Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	582,813	T→C	N188D (AAT→GAT)	unknown ←	Helicase PriA essential for oriC/DnaA‑independent DNA replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	582,813	0	T	C	100.0%	267.7 / NA	91	N188D (AAT→GAT)	unknown	Helicase PriA essential for oriC/DnaA‑independent DNA replication
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/49);  total (42/49)

TTAAAACGCGCCTGAAAACGCCGCACCGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATTGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCAC  >  1/582566‑583047                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           ttAAAACGCGCCTGAAAACGCCGCACCGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGcc                                                                                                                                                                                                                                         <  1:901774/251‑1 (MQ=255)       cgcgCCTGAAAACGCCGCACCGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAAttg                                                                                                                                                                                                                                   <  1:437855/251‑1 (MQ=255)           ccTGAAAACGCCGCACCGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAAttgttg                                                                                                                                                                                                                                <  1:806191/250‑1 (MQ=255)              gAAAACGCCGCACCGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTtgctg                                                                                                                                                                                                                             >  1:412390/1‑250 (MQ=255)                          ccGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAAttgttg                                                                                                                                                                                                                                <  1:218563/235‑1 (MQ=255)                          ccGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGAtt                                                                                                                                                                                                                >  1:524802/1‑251 (MQ=255)                                    cGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTcc                                                                                                                                                                                                       <  1:653315/250‑1 (MQ=255)                                             gATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGc                                                                                                                                                                                                  >  1:273425/1‑246 (MQ=255)                                             gATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACtt                                                                                                                                                                                             >  1:323469/1‑251 (MQ=255)                                                   cGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCTGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGa                                                                                                                                                                                       <  1:430331/251‑1 (MQ=255)                                                          aaCACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGa                                                                                                                                                                                       <  1:364082/244‑1 (MQ=255)                                                            cacCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAAttg                                                                                                                                                                                                                                   <  1:287722/198‑1 (MQ=255)                                                            cacCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGAtt                                                                                                                                                                                                                >  1:847354/1‑217 (MQ=255)                                                                         ttgccttgcTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAgcgc                                                                                                                                                                 <  1:172076/251‑1 (MQ=255)                                                                                  tgcAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAAt                                                                                                                                                        >  1:760969/1‑251 (MQ=255)                                                                                    cAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATc                                                                                                                                                       <  1:179022/250‑1 (MQ=255)                                                                                     aaaaTCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATcgc                                                                                                                                                     <  1:683637/251‑1 (MQ=255)                                                                                          ctctTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCAc                                                                                                                                                <  1:166552/251‑1 (MQ=255)                                                                                            ctTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTa                                                                                                                                              >  1:637928/1‑251 (MQ=255)                                                                                                 aTAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAg                                                                                                                                          <  1:604068/250‑1 (MQ=255)                                                                                                  tAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:177991/251‑1 (MQ=255)                                                                                                  tAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:838103/251‑1 (MQ=255)                                                                                                  tAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:440786/251‑1 (MQ=255)                                                                                                  tAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:514967/251‑1 (MQ=255)                                                                                                  tAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:744204/251‑1 (MQ=255)                                                                                                  tAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:256571/251‑1 (MQ=255)                                                                                                      ttGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGgttgt                                                                                                                                    >  1:355925/1‑251 (MQ=255)                                                                                                         cAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGc                                                                                                                                 <  1:76718/251‑1 (MQ=255)                                                                                                            aTAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTtgc                                                                                                                                                                                                                               <  1:198557/154‑1 (MQ=255)                                                                                                               gATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAg                                                                                                                                          >  1:289529/1‑236 (MQ=255)                                                                                                                aTTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTtctc                                                                                                                          <  1:59485/251‑1 (MQ=255)                                                                                                                      gTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCaa                                                                                                                    >  1:339656/1‑251 (MQ=255)                                                                                                                                   ccGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTcgccgc                                                                                                        >  1:877915/1‑250 (MQ=255)                                                                                                                                   ccGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTcgccgc                                                                                                        >  1:218001/1‑250 (MQ=255)                                                                                                                                   ccGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCgccgcc                                                                                                       <  1:669309/251‑1 (MQ=255)                                                                                                                                           ccGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACg                                                                                                >  1:11319/1‑250 (MQ=255)                                                                                                                                             gTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGttgttg                                                                                                                                  <  1:503555/214‑1 (MQ=255)                                                                                                                                             gTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGc                                                                                                                                 >  1:564657/1‑215 (MQ=255)                                                                                                                                             gTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGc                                                                                               <  1:554057/249‑1 (MQ=255)                                                                                                                                             gTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCtt                                                                                             >  1:47234/1‑251 (MQ=255)                                                                                                                                             gTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCtt                                                                                             >  1:211558/1‑251 (MQ=255)                                                                                                                                                    aacCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAgctg                                                                                      <  1:901725/251‑1 (MQ=255)                                                                                                                                                       cACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATc                                                                                                                                                       <  1:842130/183‑1 (MQ=255)                                                                                                                                                           ggACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGcttctt                                                                                >  1:142398/1‑250 (MQ=255)                                                                                                                                                           ggACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGcttctt                                                                                >  1:263508/1‑250 (MQ=255)                                                                                                                                                             aCAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGCCCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTcg                                                                              >  1:432300/1‑250 (MQ=255)                                                                                                                                                                aaaCCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTcgcgcg                                                                          <  1:342444/251‑1 (MQ=255)                                                                                                                                                                 aaCCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGt                                                                         >  1:832988/1‑251 (MQ=255)                                                                                                                                                                       gAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAAt                                                                   >  1:205437/1‑251 (MQ=255)                                                                                                                                                                           aaaTTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGc                                                               <  1:204642/251‑1 (MQ=255)                                                                                                                                                                           aaaTTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGc                                                               <  1:799472/251‑1 (MQ=255)                                                                                                                                                                            aaTTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGcc                                                              <  1:725226/251‑1 (MQ=255)                                                                                                                                                                            aaTTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGcc                                                              <  1:570973/251‑1 (MQ=255)                                                                                                                                                                             aTTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGccc                                                             <  1:145354/251‑1 (MQ=255)                                                                                                                                                                             aTTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGccc                                                             <  1:637199/251‑1 (MQ=255)                                                                                                                                                                                tCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTg                                                           >  1:137855/1‑250 (MQ=255)                                                                                                                                                                                  aaCTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTgcgc                                                        <  1:306638/251‑1 (MQ=255)                                                                                                                                                                                  aaCTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTgcgc                                                        <  1:842249/251‑1 (MQ=255)                                                                                                                                                                                   actgactgAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGcc                                                       <  1:422658/251‑1 (MQ=255)                                                                                                                                                                                       actgaACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCaa                                                     <  1:831466/249‑1 (MQ=255)                                                                                                                                                                                         tgaACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGcc                                                 <  1:152509/251‑1 (MQ=255)                                                                                                                                                                                            acacTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTg                                               >  1:74228/1‑250 (MQ=255)                                                                                                                                                                                            acacTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTg                                               >  1:421457/1‑250 (MQ=255)                                                                                                                                                                                            acacTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTg                                               >  1:23373/1‑250 (MQ=255)                                                                                                                                                                                             cacTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGc                                                               >  1:125981/1‑233 (MQ=255)                                                                                                                                                                                              acTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGcgc                                            <  1:829870/251‑3 (MQ=255)                                                                                                                                                                                              acTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCt                                             >  1:472348/1‑250 (MQ=255)                                                                                                                                                                                                             ttGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGcggtcgg                             >  1:516550/1‑251 (MQ=255)                                                                                                                                                                                                               gTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAgct                                                                                       <  1:759621/191‑1 (MQ=255)                                                                                                                                                                                                               gTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAgc                                                                                        >  1:280671/1‑190 (MQ=255)                                                                                                                                                                                                               gTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGcc                                                 <  1:506570/229‑1 (MQ=255)                                                                                                                                                                                                               gTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGtt                           >  1:208341/1‑251 (MQ=255)                                                                                                                                                                                                               gTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGtt                           >  1:343204/1‑251 (MQ=255)                                                                                                                                                                                                               gTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGtt                           >  1:312033/1‑251 (MQ=255)                                                                                                                                                                                                                    aGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAg                                                                                                                                          <  1:845226/135‑1 (MQ=255)                                                                                                                                                                                                                    aGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGc                                                               >  1:62533/1‑210 (MQ=255)                                                                                                                                                                                                                    aGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCaa                      >  1:665833/1‑251 (MQ=255)                                                                                                                                                                                                                      gTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCaa                      <  1:74044/249‑1 (MQ=255)                                                                                                                                                                                                                      gTTAAACGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGAGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAAt                                                                   >  1:392904/1‑204 (MQ=255)                                                                                                                                                                                                                           aCGATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAacac               <  1:308497/251‑1 (MQ=255)                                                                                                                                                                                                                             gATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGcc                                                       >  1:710148/1‑209 (MQ=255)                                                                                                                                                                                                                             gATCCGCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGcc                                                       >  1:196121/1‑209 (MQ=255)                                                                                                                                                                                                                                  gCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAggg                                                                                                                                        <  1:668951/123‑1 (MQ=255)                                                                                                                                                                                                                                  gCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGtt        >  1:502516/1‑251 (MQ=255)                                                                                                                                                                                                                                  gCCTTATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGtt        >  1:134437/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttATTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGtttt      >  1:802010/1‑250 (MQ=255)                                                                                                                                                                                                                                       aTTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGc                                                  <  1:552564/204‑1 (MQ=255)                                                                                                                                                                                                                                       aTTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCa   >  1:841284/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTTCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCa   >  1:775757/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttCACAAGCGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCAc  <  1:183771/251‑1 (MQ=255)                                                                                                                                                                                                                                                cGGCTTATCGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTg                                    <  1:318048/209‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           TTAAAACGCGCCTGAAAACGCCGCACCGTTTGTGGCGTTAAACTGATTTCCGGCACCAACACCAGCACTTGCTTGCCTTGCTGCAAAATCTCTTCGATAAATTGCAAATAGATTTCCGTTTTGCCGGAACCCGTCACGCCGTCCAACAACCACGGGACAAAACCCTGAGTAAATTTCAACTGACTGAACACTAACGCCTGCGACTTGTTTAAGGTTAAACGATCCGCCTTATTCACAAGCGGCTTATTGCCTAATTGTTGCTGCCAGGTTTGGATTTCCGTTTCCAGAGCAACTTCCGTGACGTAATCTTTGGCTTTCAGCGCCGACCAAATCGCCGCACTAAAAGGGTTGTTGCCTTTCTCCGGCAAATCATTCGCCGCCTGCAACGCTTCAAGCTGCTTCTTCGCGCGTTTCAATAAGCCCTGCGCCAATGCCTGCTCACCAAGTGCGGTCGGTTTCCAAAAAACACTTGGGTTTTCCAC  >  1/582566‑583047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑