Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	583,186	T→C	L63L (CTA→CTG)	unknown ←	Helicase PriA essential for oriC/DnaA‑independent DNA replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	583,186	0	T	C	100.0%	271.3 / NA	90	L63L (CTA→CTG)	unknown	Helicase PriA essential for oriC/DnaA‑independent DNA replication
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/54);  total (36/54)

CCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCTAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATAAAATGGGCTCGGAATATAGC  >  1/582942‑583415                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       ccgccTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:326207/250‑1 (MQ=255) ccgccTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:685458/250‑1 (MQ=255) ccgccTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:387046/250‑1 (MQ=255) ccgccTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:117934/250‑1 (MQ=255) ccgccTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTGTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:424792/250‑1 (MQ=255)     cTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:156626/246‑1 (MQ=255)          ccGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAATACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:434506/250‑1 (MQ=255)          aCGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCTGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:496581/251‑1 (MQ=255)          aCGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:11865/251‑1 (MQ=255)          aCGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:358732/251‑1 (MQ=255)          aCGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:383964/251‑1 (MQ=255)          aCGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:467525/251‑1 (MQ=255)          aCGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:1421/251‑1 (MQ=255)                       cttcttCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGca                                                                                                                                                                                                            <  1:888515/251‑1 (MQ=255)                         tcttcGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         >  1:423657/1‑236 (MQ=255)                             cgcgcgTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCg                                                                                                                                                                                                      <  1:399018/251‑1 (MQ=255)                                   tttCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         >  1:64737/1‑226 (MQ=255)                                     tCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:690126/224‑1 (MQ=255)                                     tCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTc                                                                                                                                                                                               >  1:336076/1‑250 (MQ=255)                                     tCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTc                                                                                                                                                                                               >  1:790831/1‑250 (MQ=255)                                      cAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCg                                                                                                                                                                                             >  1:502937/1‑251 (MQ=255)                                       aaTAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         >  1:245944/1‑222 (MQ=255)                                       aaTAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCg                                                                                                                                                                                             >  1:304513/1‑250 (MQ=255)                                       aaTAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGc                                                                                                                                                                                            <  1:199068/251‑1 (MQ=255)                                            gCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         >  1:765803/1‑217 (MQ=255)                                            gCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         >  1:2331/1‑217 (MQ=255)                                             cccTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCt                                                                                                                                                                                       <  1:828139/250‑1 (MQ=255)                                                 gcgcCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAac                                                                                                                                                                                   >  1:37720/1‑250 (MQ=255)                                                  cgcCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   >  1:611438/1‑201 (MQ=255)                                                  cgcCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAacc                                                                                                                                                                                  <  1:762061/250‑1 (MQ=255)                                                   gcCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:723683/210‑1 (MQ=255)                                                   gcCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAAGTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:787586/200‑1 (MQ=255)                                                    ccAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAaccac                                                                                                                                                                                <  1:786047/250‑1 (MQ=255)                                                        tGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGc                                                                                                                                                                           <  1:416032/251‑1 (MQ=255)                                                           cTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAaccac                                                                                                                                                                                <  1:616179/243‑1 (MQ=255)                                                            tGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCaa                                                                                                                                                                        >  1:139510/1‑250 (MQ=255)                                                                cACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:705874/187‑1 (MQ=255)                                                                cACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGc                                                                                                                                                                                                             >  1:207538/1‑209 (MQ=255)                                                                cACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACg                                                                                                                                                                    >  1:874209/1‑250 (MQ=255)                                                                cACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACg                                                                                                                                                                    >  1:701903/1‑250 (MQ=255)                                                                  ccAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACt                                                                                                                                                                                                   <  1:2348/217‑1 (MQ=255)                                                                      gTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:770300/181‑1 (MQ=255)                                                                         cggtcggtTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTgcgc                                                                                                                                                           <  1:598976/250‑1 (MQ=255)                                                                              ggtTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTc                                                                                                                                                                                                                    >  1:380290/1‑188 (MQ=255)                                                                                   cccaagtgttttttggaaaccgaCCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCa                                                                                                                                                >  1:252135/23‑251 (MQ=255)                                                                                   ccAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   >  1:760944/1‑168 (MQ=255)                                                                                   ccAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGAc                                                                                                                                                                                                    <  1:205413/199‑1 (MQ=255)                                                                                                 ggTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAacc                                                                                                                                                                                  >  1:875206/1‑203 (MQ=255)                                                                                                             cACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAacc                                                                                                                                                                                  <  1:591288/191‑1 (MQ=255)                                                                                                               cTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACC‑CATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCAtt                                                                                                                   >  1:142087/1‑250 (MQ=255)                                                                                                                         gCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAacca                                                                                                                                                                                 >  1:171926/1‑180 (MQ=255)                                                                                                                                        gCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATc                                                                                           <  1:146798/250‑1 (MQ=255)                                                                                                                                        gCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATc                                                                                           <  1:73066/250‑1 (MQ=255)                                                                                                                                              ccTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAg                                                                                    <  1:882583/251‑1 (MQ=255)                                                                                                                                                  cGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAAt                                                                                      <  1:402541/245‑1 (MQ=255)                                                                                                                                                  cGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGa                                                                                 <  1:452921/250‑1 (MQ=255)                                                                                                                                                  cGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGa                                                                                 <  1:427635/250‑1 (MQ=255)                                                                                                                                                  cGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGAt                                                                                >  1:59698/1‑251 (MQ=255)                                                                                                                                                       acacTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAAc                                                                           <  1:531304/251‑1 (MQ=255)                                                                                                                                                            tCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATc                                                                                           <  1:152683/230‑1 (MQ=255)                                                                                                                                                             cccccAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCt                                                                                                                                      <  1:581230/187‑1 (MQ=255)                                                                                                                                                             cccccAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAAt                                                                                            >  1:489376/1‑228 (MQ=255)                                                                                                                                                             cccccAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGcaca                                                                     >  1:867413/1‑251 (MQ=255)                                                                                                                                                              ccccAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTATCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGc                                                                                                               >  1:64264/1‑208 (MQ=255)                                                                                                                                                                ccAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGa                                                                  <  1:599089/251‑1 (MQ=255)                                                                                                                                                                 cAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGaa                                                                 <  1:471265/251‑1 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:552545/80‑1 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAAc                                                                                                                                                                                                                         <  1:51456/90‑1 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACAccc                                                                                                                                       <  1:858813/172‑1 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGc                                                                                                               >  1:229565/1‑195 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCg                                                       >  1:892435/1‑251 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCg                                                       >  1:245259/1‑251 (MQ=255)                                                                                                                                                                           tGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCg                                                       >  1:780210/1‑251 (MQ=255)                                                                                                                                                                             ataataCCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGa                                                                                 >  1:392998/1‑223 (MQ=255)                                                                                                                                                                             ataataCCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCggg                                                     >  1:311922/1‑251 (MQ=255)                                                                                                                                                                                 taCCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGa                                                                                 >  1:609835/1‑219 (MQ=255)                                                                                                                                                                                  aCCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAAt                                                                                      >  1:587434/1‑213 (MQ=255)                                                                                                                                                                                           cccACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGaa                                        <  1:265747/250‑1 (MQ=255)                                                                                                                                                                                               cTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCAc                                                                                                                                                                                                                                   <  1:270842/60‑1 (MQ=255)                                                                                                                                                                                                 gCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAAt                                                                                      >  1:516400/1‑198 (MQ=255)                                                                                                                                                                                                        cTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAac                                                                                                                                                                       <  1:852409/111‑1 (MQ=255)                                                                                                                                                                                                                  tGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATAAAATgg                <  1:250455/251‑1 (MQ=255)                                                                                                                                                                                                                             tAAGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGc                                                                                   <  1:304763/173‑1 (MQ=255)                                                                                                                                                                                                                              aaGGAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCGCCCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATaa                     >  1:285116/1‑235 (MQ=255)                                                                                                                                                                                                                                 gAAACCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATAAAATGGGCTCGGAATATAGc  <  1:384687/250‑1 (MQ=255)                                                                                                                                                                                                                                 gAAACCACATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATAAAATGGGCTCGGAATATAGc  <  1:196446/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaCCTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGc                                                                                                                      >  1:840136/1‑132 (MQ=255)                                                                                                                                                                                                                                     ccTCATCAATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATAAAATggg               <  1:283435/233‑1 (MQ=255)                                                                                                                                                                                                                                        catcaATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGc                                                                                                               <  1:432604/134‑1 (MQ=255)                                                                                                                                                                                                                                         atcaATGCAAGCCAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGa                                                                  <  1:361616/178‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       CCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCACCGCACTTTCGCCCTGCCGCAATTTCACCGGCAACGCCTGCGACAACACTTCCCCCAAGGGCGCGTGATAATACCCCGCCGCCCACTGCAAAAACTGCCAGCTTTGCGGAGAAAATAAGGAAACCTCATCAATGCAAGCTAGCACTGTCTTCAACTGCTCCGCAGGCACATCCGACTGCTCCGCCACCTCAACCACCATGCCAACACGCTTCTGCGCCCCAAACGGCACCCACACCCTCG‑CCCCCACGACAGCATTCAGCGACGCCGGCAGGATATAATCAAAATAGCGATGTAACGGCACAGGAAGCGCAATTCGGGCAAATGACATGAACGGAAATTCAGTGAAATAAAATGGGCTCGGAATATAGC  >  1/582942‑583415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑