Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 565,297 G→A intergenic (+88/‑206) unknown → / → unknown Z‑ring‑associated protein ZapA/5‑formyltetrahydrofolate cyclo‑ligase (EC 6.3.3.2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1565,2970GA100.0% 201.1 / NA 71intergenic (+88/‑206)unknown/unknownZ‑ring‑associated protein ZapA/5‑formyltetrahydrofolate cyclo‑ligase (EC 6.3.3.2)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/33);  total (38/33)

AGTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAGTAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAACCGCCAC  >  1/565050‑565528
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       
aGTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATaa                                                                                                                                                                                                                                      >  1:108038/1‑251 (MQ=255)
 gTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATtaaataaa                                                                                                                                                                                                                                     >  1:744643/1‑251 (MQ=255)
   ggATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAg                                                                                                                                                                                                                                   >  1:723545/1‑251 (MQ=255)
   ggATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAg                                                                                                                                                                                                                                   >  1:797549/1‑251 (MQ=255)
    gATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAg                                                                                                                                                                                                                                   <  1:898013/250‑1 (MQ=255)
     aTAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGaa                                                                                                                                                                                                                                 <  1:570282/251‑1 (MQ=255)
               ctcCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       <  1:755493/251‑1 (MQ=255)
               ctcCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:766570/1‑251 (MQ=255)
               ctcCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:78862/1‑251 (MQ=255)
                         gCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAg                                                                                                                                                                                                             >  1:66732/1‑251 (MQ=255)
                          cccTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAg                                                                                                                                                                                                             <  1:214086/250‑1 (MQ=255)
                                            aaCTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGc                                                                                                                                                                                           <  1:581317/250‑1 (MQ=255)
                                              cTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGa                                                                                                                                                                                        >  1:499886/1‑251 (MQ=255)
                                              cTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGa                                                                                                                                                                                        >  1:412087/1‑251 (MQ=255)
                                                  tGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTcc                                                                                                                                                                                                                            >  1:19098/1‑211 (MQ=255)
                                                            gAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTa                                                                                                                                                                          >  1:541725/1‑251 (MQ=255)
                                                             aaaaaaaCGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATc                                                                                                                                                                             <  1:614301/247‑1 (MQ=255)
                                                                          tAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGc                                                                                                                                                                                           >  1:880790/1‑220 (MQ=255)
                                                                           aaTTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTg                                                                                                                                                                                                                          >  1:81977/1‑188 (MQ=255)
                                                                            aTTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTGAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTc                                                                                                                                                              >  1:454152/1‑247 (MQ=255)
                                                                                 ggATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATAc                                                                                                                                                                                     <  1:48308/219‑1 (MQ=255)
                                                                                   aTGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGgt                                                                                                                                                   <  1:884883/251‑1 (MQ=255)
                                                                                               ataaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGTTGTTCGCCAGTGGGTTACTTCCTTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTgctgtctcttatccaaatct                                                                                                                                        >  1:305206/1‑231 (MQ=255)
                                                                                               ataaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAg                                                                                                                                       >  1:614524/1‑251 (MQ=255)
                                                                                               ataaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAg                                                                                                                                       >  1:845317/1‑251 (MQ=255)
                                                                                                 aaaatCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCt                                                                                                                                     <  1:763882/251‑1 (MQ=255)
                                                                                                         gCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTCCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGgtg                                                                                                                                                  <  1:452089/230‑1 (MQ=255)
                                                                                                          cGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTaaga                                                                                                                            <  1:171472/251‑1 (MQ=255)
                                                                                                                  ggATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:349068/1‑152 (MQ=255)
                                                                                                                          ctctcGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCt                                                                                                                                          <  1:878161/221‑1 (MQ=255)
                                                                                                                            ctcGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAAttt                                                                                                                                                                                                                              <  1:169728/135‑1 (MQ=255)
                                                                                                                               gAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGc                                                                                                                                           >  1:477841/1‑215 (MQ=255)
                                                                                                                                aaaaTATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACt                                                                                                       <  1:64801/250‑1 (MQ=255)
                                                                                                                                aaaaTATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTa                                                                                                      >  1:440496/1‑251 (MQ=255)
                                                                                                                                      tCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGt                                                                                                                                                          <  1:658127/193‑1 (MQ=255)
                                                                                                                                           gcgcCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGaa                                                                                           >  1:582911/1‑251 (MQ=255)
                                                                                                                                            cgcCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAAc                                                                                          <  1:57372/251‑1 (MQ=255)
                                                                                                                                            cgcCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCAGTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAAc                                                                                          <  1:424681/251‑1 (MQ=255)
                                                                                                                                                gAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTa                                                                                      >  1:809739/1‑251 (MQ=255)
                                                                                                                                                   gTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTc                                                                                                                                                                                                  >  1:487178/1‑140 (MQ=255)
                                                                                                                                                    ttCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAggg                                                                                   <  1:287340/250‑1 (MQ=255)
                                                                                                                                                      cAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGtt                                                                                 >  1:776930/1‑250 (MQ=255)
                                                                                                                                                           ccTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       >  1:83039/1‑111 (MQ=255)
                                                                                                                                                            cTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgac                                                                          <  1:236441/251‑1 (MQ=255)
                                                                                                                                                              gagaATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgaccg                                                                        >  1:255746/1‑251 (MQ=255)
                                                                                                                                                                  aTTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGgtg                                                                                                                                                                                                                     <  1:376663/106‑1 (MQ=255)
                                                                                                                                                                            gATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACgg                                                          >  1:584311/1‑251 (MQ=255)
                                                                                                                                                                             aTTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGtt                                                                                                                                                                <  1:760552/148‑1 (MQ=255)
                                                                                                                                                                                       aCTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCg                                                   <  1:721832/247‑1 (MQ=255)
                                                                                                                                                                                       aCTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGT‑CCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCt                                                                                                                                     >  1:11675/1‑164 (MQ=255)
                                                                                                                                                                                       aCTAAAAGTATTCTGAGAAAAAGAATTTGAGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGt                                               >  1:851017/1‑251 (MQ=255)
                                                                                                                                                                                           aaaGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGCAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGtctc                                            >  1:438076/1‑250 (MQ=255)
                                                                                                                                                                                              gTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgac                                                                          >  1:123885/1‑217 (MQ=255)
                                                                                                                                                                                               tATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTAc                                        <  1:774983/250‑1 (MQ=255)
                                                                                                                                                                                                aTTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAg                                                                                     >  1:805181/1‑204 (MQ=255)
                                                                                                                                                                                                aTTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCa                                      >  1:630776/1‑251 (MQ=255)
                                                                                                                                                                                                   cTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAaat                                   <  1:227094/251‑1 (MQ=255)
                                                                                                                                                                                                     gagaAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCt                                                                                                                                     <  1:222091/151‑1 (MQ=255)
                                                                                                                                                                                                     gagaAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGga                                                                           <  1:382428/209‑1 (MQ=255)
                                                                                                                                                                                                             gAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAgg                                                                                                                                                                                                                       <  1:454605/61‑1 (MQ=255)
                                                                                                                                                                                                                   gtgCTAGTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGca                   >  1:317893/1‑251 (MQ=255)
                                                                                                                                                                                                                         gTCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTaaga                                                                                                                            <  1:734565/140‑1 (MQ=255)
                                                                                                                                                                                                                          tCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCaa                      >  1:806168/1‑241 (MQ=255)
                                                                                                                                                                                                                          tCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATcc              <  1:407987/249‑1 (MQ=255)
                                                                                                                                                                                                                          tCATTAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCtt            >  1:37247/1‑251 (MQ=255)
                                                                                                                                                                                                                              tAAACGTAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAAc        <  1:536863/251‑1 (MQ=255)
                                                                                                                                                                                                                                    tAATTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAACCGCcac  <  1:587039/251‑1 (MQ=255)
                                                                                                                                                                                                                                      aTTTCGAGTAGTATTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGgac                                                                          <  1:728872/177‑1 (MQ=255)
                                                                                                                                                                                                                                                gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATc                                                                                                                                                                             >  1:57258/1‑68 (MQ=255)
                                                                                                                                                                                                                                                gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTcc                                                                                                                                                             >  1:685416/1‑84 (MQ=255)
                                                                                                                                                                                                                                                gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTc                                                                                                                                    <  1:423963/109‑1 (MQ=255)
                                                                                                                                                                                                                                                gtaTTAAATAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCa          >  1:370203/1‑231 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       
AGTGGATAAAGTGCTCTCCATCGTTGCCCTGAATTTAAGTTTTGAACTCATGCAGGAACAGAAAAAAACGCATTTAATTGAGGATGTATTGAAAAATAAAATCCTGCGTCCGCTGGATCATTCTCTCGAAAATATCGGTGCGCCGAAGTTCAATTCCTGAGAATTTCACCGTGATTTCAGATAACTAAAAGTATTCTGAGAAAAAGAATTTGTGCTAGTCATTAAACGTAATTTCGAGTAGTATTAAGTAAAAGAATTTCCTGAGGTGTTCGCCAGTGGGTTACGTCCCTGAGCCGATACTTTTAATCTTATGAATTGGTTTCCTGTCATTGGTGTGCAGGCTTAGCTCGACTAAGAAGCCTAAAAATGATTTCAACTAATTCCCTTGAACCGTAGGGTTCAAGGACCGACCACCCGCAACGGCACCTCGGGGTCTCCTACCAAATAATACCTATGCAAGCACATCCTTCAACCGCCAC  >  1/565050‑565528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑