Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	588,333	A→C	N259H (AAT→CAT)	unknown →	COG0859: ADP‑heptose:LPS heptosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	588,333	0	A	C	100.0%	120.0 / NA	40	N259H (AAT→CAT)	unknown	COG0859: ADP‑heptose:LPS heptosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/26);  total (14/26)

TTCCAAATCACAGTGATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAAAATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACATATATCCTTAATTTTATTGAAAATGTCAATGAAATCTCTCCCGATGAAAT  >  1/588088‑588559                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   ttCCAAATCACAGTGATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATa                                                                                                                                                                                                                                 >  1:164317/1‑249 (MQ=255) ttCCAAATCACAGTGATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATAc                                                                                                                                                                                                                                <  1:255911/250‑1 (MQ=255)               gATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCATAAAAATATACCAATGCTTTTATCTATGAACATAc                                                                                                                                                                                                                                >  1:253949/1‑236 (MQ=255)               gATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGaaaa                                                                                                                                                                                                                          <  1:2916/242‑1 (MQ=255)               gATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGa                                                                                                                                                                                                                 >  1:858419/1‑251 (MQ=255)               gATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGa                                                                                                                                                                                                                 >  1:561100/1‑251 (MQ=255)                             tatTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTAc                                                                                                                                                                                                   <  1:44877/251‑1 (MQ=255)                                       ttCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATAc                                                                                                                                                                                         <  1:93013/251‑1 (MQ=255)                                       ttCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACAATAACATTACTTCATTATAc                                                                                                                                                                                         <  1:126128/251‑1 (MQ=255)                                            aCAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    <  1:692165/251‑1 (MQ=255)                                            aCAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    <  1:829364/251‑1 (MQ=255)                                            aCAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATCTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    <  1:437880/251‑1 (MQ=255)                                             cAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATAc                                                                                                                                                                                         <  1:231488/245‑1 (MQ=255)                                                 aaaaTCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGtt                                                                                                                                                                               <  1:239629/251‑1 (MQ=255)                                                        tcTTGTCGATTGCATCGCTATAAACTTTTTTAGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTtctc                                                                                                                                                                        <  1:874651/251‑1 (MQ=255)                                                               gATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGa                                                                                                                                                                                                                             >  1:816949/1‑191 (MQ=255)                                                                   gCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATAc                                                                                                                                                                                         >  1:315627/1‑223 (MQ=255)                                                                         cTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTattatt                                                                                                                                                       >  1:177315/1‑251 (MQ=255)                                                                         cTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTattatt                                                                                                                                                       >  1:334497/1‑251 (MQ=255)                                                                          tataAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTc                                                                                                                                                      <  1:369488/251‑1 (MQ=255)                                                                           ataAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    <  1:552720/220‑1 (MQ=255)                                                                                cTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAACGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    <  1:188071/215‑1 (MQ=255)                                                                                                                            tttATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    <  1:761241/171‑1 (MQ=255)                                                                                                                              tATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTc                                                                                                                                                                                                <  1:295120/157‑1 (MQ=255)                                                                                                                                     ttATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGaa                                                                                           <  1:62202/251‑1 (MQ=255)                                                                                                                                                                          gTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACAt                                                                                                                                                                                                      <  1:471272/107‑1 (MQ=255)                                                                                                                                                                           ttACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTg                                                                                                                                                                                                                  <  1:232410/94‑1 (MQ=255)                                                                                                                                                                              cTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACata                                                  >  1:98391/1‑251 (MQ=255)                                                                                                                                                                                aaCCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGcc                                                                                                                                                                                    >  1:826084/1‑119 (MQ=255)                                                                                                                                                                                     aCCCCGAAGTAACCCCTTTGTTAAAAACTATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCatat                                                                                                                                                  >  1:589443/1‑148 (MQ=255)                                                                                                                                                                                     aCCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACATATATCCtt                                           >  1:405329/1‑251 (MQ=255)                                                                                                                                                                                          gAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACATATATCCTTAAttt                                      >  1:437868/1‑251 (MQ=255)                                                                                                                                                                                                                              tataCCAATGCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACATATATCCTTAATTTTATTGAAAATGTCAATGAAATCTCTCCCGATGAAAt  <  1:349406/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATAc                                                                                                                                                                                         >  1:663745/1‑59 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCat                                                                                                                                                    <  1:561432/96‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTtat                                                                                                             <  1:353374/135‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGc                                                                                 <  1:754929/163‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAAc                                                                   <  1:714445/177‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTg                                                                 <  1:50423/179‑1 (MQ=255)                                                                                                                                                                                                                                       gCTTTTATCTATGAACATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACATATATCCTTAAtt                                       <  1:274325/205‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                   TTCCAAATCACAGTGATAAAAAAGAAGATATTGCAAATTTCATACAAAAAAATCATCTTGTCGATTGCATCGCTATAAACTTTTTTGGTGCAGCCGGCACCAGAAAATTTACGGAACAAAACATTTATCGGTTTATCGAAAAATTTAAAGCCGAAAATAAAAAAGCGCTGTTACTAACCTACCCCGAAGTAACCCCTTTGTTAAAAACGATAGCAGAAAAATATACCAATGCTTTTATCTATGAAAATACTGAAAATATCTTTGATACGATAACATTACTTCATTATACTAGCCTCGTTATTTCTCCTGATACGTCTATTATTCATATTGCAGCAGGATTAAATAAAAAAATTATTGGATTTTATAAGCTCGCTGATAAAGAAAACTTTACGCATTGGAATCCAAACTGTAAAAATAAAACATATATCCTTAATTTTATTGAAAATGTCAATGAAATCTCTCCCGATGAAAT  >  1/588088‑588559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑