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatacc       <  1:505072‑M1/250‑191 (MQ=255)                                                                                                                                                                                                                               ttAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggcggggtaatac        <  1:460813‑M1/249‑190 (MQ=255)                                                                                                                                                                                                                                   aaaTAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgatcaggcagacaaaaacggcggggtaatacccgtcg  <  1:388202‑M1/251‑196 (MQ=255)                                                                                                                                                                                                                                    aaTAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                                    >  1:292646‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 ATCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTAATACCCGTCGTTTTTG  >  1/592914‑593390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑