Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	593,133	C→T	intergenic (+4/+413)	unknown → / ← unknown	hypothetical protein/UPF0758 protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	593,133	0	C	T	100.0%	237.8 / NA	82	intergenic (+4/+413)	unknown/unknown	hypothetical protein/UPF0758 protein YicR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/35);  total (47/35)

GATTTGAAATCTTTCATACAATCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTA  >  1/592894‑593374                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  gATTTGAAATCTTTCATACAATCAAAAGTGTAGCTGATTATGTAAATAATCGAGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATa                                                                                                                                                                                                                                        >  1:512922/1‑251 (MQ=255)    ttGAAATCTTTCATACAATCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGt                                                                                                                                                                                                                                     <  1:576991/251‑1 (MQ=255)       aaaTCTTTCATACAATCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTg                                                                                                                                                                                                                                   <  1:209164/250‑1 (MQ=255)                     aTCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAAt                                                                                                                                                                                                                                         <  1:215529/230‑1 (MQ=255)                     aTCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTaa                                                                                                                                                                                                                    >  1:533372/1‑251 (MQ=255)                         aaaGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAgg                                                                                                                                                                                                                <  1:466433/251‑1 (MQ=255)                            gtgtAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGatt                                                                                                                                                                                                             >  1:292677/1‑249 (MQ=255)                            gtgtAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGatt                                                                                                                                                                                                             >  1:280020/1‑249 (MQ=255)                                 gCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGAt                                                                                                                                                                                                        >  1:722171/1‑251 (MQ=255)                                  cTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGa                                                                                                                                                                                                         <  1:761686/249‑1 (MQ=255)                                        aTGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaat                                                                                                                                                                                                 >  1:292006/1‑250 (MQ=255)                                          gTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAAt                                                                                                                                                                                                                                         >  1:675751/1‑209 (MQ=255)                                                 atCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGCAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaaatgccagag                                                                                                                                                                                         >  1:47801/1‑241 (MQ=255)                                                   cGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAAt                                                                                                                                                                                                                                         >  1:202055/1‑200 (MQ=255)                                                       atgaCATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtgg                                                                                                                                                                                  >  1:260116‑M1/1‑248 (MQ=255)                                                                    cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTaaaa                                                                                                                                                                                                  >  1:743201/1‑222 (MQ=255)                                                                    cATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggtttt                                                                                                                                                                     >  1:355614‑M1/1‑235 (MQ=255)                                                                        aTCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                 <  1:488391‑M1/251‑21 (MQ=255)                                                                         tCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGc                                                                                                                                                                                         <  1:645136/226‑1 (MQ=255)                                                                            cTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTCCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTCATGATAGTTAAAAATACGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagc                                                                                                                                                              >  1:222643‑M1/1‑227 (MQ=255)                                                                              gCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                 >  1:240334‑M1/1‑225 (MQ=255)                                                                                 gATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaa                                                                                                                                                        >  1:278964‑M1/1‑222 (MQ=255)                                                                                      cGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                 <  1:309368‑M1/237‑21 (MQ=255)                                                                                        cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgag                                                                                                                                                 >  1:366439‑M1/1‑215 (MQ=255)                                                                                        cTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctg                                                                                                                                                   >  1:144640‑M1/1‑215 (MQ=255)                                                                                                 cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaag                                                                                                                                         <  1:238959‑M1/250‑45 (MQ=255)                                                                                                 cATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaag                                                                                                                                         <  1:547566‑M1/250‑45 (MQ=255)                                                                                                       aagaaAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgc                                                                                                                                                                 >  1:537335‑M1/1‑200 (MQ=255)                                                                                                           aaGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaag                                                                                                                                >  1:811874‑M1/1‑196 (MQ=255)                                                                                                                ggTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaa                                                                                                                           >  1:111492‑M1/1‑191 (MQ=255)                                                                                                                  tATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCt                                                                                                                                                                                    <  1:77592‑M1/190‑2 (MQ=255)                                                                                                                          cTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTgac                                                                                                                                                                                                                                 >  1:556660/1‑135 (MQ=255)                                                                                                                          cTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                               >  1:464298‑M1/1‑181 (MQ=255)                                                                                                                           tATTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATGTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                              <  1:450445‑M1/251‑72 (MQ=255)                                                                                                                            aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGt                                                                                                                                                                                                                                     <  1:323485/131‑1 (MQ=255)                                                                                                                            aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                             >  1:869732‑M1/1‑179 (MQ=255)                                                                                                                            aTTCGAGAGAATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagca                                                                                                             >  1:557638‑M1/1‑179 (MQ=255)                                                                                                                                   agaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttag                                                                                                       <  1:15288‑M1/250‑79 (MQ=255)                                                                                                                                    gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgc                                                                                                                       >  1:746604‑M1/1‑171 (MQ=255)                                                                                                                                    gaATGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaa                                                                                                                                  <  1:136009‑M1/222‑52 (MQ=255)                                                                                                                                     aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                    <  1:830664‑M1/251‑82 (MQ=255)                                                                                                                                     aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                    <  1:793022‑M1/251‑82 (MQ=255)                                                                                                                                     aaTGTGCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                    >  1:709535‑M1/1‑170 (MQ=255)                                                                                                                                        gtgCAGACGCACACTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataag                                                                                                               <  1:498614‑M1/237‑71 (MQ=255)                                                                                                                                          aagagacatcacttggattttCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggt                                                                                               <  1:412469‑M1/242‑87 (MQ=255)                                                                                                                                                   cacTTGGATTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagc                                                                                                                               >  1:571215‑M1/1‑156 (MQ=255)                                                                                                                                                          aTTTTCTACAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATGAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttacggaaag                                                                                <  1:448613‑M1/250‑102 (MQ=255)                                                                                                                                                               ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttca                                                                            >  1:652735‑M1/2‑144 (MQ=255)                                                                                                                                                               ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaa                                                                                                    >  1:368206‑M1/2‑144 (MQ=255)                                                                                                                                                               ctaCAAGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctaaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagc                                                                                                              >  1:453487‑M1/2‑144 (MQ=255)                                                                                                                                                                    aGATGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcct                                                                                                                                                            <  1:435185‑M1/164‑26 (MQ=255)                                                                                                                                                                      aTGCGGGAGCTAAATTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                   <  1:729806‑M1/251‑115 (MQ=255)                                                                                                                                                                                 aaaTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCagag                                                                                                                                                                                          >  1:484286/1‑121 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                      >  1:28088‑M1/1‑124 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                      >  1:513870‑M1/1‑124 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                      >  1:367057‑M1/1‑124 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaa                                                      >  1:74929‑M1/1‑124 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctc                                                                                          >  1:84616‑M1/1‑124 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctg                                                                                                                     <  1:476758‑M1/188‑65 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaag                                                                                                                                         <  1:816764‑M1/168‑45 (MQ=255)                                                                                                                                                                                   aTTTAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaagg                                                                                                                                                      >  1:676878‑M1/1‑124 (MQ=255)                                                                                                                                                                                    tttAATAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaa                                                     <  1:730728‑M1/251‑129 (MQ=255)                                                                                                                                                                                        aTAGAGATGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatag                                                 <  1:720344‑M1/251‑133 (MQ=255)                                                                                                                                                                                            agaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataa                                                                                                                >  1:182497‑M1/1‑115 (MQ=255)                                                                                                                                                                                             gaTGAGGCATTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                   >  1:419665‑M1/1‑114 (MQ=255)                                                                                                                                                                                                   gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagct                                       <  1:379077‑M1/247‑143 (MQ=255)                                                                                                                                                                                                   gcattagaTTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaat                                                   >  1:251016‑M1/4‑108 (MQ=255)                                                                                                                                                                                                    cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctc                                      <  1:156422‑M1/248‑144 (MQ=255)                                                                                                                                                                                                    cattagATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaa                                                                                  <  1:115508‑M1/204‑100 (MQ=255)                                                                                                                                                                                                      ttAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctac                                   <  1:168102‑M1/251‑147 (MQ=255)                                                                                                                                                                                                        aGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctact                                  >  1:348389‑M1/1‑103 (MQ=255)                                                                                                                                                                                                          atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                 <  1:590268‑M1/249‑149 (MQ=255)                                                                                                                                                                                                          atttatttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactc                                 <  1:858124‑M1/249‑149 (MQ=255)                                                                                                                                                                                                              atttGAGCAAGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcac                           <  1:23606‑M1/251‑155 (MQ=255)                                                                                                                                                                                                                       aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagc                                        >  1:24663‑M1/1‑88 (MQ=255)                                                                                                                                                                                                                       aGCTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgc                                                                        >  1:719171‑M1/1‑88 (MQ=255)                                                                                                                                                                                                                         cTGTAGCGGAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacag                 <  1:802821‑M1/250‑165 (MQ=255)                                                                                                                                                                                                                                ggAAAAAGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaac                                                                   >  1:137816‑M1/1‑79 (MQ=255)                                                                                                                                                                                                                                    aaaGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaac     <  1:21087‑M1/251‑177 (MQ=255)                                                                                                                                                                                                                                      aGATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagc                                                            >  1:234151‑M1/1‑73 (MQ=255)                                                                                                                                                                                                                                       gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggctgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacgg   >  1:489507‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                                       gATTAATGATAGTTAAAAATAAGTTGACTGCCGGCAATTAAAAGGATTAAGATTTAAAATGCCAGAGCAATCtggcggtggcggttttatgcagcctgaaaggccgagtgttgaagcaaacaaagcttaactgctgaataagcagcttagaaaatggtagctcttactgaaagttcactgccgaacaggcagcttagaaaatagtagttaagctctactcattcactgccgaacaggcagacaaaaacggg  >  1:788641‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  GATTTGAAATCTTTCATACAATCAAAAGTGTAGCTGATTATGTAAATAATCGTGATGACATTGCGGACATTATCGCTGCAGATTACGCTTTAATCGCATGCGAAGAAAGCGGGTATGATGACTATTCGAGAGAATGTGCAGACGCACACTTGGATTTTTTACAAGATGCTGGAGCTAAATTTAATAGAGATGGGGCGTTAGATTTATTTGAGCAAGCTGTAGCGGAAAAAGATTAATGACAGTTAAAAATAAGTTGGCTGCCGGCAATTAAAAGGAGTAAGATTTAAAACGCCAGAGCAATCTAGCGGTTTTATGCAGCCTGAAAGGCTGAGTGTTGAAGCAAACAAAGCTTAACTGCTGAATAAGCAGCTTAGAAAATGGTAGCTCTTACTGAAAGTTCACTGCCGAACAGGCAGCTTAGAAAATAGTAGTTAAGTTCTACTTATTCACTGCCGAACAGGCAGACAAAAACGGCGGGGTA  >  1/592894‑593374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑